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Paraquat quick detoxifying liquid and method

A paraquat and fast technology, which is applied in the field of paraquat rapid detoxification solution, can solve the problems such as difficult to meet fast detoxification, unsatisfactory fast detoxification, slow response, etc., and achieves the effect of low cost, simple preparation method and detoxification

Active Publication Date: 2016-09-21
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Previous studies have shown that paraquat may be oxidatively degraded as an electron donor in the Fe(Ⅲ) / humus reduction process. This process reacts slowly and is more suitable for slow detoxification and restoration of soil, but it is difficult to meet the needs of rapid detoxification, especially It cannot meet the needs of rapid detoxification of paraquat poisoning in animals or humans

Method used

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  • Paraquat quick detoxifying liquid and method
  • Paraquat quick detoxifying liquid and method
  • Paraquat quick detoxifying liquid and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Paraquat antidote ( 1 ) preparation

[0038] Using strict aseptic operation, add Fe(III) humus-reducing bacteria (Kukia rosea) to the 100mL vial Kocuria rosea HN01, strain preservation number CGMCC No.5810) bacterial suspension, anthraquinone (9,10-anthraquinone-2,6-disulfonic acid, referred to as AQDS), sucrose, liquid medium, phosphate buffer solution, make the culture medium The total volume is 100 mL, and the initial concentration of each reactant is: 10mmol / L anthraquinone, 10mmol / L sucrose, 0.4mol / L phosphate buffer solution (provides buffer capacity, pH=6.0); then use high-purity nitrogen to inflate for 20min , discharge the oxygen in the culture medium of the vial, cover the rubber cap immediately after inflating, pressurize the aluminum cap to seal, and culture at 30°C in the dark for 5 days; become.

[0039] Paraquat solution and antidote ( 1 )reaction

[0040] Prepare a paraquat solution with a concentration of 200mg / L (pH6) with paraquat mothe...

Embodiment 2

[0046] Paraquat antidote ( 2 ) preparation

[0047] Using strict aseptic operation, add Fe(III) humus-reducing bacteria (Pseudomonas flexiformis) in the 100mL vial Pseudomonas geniculata PQ01 strain preservation number CCTCC NO: M 2014107) bacterial suspension, anthraquinone (AQDS), sucrose, liquid medium, phosphate buffer solution, so that the total volume of the culture medium is 100mL, and the initial concentration of each reactant is: 10mmol / L Anthraquinone, 10mmol / L sucrose, 0.4mol / L phosphate buffer solution (provide buffer capacity, pH=6.0); then inflate with high-purity nitrogen for 20min to discharge oxygen in the culture medium of the vial, cover the rubber cap immediately after inflating and add Press the aluminum cover to seal, and culture at 30°C in the dark for 5 days; finally, the vial is autoclaved (115°C, 20min), and the detoxification solution is prepared.

[0048] Paraquat solution and antidote ( 2 )reaction

[0049] Prepare a paraquat solution ...

Embodiment 3

[0055] Paraquat antidote ( 3 ) preparation

[0056] Using strict aseptic operation, add Fe(III) humus-reducing bacteria ( Kocuria rosea HN01) bacterial suspension, anthraquinone (9,10-anthraquinone-2-carboxylic acid, referred to as AQC), sucrose, liquid medium, phosphate buffer solution, so that the total volume of the culture medium is 100 mL, and the initial The concentration is: 10mmol / L anthraquinone, 10mmol / L sucrose, 0.4mol / L phosphate buffer solution (provide buffer capacity, pH=6.0); then use high-purity nitrogen to inflate for 20min, discharge the oxygen in the culture medium of the vial, and inflate completely Immediately cover the rubber cap and pressurize the aluminum cap to seal it, and incubate at 30°C in the dark for 5 days; finally, autoclave the vial (115°C, 20min) to prepare the detoxification solution.

[0057] Paraquat solution and antidote ( 3 )reaction

[0058] Prepare a paraquat solution with a concentration of 200mg / L (pH6) with paraquat m...

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Abstract

The invention discloses paraquat quick detoxifying liquid and a method. A preparation is a mixture of performing the light shading anaerobic cultivation for anthraquinone, Fe(III) humus reducing bacteria and a culture medium thereof, an electron donor and a pH buffering agent until enough anthraquinone is reduced as hydroquinone. The preparation is contacted and reacted with paraquat solution to quickly form a needle-shaped solid; and the needle-shaped solid is not dissolved in strong acid and strong alkali, is not dissolved in water and common organic solvent, is incapable of being absorbed by humans or animals, is more stable in normal-temperature and normal-pressure properties, and realizes quick paraquat detoxification. The preparation is simple in preparation method and low in cost.

Description

technical field [0001] The invention relates to a detoxification liquid and a detoxification method, in particular to a paraquat rapid detoxification liquid and a method. Background technique [0002] Paraquat (1,1-dimethyl-4,4-pyridine cationic salt) is a fast-acting contact-killing herbicide, which has a strong destructive effect on most monocotyledonous and dicotyledonous plants, and is widely used for immunity. In agricultural and forestry production such as tillage planting, rapid crop rotation, and direct-seeding rice, the global consumption is second only to glyphosate, and it is the second largest herbicide, and there is currently no substitute. [0003] Paraquat remains in the soil for a long time with a half-life of up to 644 days, and has strong environmental toxicity, such as: interfering with soil enzyme activity, affecting the immune system of fish and frogs, inhibiting the microbial community structure in the soil, etc., the environment caused by long-term par...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02A62D101/26
CPCA62D3/02A62D2101/26
Inventor 武春媛李勤奋吴东明
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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