Swine lactobacillus crispatus and application thereof

A technology of Lactobacillus crispatus, strains, applied in the field of microbiology

Active Publication Date: 2018-09-18
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] So far, there has been no report on the use of Lactobacillus crispatus in pig f

Method used

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  • Swine lactobacillus crispatus and application thereof
  • Swine lactobacillus crispatus and application thereof
  • Swine lactobacillus crispatus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The isolation of embodiment 1 lactic acid bacteria strain

[0036] Collect the fresh feces of suckling piglets, weigh 25g, place it in a glass beaded triangular flask filled with 225ml of sterile normal saline, shake it well, then draw 1.0ml of bacterial liquid into a flask filled with 9.0ml of sterile normal saline In test tubes, this dilution is 10 -1 , repeat the above process, and make 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 of bacteria liquid. choose 10 -4 、10 -5 、10 -6 For three dilutions, draw 0.1ml of the bacterial solution and drop it on the above-mentioned medium plate, culture in an anaerobic tank at 37°C for 24-48 hours, and pick typical colonies for API50 and 16SrDNA identification. According to the lactic acid bacteria fermenting sugar and producing acid to make the medium turn yellow to determine the sugar utilization of the strain, the colonies that produce yellow circles and different shapes are purified, cultured, Gram stained, microscopic examinati...

Embodiment 2

[0037] The screening test of embodiment 2 lactic acid bacteria strains

[0038] After the strain was activated, it was inoculated in MRS liquid medium, and after 24 hours of cultivation, a certain amount of bacterial liquid was taken to measure the OD600nm value, and the growth performance of the strain was observed with no inoculation as the control. At the same time, the strain was inoculated in MRS liquid medium and cultured for 24 hours, 5ml of fermentation broth was taken and centrifuged at 6000rpm for 5 minutes, added to 5ml of liquid medium containing 0.2% bile salt pH=3, incubated for 2h, and then inoculated with 10% Incubate in medium for 4h, and measure OD with culture without any treatment as control 600nm value, and calculate the acid-resistant and bile-resistant performance of the strain.

[0039] Screened by growth, acid and bile salt resistance tests, see figure 1 and figure 2 , confirm that the strain R2 (in the figure) is the target strain (the laboratory ...

Embodiment 3

[0040] The identification of embodiment 3 Lactobacillus crispatus

[0041] The strain R2 (the laboratory number is L.crispatus ZLC020) was sent to the China Industrial Microorganism Culture Collection Management Center for identification, and the physiological and biochemical characteristics (Table 1) and molecular biological identification confirmed that the strain was Lactobacillus crispatus ( Lactobacillus crispatus). The strain was tested on October 22, 2015 in the General Microbiology Center of China Microbiological Culture Collection Management Committee (Address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip Code: 100101) Preserved, the strain name is Lactobacillus crispatus (Lactobacillus crispatus) ZLC020, and its preservation number is CGMCC No.11531.

[0042] Table 1 Physiological and biochemical characteristics of pig-derived Lactobacillus crispatus

[0043]

[0044] Note: "+" in the ta...

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Abstract

The invention provides acid-resistant and bile-salt-resistant lactobacillus crispatus. The strain has the preservation name of lactobacillus crispatus ZLC020. The preservation number is CGMCC No.11531. The strain disclosed by the invention has excellent anti-bacterium performance; the obvious bacteriostatic effect is achieved on swine escherichia coli. The strain, the relevant strain and relevantfeed can improve the production performance of growth pig; the intestinal flora composition is improved; the body amino acid metabolism is promoted; good development and application prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a porcine-derived Lactobacillus crispatus and an application thereof. Background technique [0002] The long-term use of antibiotic growth promoters in large quantities in feed can lead to the enhancement of drug resistance of intestinal pathogenic bacteria, causing problems such as animal product safety and environmental pollution, and posing a threat to animal health and human health. The European Union has banned the addition of antibiotic growth promoters to animal feed since January 1, 2006. Banning antibiotics in food animal feed has become a worldwide consensus. Announcement No. 2428 of the Ministry of Agriculture of my country banned the use of colistin sulfate as a growth promoter on November 1, 2016. Announcement No. 2638 decided to stop the use of olaquindox, aminophenylarsinic acid, and roxarsone in May 2018. in food animal production. Therefore, the de...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23K10/18A23K50/30C12R1/225
CPCA23K10/18A23K50/30C12N1/04C12N1/20C12R2001/225C12N1/205A23V2400/133
Inventor 张董燕季海峰刘辉王四新王雅民
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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