The invention discloses a virus purifying and amplification method. The method comprises steps: firstly, cell fragments in viruses are removed; secondly, the viruses are captured through a chromatography; thirdly, the viruses captured in the second step are processed by nucleases, host cell nucleic acids in the viruses are subjected to enzymolysis; fourthly, the viruses processed in the third steps are subjected to fined purifying, host cell protein and nucleic acid fragments are removed. The chromatography is employed to achieve virus purifying and amplification, and optimization is carried out at the chromatography column and chromatography filling material aspects. When the provided method is compared with traditional virus purifying method, the purity and the titer of the viruses after purifying are raised, and the provided method has advantages of easy amplification and low cost. The obtained viruses with low residues, high purity and high titer are used for virus removal verification, a flux of a virus filtering membrane can be raised, and the cost investment of the virus filtering membrane is lowered.