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Anaerobic culture bag with anaerobic indicator

An anaerobic indicator and anaerobic culture technology, which are applied in specific-purpose bioreactors/fermenters, biomass post-processing, biochemical equipment and methods, etc., can solve the problems of medium waste and improve anaerobic The effect of testing efficiency

Active Publication Date: 2022-05-13
广东药科大学附属第一医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the air humidity is too high, water seeps in, the humidity of the culture gene is too high, and mold grows and becomes invalid; when the air humidity is too low, the water is lost, and the culture gene is too dry to fail; moreover, due to the infiltration of oxygen, the time is long, the oxygen cultivation The base forms peroxides that are harmful to anaerobic bacteria and make the medium useless

Method used

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  • Anaerobic culture bag with anaerobic indicator
  • Anaerobic culture bag with anaerobic indicator
  • Anaerobic culture bag with anaerobic indicator

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Embodiment Construction

[0024] Below in conjunction with accompanying drawing, the present invention will be further described.

[0025] figure 1 It is a structural schematic diagram of an embodiment of the anaerobic culture bag with an anaerobic indicator of the present invention. see figure 1 , the anaerobic culture bag includes a bag body 10 and an anaerobic indicator, and the anaerobic indicator is used to detect the anaerobic state of the gas in the bag body 10 .

[0026] The anaerobic indicator comprises a housing 20 , a first piston 30 and an opening device 40 , preferably arranged in the first piston 30 . The housing 20 has a piston chamber 21 , and the first piston 30 is located in the piston chamber 21 . The opening device 40 is arranged on the housing 20 and is used to connect the mouth 51 of the ampoule 50 containing the anaerobic indicator. When the pressure in the piston chamber 21 is higher than a preset value, the opening The device 40 is triggered to open the mouth 51 so that the...

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PUM

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Abstract

The invention relates to an anaerobic culture bag with an anaerobic indicator, which includes a bag body and an anaerobic indicator, the anaerobic indicator includes a casing, a first piston and an opening device, and the casing has a piston chamber The bag body is provided with a one-way valve, and when the gas pressure in the bag body is greater than the air pressure in the piston chamber, the gas in the bag body flows into the piston chamber through the one-way valve The opening device is arranged on the housing, and is used to connect the mouth of the ampoule bottle containing the anaerobic indicator. When the pressure in the piston chamber is higher than a preset value, the opening device is triggered to Open the mouth of the bottle so that the gas in the piston cavity enters the ampoule. The anaerobic culture bag with anaerobic indicator provided by the present invention allows the gas in the piston cavity to enter the ampoule to realize the function of testing the gas in the anaerobic culture bag, effectively improving the anaerobic culture bag anaerobic Oxygen test efficiency.

Description

technical field [0001] The invention belongs to the field of containers, in particular to an anaerobic culture bag with an anaerobic indicator. Background technique [0002] The cultivation of anaerobic bacteria not only requires the culture gas environment to be anaerobic, but also requires that the medium does not form peroxides that are harmful to anaerobic bacteria. During the production and storage of the medium, an anaerobic environment and suitable humidity should be maintained. At present, the prepared anaerobic medium is packaged and stored in air-permeable and water-permeable materials. When the air humidity is too high, water seeps in, the humidity of the culture gene is too high, and mold grows and becomes invalid; when the air humidity is too low, the water is lost, and the culture gene is too dry to fail; moreover, due to the infiltration of oxygen, the time is long, the oxygen cultivation The base forms peroxides that are harmful to anaerobic bacteria and mak...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/00
CPCC12M23/14C12M29/20C12M41/40
Inventor 蔡祥胜杨小蓉陈林珍王明珠黄演婷陈兴露卢汉威徐晓松卢景辉马晓桂李瑞莹官煜彬
Owner 广东药科大学附属第一医院
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