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Method for removing uranium in enriched ryegrass degradation raffinate by microorganisms under anaerobic condition

An anaerobic condition, ryegrass technology, applied in anaerobic digestion treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve problems such as release into the environment, ecological pollution, etc.

Inactive Publication Date: 2020-05-29
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The use of microorganisms to rapidly reduce the volume of enriched ryegrass has achieved good results. However, if the degraded residue and residue are not properly treated, they will be released into the environment for the second time, causing ecological pollution.

Method used

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  • Method for removing uranium in enriched ryegrass degradation raffinate by microorganisms under anaerobic condition
  • Method for removing uranium in enriched ryegrass degradation raffinate by microorganisms under anaerobic condition
  • Method for removing uranium in enriched ryegrass degradation raffinate by microorganisms under anaerobic condition

Examples

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Embodiment 1

[0027] A method for removing uranium in enriched ryegrass degradation residue by microorganisms under anaerobic conditions, comprising the following steps:

[0028] Step 1: Add 100 mL of enriched ryegrass degradation residue, sodium lactate, anthraquinone-2-sulfonate and inorganic salt medium into the culture container, then inoculate Shewanella putrefaciens, and pass pure nitrogen into the culture container for 5 minutes. And place the culture container in an anaerobic incubator, culture at 30°C for 48 hours, and then centrifuge at 5000rpm for 10 minutes; the anaerobic incubator is replaced with pure nitrogen three times before use; the sodium lactate is degraded in the enriched ryegrass The concentration in the raffinate is 20mmol·L -1 ; The concentration of the anthraquinone-2-sulfonate in enriched ryegrass degradation raffinate is 2mmol L -1 ; The addition of the inorganic salt medium is 1 time of the volume of the enriched ryegrass degradation raffinate; the inoculation ...

Embodiment 2

[0031] A method for removing uranium in enriched ryegrass degradation residue by microorganisms under anaerobic conditions, comprising the following steps:

[0032] Step 1: Add 100 mL of enriched ryegrass degradation residue, sodium lactate, anthraquinone-2-sulfonate and inorganic salt medium into the culture container, then inoculate Shewanella putrefaciens, and pass pure nitrogen into the culture container for 5 minutes. And place the culture container in an anaerobic incubator, culture at 30°C for 48 hours, and then centrifuge at 5000rpm for 10 minutes; the anaerobic incubator is replaced with pure nitrogen three times before use; the sodium lactate is degraded in the enriched ryegrass The concentration in the raffinate is 20mmol·L -1 ; The concentration of the anthraquinone-2-sulfonate in enriched ryegrass degradation raffinate is 2mmol L -1 ; The addition of the inorganic salt medium is 1 time of the volume of the enriched ryegrass degradation raffinate; the inoculation ...

Embodiment 3

[0035] A method for removing uranium in enriched ryegrass degradation residue by microorganisms under anaerobic conditions, comprising the following steps:

[0036] Step 1: Add 100 mL of enriched ryegrass degradation residue, sodium formate, anthraquinone-2-sulfonate and inorganic salt medium into the culture container, then inoculate Shewanella putrefaciens, and pass pure nitrogen into the culture container for 5 minutes. And place the culture container in an anaerobic incubator, culture at 30°C for 48h, and then centrifuge at 5000rpm for 10min; the anaerobic incubator is replaced with pure nitrogen three times before use; the sodium formate is degraded in the enriched ryegrass The concentration in the raffinate is 20mmol·L -1 ; The concentration of the anthraquinone-2-sulfonate in enriched ryegrass degradation raffinate is 3mmol L -1 ; The addition of the inorganic salt medium is 0.5 times the volume of the enriched ryegrass degradation raffinate; the inoculum size of the d...

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Abstract

The invention discloses a method for removing uranium in enriched ryegrass degradation raffinate of by microorganisms under an anaerobic condition. The method comprises the following steps: adding enriched ryegrass degradation raffinate, an electron donor, anthraquinone-2-sulfonate and an inorganic salt culture medium into a culture container, then inoculating microorganisms, introducing pure nitrogen into the culture container, putting the culture container into an anaerobic incubator, carrying out static culture, and then carrying out centrifuging; before the anaerobic incubator is used, using pure nitrogen for gas replacement; adding FeCl2 into the centrifuged waste liquid, oscillating the waste liquid, and then centrifuging the waste liquid; adding an alkaline substance into the centrifuged waste liquid, adjusting the pH value to 11, and oscillating the waste liquid; and removing the uranium in the enriched ryegrass degradation raffinate. According to the method, uranium in the enriched ryegrass raffinate is removed through microorganisms combined with a chemical method, uranium and liquid are separated, the waste liquid meets the discharge requirement, and the removal efficiency of U (VI) in the enriched ryegrass raffinate reaches 99.90% or above.

Description

technical field [0001] The invention belongs to the field of microbial treatment of wastewater, and in particular relates to a method for removing uranium from enriched ryegrass degradation residue by microorganisms under anaerobic conditions. Background technique [0002] Phytoremediation is a bioremediation method that has attracted widespread attention at home and abroad in recent years. Phytoremediation technology is a technology that uses plants with high accumulation of heavy metals to adsorb heavy metals in soil and water. At present, many uranium-enriching plants have been screened out, but if these uranium-enriching plants are not properly treated, the heavy metals in their bodies will be released again, which will cause a second pollution to the environment, so these How to deal with heavy metal-enriched plants is a problem that needs to be dealt with emphatically. Biodegradation, as a green and economical disposal method of heavy metal-enriched biomass, has been ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/28C02F101/00C02F101/20C02F103/26
CPCC02F3/34C02F3/348C02F3/28C02F2101/006C02F2101/20C02F2103/26
Inventor 陈晓明田甲罗学刚敬路淮王丹陈浩
Owner SOUTHWEAT UNIV OF SCI & TECH
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