Method for degrading organic pollutant through nanometer material photo-reduction and assisted by electricigens
A technology for generating electricity from microorganisms and organic pollutants, which is applied in the fields of sewage or sludge treatment, water, and wastewater. It can solve the problems of low degradation ability of refractory pollutants and achieve the effect of improving the repair ability
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[0027] The thallus S.oneidensis MR-1 of the present invention is a model of electrogenic microorganisms (Genome sequence of the dissimilatory metal ion–reducing bacterium Shewanella oneidensis.Nature Biotechnology.2002,20:1118-1123), developed by Nielsen University of California, USA Presented by the professor; the strain is preserved in the American Type Type Collection Center (ATCC), and the strain number is ATCC 700550 TM . This strain can be purchased directly from the center. The photoreduction nanomaterial used is silver phosphate nanoparticle; the degradation dye used is rhodamine B.
[0028] (1) Streak S.oneidensis MR-1 preserved in glycerol on LB plates and culture overnight at 30°C;
[0029] (2) Pick the activated single colony with a toothpick, inoculate it in 50ml of LB liquid medium, shake it at 30°C and 200rpm to the logarithmic phase, take the logarithmic phase bacterial liquid, centrifuge at 5000rpm for 15min, remove the supernatant, and use S. oneidensis MR...
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