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A kind of extract derived from animal tissue and its preparation method and application

A technology of animal tissues and extracts, which is applied in skin care preparations, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve the problems of harsh storage conditions, single use, and low extraction rate, and achieve wide application and extraction The effect of increasing the rate and stabilizing the extract

Active Publication Date: 2021-11-26
科索瑞生物科技(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the extracts prepared by the above methods have defects such as low stability, low extraction rate, inactivation of enzyme sources, carrying pyrogens, harsh storage conditions, and single use.

Method used

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  • A kind of extract derived from animal tissue and its preparation method and application
  • A kind of extract derived from animal tissue and its preparation method and application
  • A kind of extract derived from animal tissue and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of Extracts from Rat Embryo Tissue

[0034] The preparation method of the mouse embryo tissue source extract of the present invention comprises the following steps:

[0035] (1) Treatment of animal tissues: take healthy mice after inspection and quarantine, kill the pregnant mice by cervical dislocation, take out the mouse embryos by laparotomy, clean the mouse embryos with sterilized pure water, soak them in iodophor for 2 minutes, and take them out with a volume ratio Deiodine with 75% alcohol, wash 3 times with sterilized pure water, and mechanically homogenize under sterile conditions.

[0036](2) Enrichment of the extract: add 20 times the volume of sterilized pure water and 2 times the volume of 2.5g / L trypsin aqueous solution to the homogenate tissue obtained in step (1) to make a mixed solution, and the The mixture was incubated in an anaerobic incubator at 35° C. for 72 hours without adding any medium to perform starvation stimulation. ...

Embodiment 2

[0044] Example 2 Preparation of bovine umbilical cord tissue extract

[0045] The preparation method of bovine umbilical cord tissue source extract of the present invention comprises the following steps:

[0046] (1) Treatment of animal tissue: Take the isolated umbilical cord tissue of a healthy cow after natural delivery after inspection and quarantine, tie the two ends of the umbilical cord tightly with silk thread, soak it in normal saline, and transport it back to the laboratory at 4°C. After cleaning the umbilical cord tissue with sterilized pure water, remove both ends of the umbilical cord with sterile surgical scissors, soak in povidone iodine for 1 min, take it out, deiodine it with 75% alcohol by volume, rinse it with sterilized pure water for 3 times, and put it under sterile conditions. Under mechanical homogenization.

[0047] (2) Enrichment of the extract: add 30 times volume of sterilized pure water and 3 times volume of 2.5g / L trypsin aqueous solution to th...

Embodiment 3

[0055] Example 3 Preparation of Rabbit Placenta Tissue Extract

[0056] The preparation method of the rabbit placental tissue source extract of the present invention comprises the following steps:

[0057] (1) Treatment of animal tissue: Take healthy rabbits after inspection and quarantine, and after natural delivery in a clean room, collect the placenta tissue, clean it with sterilized pure water, and cut the placenta tissue with sterile scissors under aseptic conditions. Size 1cm 3 The blocks were transferred to povidone iodine and soaked for 2 minutes, then taken out and deiodized with 75% alcohol by volume, washed 3 times with sterilized pure water, and mechanically homogenized under sterile conditions.

[0058] (2) Enrichment of extract: add 40 times volume of sterilized pure water and 4 times volume of 2.5g / L trypsin aqueous solution to the homogenate tissue obtained in step (1) to make a mixed solution, and the The mixture was incubated in an anaerobic incubator at ...

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Abstract

The invention relates to an extract derived from animal tissue, a preparation method and application thereof. The method comprises the following steps: (1) homogenizing the animal tissue; (2) adding a low-concentration aqueous solution of trypsin to the homogenized tissue obtained in step (1) to prepare a mixed solution, without adding any medium Place it in an anaerobic incubator and incubate; (3) centrifuge the mixture after incubation in step (2), collect the supernatant while retaining the deposited tissue; (4) obtain the deposited tissue in step (3) Add a high-concentration trypsin aqueous solution to the substance, then stir, centrifuge, and collect the supernatant; (5) combine the supernatant collected in step (3) and step (4). The method of the invention can prepare the extract with high extraction rate, and the obtained extract has stable performance.

Description

technical field [0001] The invention belongs to the field of biotechnology. Specifically, the present invention relates to a method for preparing an extract from animal tissue, the extract prepared by the method and its use. Background technique [0002] Animal extract is a stable concentrate obtained from animal body, part of animal body tissue or viscera through extraction and processing. In the past, it was generally called biochemical products or biochemical raw materials. Its main component categories are: amino acids, peptides, proteins, enzymes and coenzymes, polysaccharides, lipids, nucleic acids and their derivatives, which can be mainly used in food, biopharmaceuticals, health care, medical treatment, beauty and so on. [0003] The raw materials of animal extracts are often supplied by slaughterhouses. The collection, storage and transportation of these animal tissues and organs are all important factors to ensure the quality, efficacy and safety of the extracts....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A61K8/98A61Q19/00
CPCA61K8/982A61K2800/805A61Q19/00C12P21/06
Inventor 高文勇邓鹏
Owner 科索瑞生物科技(天津)有限公司
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