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Method for constructing Bidens parviflor willd medicinal material capillary electrophoresis fingerprint and application thereof

A technology of capillary electrophoresis and construction method, which is applied in the field of construction of fingerprints of herbal medicinal materials, can solve the problems of consuming toxic organic solvents, damaging chromatographic columns, polluting the environment, etc., and achieves the effect of perfecting the quality evaluation system

Inactive Publication Date: 2010-11-24
深圳市药品检验所
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Problems solved by technology

The chromatographic fingerprint quality control technology of traditional Chinese medicine is an effective means for overall and macroscopic analysis of traditional Chinese medicine. Among them, HPLC fingerprint is the most widely used, but the extract of traditional Chinese medicine often damages the chromatographic column and consumes a large amount of toxic organic solvents. Analysis is expensive and pollutes the environment

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  • Method for constructing Bidens parviflor willd medicinal material capillary electrophoresis fingerprint and application thereof
  • Method for constructing Bidens parviflor willd medicinal material capillary electrophoresis fingerprint and application thereof
  • Method for constructing Bidens parviflor willd medicinal material capillary electrophoresis fingerprint and application thereof

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Embodiment Construction

[0019] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention.

[0020] 1. Instruments and reagents

[0021] HP3DCE high-performance capillary electrophoresis instrument (Agilent Technology Co., Ltd., USA), G1600-60210 quartz capillary (Agilent Technology Co., Ltd., USA), and PTS-1002 ultrasonic instrument (Shenzhen Boda Ultrasonic Equipment Co., Ltd.).

[0022] Boric acid (analytical pure, Xilong Chemical Factory, Shantou City, Guangdong), borax (analytical pure, Xilong Chemical Factory, Shantou City, Guangdong), sodium hydroxide (analytical pure, Guangzhou Chemical Branch), sodium dihydrogen phosphate (analytical pure, Guangzhou Chemical branch), disodium hydrogen phosp...

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Abstract

The invention provides a method for constructing a Bidens parviflor willd medicinal material capillary electrophoresis fingerprint and an application thereof. The construction method comprises the steps of preparing a test sample solution, preparing a reference solution and analyzing capillary electrophoresis, wherein the reference product is one or more of salicylic acid, rutin or hyperin. The invention radicates the construction method of the Bidens parviflor willd medicinal material fingerprint, can obtain the Bidens parviflor willd medicinal material fingerprint by the method, improve the quality evaluation system of the Bidens parviflor willd medicinal material, overcome the defects of damaged chromatographic columns, large used amount of toxic organic solvents, high analysis cost and polluted environment in the HPLC fingerprint, and provide theory and practice basis for the Bidens parviflor willd medicinal material and overall and effective quality control of crude slices thereof.

Description

technical field [0001] The present invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for constructing fingerprints of the herbal medicinal herb, and its application. Background technique [0002] In the process of research on the modernization of traditional Chinese medicine, how to effectively evaluate the quality of traditional Chinese medicinal materials has always been an important and difficult issue in the research and application of traditional Chinese medicine. With the development of science and technology, fingerprint quality control technology has been widely used in the quality control of Chinese medicinal materials and their extract preparations. The chromatographic fingerprint quality control technology of traditional Chinese medicine is an effective means for overall and macroscopic analysis of traditional Chinese medicine. Among them, HPLC fingerprint is the most widely used, but the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28G01N30/88A61P11/04A61P1/12A61P31/00A61P13/08A61P35/00
Inventor 李玉兰刘敏庞发根王珏王军德
Owner 深圳市药品检验所
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