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Novel strain of Bacillus subtilis, and use thereof for preventing root rot disease in plants

A technology of Bacillus subtilis and root rot pathogen, which is applied in the field of new Bacillus subtilis strains and its application to the prevention and treatment of plant root rot, can solve the problems of low pesticide control value, polluting raw material ginseng, polluting water quality and soil, etc. Achieve the effect of reducing operating costs, improving national competitiveness, and reducing environmental pollution

Inactive Publication Date: 2014-04-02
KOREA RES INST OF BIOSCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to prevent root rot, many researchers at home and abroad have tried a variety of breeding methods and chemically synthesized pesticides for prevention and control, but the control value of the screened pesticides is low, so it is difficult to use, and some farmers abuse pesticides indiscriminately , pollute the raw material ginseng, and even pollute the water quality and soil

Method used

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  • Novel strain of Bacillus subtilis, and use thereof for preventing root rot disease in plants
  • Novel strain of Bacillus subtilis, and use thereof for preventing root rot disease in plants
  • Novel strain of Bacillus subtilis, and use thereof for preventing root rot disease in plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Screening for antagonistic microorganisms

[0056] In order to search for antagonistic microorganisms that inhibit the growth of pathogenic bacteria of ginseng root rot, the present inventors screened microorganisms from soil samples in Eumseong, South Chungcheong Province. The microorganisms can grow in substances containing the inhibitory activity produced by the pathogenic bacteria of root rot, and then the antagonistic microorganisms are screened out from the above microorganisms. The search for antagonistic microorganisms that inhibit the growth of pathogenic bacteria of ginseng root rot is carried out according to the following method. The soil samples were diluted with distilled water in different gradients, and then the substance with hindering activity produced by the pathogenic bacteria of root rot was smeared on the isolation medium, so as to screen and isolate candidate strains.

[0057] The microbial isolation medium used by the inventors (yeast...

Embodiment 2

[0059] Example 2: Identification of the antagonistic microorganisms explored

[0060] figure 2 The sequence (SEQ ID NO.1) of the gene (rDNA) encoding the 16SrRNA of the antagonistic microorganism strain screened in the above-mentioned Example 1 is shown. Compared with the known rDNA sequences of Bacillus subtilis AB017589 and AB017590, the rDNA sequence of Bacillus subtilis screened in the above Example 1 described as SEQ No. 1 has 99% similarity to the known Bacillus subtilis strain AB017589 , while 99% identical to another known Bacillus subtilis strain, AB107590.

[0061] The morphology of the Bacillus subtilis strains screened in the above-mentioned Example 1 was confirmed with an optical microscope, and then reconfirmed with an electron microscope at a magnification of 8800 times, and the average size of the strains was 0.6-0.7×1.5-1.6 About μm, slightly smaller than the general Bacillus subtilis with a size of 1.2-2.5μm, it has a flagella, and its length is about 7.5μ...

Embodiment 3

[0063] Embodiment 3: the culture condition of Bacillus subtilis B-5604

[0064] Antagonist microorganisms with activity of inhibiting ginseng root rot, that is, Bacillus subtilis B-5604, can be grown in LB medium (1% casein peptone, 0.5% yeast extract, 0.5% salt, after autoclaving) at 28°C , after 24 hours of shaking culture, the obtained culture solution was mixed with 20% glycerol at a ratio of 1:1, and 2ml was added to a cryogenic bottle, which was stored frozen at -70°C and used for the production and cultivation of active substances.

[0065] In order to produce an active substance that has inhibitory activity against ginseng root rot from active microorganisms, a cryogenic flask of frozen Bacillus subtilis B-5604 was dissolved and inoculated into 100 ml of active substance production medium (soluble starch 1%, Palmamed 0.5%, glucose 0.2%, ammonium sulfate 0.1%, potassium phosphate 0.1%, magnesium sulfate heptahydrate 0.05%, calcium chloride 0.1%, sodium chloride 0.3%...

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Abstract

The invention relates to a novel strain of Bacillus subtilis, and more particularly, to the B-5604 strain of Bacillus subtilis, which inhibits the growth of root rot pathogens, to a composition containing the strain or the culture medium thereof for controlling root rot pathogens, to a method for controlling ginseng root rot pathogens using the composition, and to a method for inhibiting the growth of Cylindrocarpon destructans or Fusarium solani using the strain. The microorganisms having antagonistic activity developed according to the present invention are not harmful to water and soil, and thus does not cause environmental pollution, and particularly, the microorganisms having antagonistic activity according to the invention may not only reduce root rot pathogens in farmland in which ginseng is repeatedly cultivated or recultivated, but also alleviate root rot disease in ginseng being cultivated, thus significantly contributing to ginseng cultivation.

Description

technical field [0001] The present invention relates to a novel bacillus subtilis bacterial strain, more specifically to a kind of bacillus subtilis bacterial strain B-5604 which hinders the growth of root rot pathogenic bacteria, and a composition for preventing and treating root rot pathogenic bacteria comprising the above bacterial strain or its culture solution , and a method for controlling the pathogenic bacteria of ginseng root rot by using the above-mentioned composition, and a method for inhibiting the growth of rust-rot fungus or Fusarium solani by using the above-mentioned strain. Background technique [0002] Before 1992, the research on root rot of plants was mainly carried out on a generalized root rot caused by Cylindrocarpon destructans (Zinssm.) Scholten, in which rust rot fungi are compound Root rot and ginseng root rot pathogens, and the research on root rot in the real sense has been concentrated since 1992. However, there are still very few research res...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02C12R1/125A01N63/22
CPCA01N63/00C12R1/125A01N63/22C12R2001/125C12N1/205C12N1/20
Inventor 金永国金重秀金亨津裴京淑
Owner KOREA RES INST OF BIOSCI & BIOTECH
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