Anaerobic incubator capable of accurately controlling oxygen concentration and culture method based on anaerobic incubator

Abstract

The invention discloses an anaerobic incubator capable of accurately controlling oxygen concentration and a culture method based on the anaerobic incubator, and belongs to the field of biological devices. The incubator comprises an incubator body, an air collection device and a gas separation device, the incubator body comprises a culture bin and an equipment bin which is separated from the culture bin in a sealed mode, the air collection device is arranged in the equipment bin, the end of the air collection device is communicated with the culture bin and used for collecting air in the culture bin, and one end of the gas separation device is communicated with the air collection device; the other end is communicated with the culture bin. Air in the culture bin is collected into the gas separation device through the air collection device, then oxygen in the air is separated out through the gas separation device, and oxygen-free gas in which the oxygen is separated out is discharged into the culture bin, so that it can be guaranteed that the oxygen in the gas in the culture bin is fully separated out; further, the oxygen concentration in the culture bin is accurately controlled.

Description

Technical field [0001] The present invention relates to the field of biological devices, in particular to an anaerobic incubator that can accurately control the oxygen concentration and a culture method based on its culture. Background technique [0002] Anaharobacteria culture refers to bacteria cultured in an oxygen-free environment. In order to cultivate and store anaerobic bacteria, anaerobic bacterium is usually placed in an anaerobic incubator capable of controlling the oxygen concentration in a predetermined range. And the oxygen control method of the existing anaerobic incubator is to put the reactant or reaction liquid in the box before use, and the oxygen in the box is consumed, producing an oxygen-free environment, and the method is present in the presence of a reactant or reaction liquid. Or liquid, there is no uniform mixing with the gas in the box, so that oxygen in the box cannot be accurately controlled within a predetermined range, so there is a need to provide a...

AI Technical Summary

Problems solved by technology

[0002] Anaerobic culture refers to bacteria cultured in an anaerobic environment. In order to cultivate and preserve anaerobic bacteria, anaerobic bacteria are usually placed in an anaerobic incubator that can control the oxygen concentration in the box within a predetermined range. , and the oxygen control method of the existing anaerobic incubator is to put the reactant or the reaction solution into the box before use and then consume the oxygen in the box to produce an anaerobic environment. In this method, the reactant or the reaction solution is a solid Or liquid, there is a problem that it cannot be fully mixed with the gas in the box, so that the oxygen in the box cannot be accurately controlled within a predetermined range, so it is necessary to provide an anaerobic incubator that can accurately control the oxygen concentration

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