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Mature wheat embryo callus induction medium and induction method thereof

A callus induction and callus technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of research, unclear components, and high cost

Active Publication Date: 2017-12-22
CHINA WEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, hydrolyzed casein is often added to the culture medium when inducing callus from mature wheat embryos [for example, Guo Xiaoli et al., Research on the Culture of Callus from Mature Embryos of Different Wheat Varieties, Anhui Agricultural Sciences, 2008, 36:173-174; Ding L etal, Optimization of Agrobacterium mediated transformation conditions in matureembryos of elite wheat. Mol Biol Rep, 2009, 36:29–36], hydrolyzed casein is a mixture of polypeptides and amino acids, rich in nutrients but not clear in composition, and High cost, not conducive to large-scale callus induction and induction mechanism research

Method used

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  • Mature wheat embryo callus induction medium and induction method thereof
  • Mature wheat embryo callus induction medium and induction method thereof
  • Mature wheat embryo callus induction medium and induction method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1 The inventive method induces wheat mature embryo callus

[0036] 1. The induction method is as follows:

[0037] Select ripe, plump, relatively consistent color and size mature seeds, sterilize them with 70% ethanol for 3 minutes, wash them with sterilized double distilled water for 5-6 times, and then soak them in hydrogen peroxide with a weight-to-volume ratio of 30% for 10 minutes , washed in sterilized double distilled water for 5-6 times and then soaked in double distilled water for 45 minutes. Dissect the seeds with a scalpel to expose mature embryos, cut off part of the embryos and inoculate them in culture dishes with the scutellum facing up in the dark for one week (25°C, 60% relative humidity). The structure was cut, and the completely white (light yellow) callus was picked out, and cultured in a new medium for more than 3 weeks (the composition of the new medium was the same as that of the above-mentioned medium), and each experiment was repeate...

Embodiment 2

[0045] Embodiment 2 The inventive method induces mature wheat embryo callus

[0046] 1. The induction method is as follows:

[0047] Except the amino acid concentration in the callus induction medium, others are the same as in Example 1.

[0048] Wherein, the amino acid and final concentration in the culture medium are respectively:

[0049] Glycine 60.0mg·L -1 , aspartic acid 6.0mg·L -1 , Proline 2.0mg·L -1 , leucine is 0.1mg·L -1 .

[0050] 2. Induction results: the healing rate of mature wheat embryos reached 93.3%.

[0051] The beneficial effect of the present invention is specified below by test example:

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Abstract

The invention provides a mature wheat embryo callus induction medium. An MS medium is used as a basic medium, and is added with acetosyringone with the final concentration of 50-100 mu M / L, 1.0-2.0 mg / L of 2,4-D and an amino acid ingredient to form the mature wheat embryo callus induction medium, and the amino acid ingredient consists of glycine, aspartic acid, proline and leucine. The invention further provides application of the medium and a method for inducing a mature wheat embryo to form a callus. The medium has high inductivity to the mature wheat embryo callus, comprises simple components and is low in preparation cost. The mature wheat embryo callus induction medium lays a good theoretical basis on high-efficiency tissue regeneration of wheat and research on amino acid metabolism, and is good in application prospect.

Description

technical field [0001] The invention belongs to the field of crop tissue culture, and in particular relates to a wheat mature embryo callus induction medium and an induction method thereof. Background technique [0002] As one of the three most important food crops in the world, wheat plays an important role in the development of agriculture and the improvement of people's living standards. With the development of genetic engineering and genomics, the use of modern genetic engineering technology to qualitatively transform wheat for the selection of new wheat varieties has attracted people's attention. However, wheat transgenic research lags behind the other two important food crops - rice and corn, which is largely due to the imperfect construction of wheat transformation system. [0003] So far, scholars have used a variety of different explant materials to induce callus in wheat tissue culture research, including anthers, young spikes, immature embryos, and mature embryos...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 杨在君吴一磊彭正松余燕魏淑红陈真勇廖明莉欧阳钟鸣
Owner CHINA WEST NORMAL UNIVERSITY
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