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Method for analyzing influence of taurine on growth of tilapia on basis of NMR metabonomics technology

A technology of metabolomics and taurine, applied in the field of analysis, can solve the problem that the mechanism of fish metabolism is not very clear

Inactive Publication Date: 2019-04-12
XIAMEN UNIV
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Problems solved by technology

However, the effects of adding taurine in feed on the metabolism of fish at different growth stages and the mechanism of action are still not very clear.

Method used

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  • Method for analyzing influence of taurine on growth of tilapia on basis of NMR metabonomics technology
  • Method for analyzing influence of taurine on growth of tilapia on basis of NMR metabonomics technology
  • Method for analyzing influence of taurine on growth of tilapia on basis of NMR metabonomics technology

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Embodiment Construction

[0025] Below in conjunction with specific implementation examples, further illustrate the present invention:

[0026] 1. Configuration of experimental feed

[0027] The present invention uses tilapia feed to be configured by the base feed (not containing taurine) containing 37% crude protein and 6% crude lipid, wherein protein source is casein and gelatin (not containing taurine), Lipid source is fish oil, soybean oil and soybean lecithin, carbohydrate source is corn starch (raw starch), other feed ingredients are vitamin premix, mineral premix, vitamin C, calcium dihydrogen phosphate, bile chloride Approximate composition of alkali, shrimp powder and microcrystalline cellulose. Taurine (food grade, Beijing Huikangyuan Biotechnology Co., Ltd.) was added to the basal feed at the ratio of 0.0%, 0.4%, 0.8% and 1.2%, respectively, and four kinds of Luo with different levels of taurine were prepared. For the non-fish experimental feed, the groups were respectively recorded as CTR...

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Abstract

The invention discloses a method for analyzing the influence of taurine on the growth of tilapia on the basis of NMR metabonomics technology. The method comprises: collecting fish muscle samples and collecting the one-dimensional nuclear magnetic resonance hydrogen spectrum of each sample to obtain the original spectrogram of each sample; performing data preprocessing on each original spectrogramto obtain a two-dimensional data matrix; performing multivariate statistical analysis on the two-dimensional data matrix to obtain small molecular metabolites of each tilapia muscle tissue under the influence of different taurine addition amounts; and obtaining a metabolite change law through the identification and pattern recognition analysis of the metabolites, and screening biomarkers related to sugar metabolism, lipid metabolism and amino acid metabolism. According to the method for analyzing the influence of the taurine on the growth of the tilapia on the basis of the NMR metabonomics technology, the nuclear magnetic resonance metabonomics technology is used for analyzing the influence of the taurine on the growth of the tilapia, so that the metabolic difference of tilapia muscles under the influence of different taurine addition amounts can be rapidly, accurately, and comprehensively obtained, and the addition accuracy of taurine feed in tilapia breeding and the influence on thegrowth of the tilapia can be effectively evaluated.

Description

technical field [0001] The invention belongs to the field of analysis technology, and in particular relates to a method for analyzing the influence of taurine on the growth of tilapia based on NMR (Nuclear Magnetic Resonance, nuclear magnetic resonance) metabolomics technology. Background technique [0002] Global aquaculture fishing and aquaculture production have maintained growth for 30 years, especially the rapid development of aquaculture, resulting in a shortage of fishmeal, which provides feed protein sources, and rising prices. Therefore, plant protein has become an option to replace fishmeal to reduce the cost of aquaculture, but the nutritional imbalance and anti-nutritional factors in plant protein are not ideal. Appropriate addition of taurine in plant protein feed can improve the adverse effects of plant protein substitution on fish and promote the growth and development of fish. However, the effects of adding taurine in feed on the metabolism of fish in differ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08
Inventor 沈桂平殷爽黄颖冯江华
Owner XIAMEN UNIV
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