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Foot-and-mouth disease virus detecting test paper tape and its preparation method and using method

A foot-and-mouth disease virus and test strip technology, which is applied in the field of foot-and-mouth disease virus stereotyped diagnostic test strips, can solve the problems of being unfavorable to the development of epidemic prevention and control, high cost, complicated operation, etc., and achieves the effects of intuitive and easy determination and simple operation.

Active Publication Date: 2006-12-13
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods not only require certain equipment and laboratory facilities, but also require technical personnel with relevant experimental skills to operate.
At the same time, the operation is complicated, the process is numerous, and the cost is high, which is not conducive to the grassroots and field FMD epidemiological investigation and epidemic prevention and control.

Method used

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  • Foot-and-mouth disease virus detecting test paper tape and its preparation method and using method
  • Foot-and-mouth disease virus detecting test paper tape and its preparation method and using method
  • Foot-and-mouth disease virus detecting test paper tape and its preparation method and using method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Preparation of O-type foot-and-mouth disease diagnostic test strips

[0070] 1. Preparation of colloidal gold

[0071] Using trisodium citrate reduction method, take 100mL of 0.01% chloroauric acid aqueous solution and heat to boiling, accurately add 2mL of 1% trisodium citrate aqueous solution under stirring, the golden yellow chloroauric acid aqueous solution turns purple within 2min, continue to boil After cooling for 15 minutes, restore the original volume with distilled water. The gold sol prepared in this way has the highest absorption peak in the visible light region at 523nm, A1cm / 523=1.188. Add 0.02% NaN to the prepared colloidal gold 3 , filtered through a disposable sterilizing filter, and put into a clean glass bottle for later use. The diameter of the colloidal gold prepared in the work is observed by the particle size in the transmission electron microscope (TEM image). The measured average particle size is 30.06±0.7nm, the results are as follows figu...

Embodiment 2

[0082] Preparation of test strips for diagnosis of type A foot-and-mouth disease

[0083] 1. Preparation of colloidal gold

[0084] Using trisodium citrate reduction method, take 100mL of 0.01% chloroauric acid aqueous solution and heat to boiling, accurately add 2mL of 1% trisodium citrate aqueous solution under stirring, the golden yellow chloroauric acid aqueous solution turns purple within 2min, continue to boil After cooling for 15 minutes, restore the original volume with distilled water. The gold sol prepared in this way has the highest absorption peak in the visible light region at 523nm, A1cm / 523=1.188. Add 0.02% NaN to the prepared colloidal gold 3 , filtered through a disposable sterilizing filter, and put into a clean glass bottle for later use. The diameter of the colloidal gold prepared in the work is observed by the particle size in the transmission electron microscope (TEM image). The measured average particle size is 30.06±0.7nm, the results are as follows ...

Embodiment 3

[0095] Preparation of C-type foot-and-mouth disease diagnostic test strips

[0096] 1. Preparation of colloidal gold

[0097] Using trisodium citrate reduction method, take 100mL of 0.01% chloroauric acid aqueous solution and heat to boiling, accurately add 2mL of 1% trisodium citrate aqueous solution under stirring, the golden yellow chloroauric acid aqueous solution turns purple within 2min, continue to boil After cooling for 15 minutes, restore the original volume with distilled water. The gold sol prepared in this way has the highest absorption peak in the visible light region at 523nm, A1cm / 523=1.188. Add 0.02% NaN to the prepared colloidal gold 3 , filtered through a disposable sterilizing filter, and put into a clean glass bottle for later use. The diameter of the colloidal gold prepared in the work is observed by the particle size in the transmission electron microscope (TEM image). The measured average particle size is 30.06±0.7nm, the results are as follows figu...

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Abstract

Disclosed are a method for preparing and virus type definition test paper for foot and mouth disease and the using method. The test paper comprises O, A, Asia l, C four kinds of serum detecting test paper. The test paper comprises a PVC substrate plate, nitrocellulose membrane, an absorbing pad, a golden mark pad and a sample pad. The PVC substrate plate is installed at the most bottom layer; the nitrocellulose membrane is set on the middle part of the substrate plate; the absorbing pad is stuck on the left end of the nitrocellulose membrane, and the golden mark pad is set on the right end of the nitrocellulose membrane; the sample pad is set on the right upper end of the golden pad. The invention can identify the foot and mouth virus O, A, Asia l and C four serum type.

Description

technical field [0001] The invention relates to the field of rapid and accurate diagnosis of the serotype of the foot-and-mouth disease virus, in particular to a test strip for the stereotyping diagnosis of the foot-and-mouth disease virus, and the invention includes methods for preparing and using the test strip. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, contagious infectious disease caused by foot-and-mouth disease virus (FMDV). It is so large that it ranks among the most among many diseases. The International Office of Epizootics (OIE) listed it as the first class A severe infectious disease. Because the outbreak of foot-and-mouth disease has affected international relations, national reputation and the economic development of countries all over the world, some people call this disease "political and economic disease". Foot-and-mouth disease virus has 7 serotypes of O, A, C, Asia I, SAT1, SAT2 and SAT3, and more than 80 subtypes. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/52G01N33/544G01N33/577G01N33/532
Inventor 蒋韬梁仲刘湘涛刘在新陈涓马军武
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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