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Panax root extract detection method

A kind of Acanthopanax extract and detection method technology, which is applied in the direction of drug combination, medical formula, plant raw materials, etc., can solve problems affecting product production and quality assurance, simple quality control methods, and difficult product quality control, etc., to achieve accuracy High, high sensitivity, high precision effect

Inactive Publication Date: 2011-05-11
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing Acanthopanax extracts have the disadvantages of simple quality control methods and difficult control of product quality, which will affect the production and quality assurance of products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Determination of the content of syringin in the extract of Acanthopanax senticosus by HPLC

[0036] Determination according to high performance liquid chromatography (Appendix VID)

[0037] The chromatographic strip and system suitability test use octadecyl bonded silica gel as the filler, methanol-water (20:80) as the mobile phase, the detection wavelength is 265nm, and the theoretical plate number should be calculated according to the syringin peak. less than 2000.

[0038] Preparation of Reference Substance Solution Accurately weigh an appropriate amount of syringin standard substance, add methanol to dilute to a solution containing 80ug per 1ml, and obtain it.

[0039] Preparation of the test solution: Take about 0.20 g of the Acanthopanax spinosa extract powder, accurately weigh it, put it in a 50ml measuring bottle, add an appropriate amount of methanol to dissolve and dilute to the mark, shake well, filter, and take the filtrate to obtain the final product.

[...

Embodiment 2

[0044] The extract of Acanthopanax senticosus and the standard product of Acanthopanax senticosus were measured by mid-infrared spectrum, and the similarity of one-dimensional spectra was compared. The specific steps are as follows:

[0045] 2. Sample processing: Take 100g of Acanthopanax senticosus extract, crush it, and pass it through a 80-mesh sieve for later use.

[0046] 3. Instrument Preparation

[0047] 3.1 Turn on the instrument: Adjust the power switch button on the right rear side of the main body of the mid-infrared spectrometer to the "ON" state, wait for about 15 seconds, and wait for the four indicators on the left side of the instrument panel to turn green.

[0048] 3.2 Program start: Double-click the icon marked "Spectrum v5.3.1" on the desktop to enter the "Login" state; when online, the "Instrument" option is in the "1.Spectrum One" state; when not online, the "Instrument" option is in the "Work Offline" state. (Other items such as initialization display d...

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Abstract

The invention relates to a medicament detection method, in particular to a panax root extract detection method. The detection method comprises: measuring the content of a syringin component; and comparing the intermediate infrared one-dimensional spectrum of the panax root extract and the intermediate infrared one-dimensional spectrum of a reference product.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a detection method of Acanthopanax japonicus extract. Background technique [0002] Acanthopanax extract is a light brown fine powder prepared by extracting and processing the dried roots and rhizomes of Acanthopanax. Active ingredients: eleutheroside B and eleutheroside E. Main functions: nourishing qi and strengthening the spleen, nourishing the kidney and calming the nerves. For spleen and kidney yang deficiency, physical weakness and fatigue, loss of appetite, soreness of the waist and knees, insomnia and dreaminess. [0003] Acanthopanax extract is a prior art, and its preparation, detection and application can be found in Chinese Pharmacopoeia and other documents. [0004] The existing Acanthopanax extract has the disadvantages that the quality control method is simple and the product quality is not easy to control, which will affect the production and quality assurance of the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/254A61P1/14A61P13/12A61P25/20A61K125/00
Inventor 刘岩徐波王俊全牛涛陈庆闯
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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