38results about How to "One-step synthesis" patented technology

The invention discloses a saccharomyces cerevisiae recombinant bacterium. According to the saccharomyces cerevisiae recombinant bacterium, an RgTAL gene, an HpaB gene and an HpaC gene are integrated in a genome. The invention further discloses a construction method and application of the saccharomyces cerevisiae recombinant bacteria. The invention finally discloses a production method of caffeic acid. According to the invention, three exogenous genes required by caffeic acid synthesis are integrated into saccharomyces cerevisiae chromosomes; total synthesis from glucose to caffeic acid is achieved through knockout of competitive pathway genes, elimination of feedback inhibition steps and overexpression of rate-limiting enzymes, the shake flask yield reaches about 760 mg/L, the shake flaskyield is the highest level of the yeast yield reported at present, and a new method is provided for industrial production of caffeic acid.

The invention provides a nitrogen-doped graphene and nitrogen-doped nano tin dioxide composite material and a preparation method and application thereof, and the preparation method comprises the following steps: respectively preparing graphene, a tin salt and a nitrogen-containing compound serving as raw materials into solutions; and carrying out mixing, heat treatment, heat preservation and drying to obtain the composite material. In the composite material, the mass percentage ratio of the nitrogen-doped graphene to the nitrogen-doped nano tin dioxide is 5%: 95%-30%: 70%. The crystal grain size of the nitrogen-doped nano tin dioxide is 1-5 nm, the Nitrogen-doped nano tin dioxide is uniformly loaded on the nitrogen-doped graphene. The total nitrogen doping amount of the nitrogen-doped graphene and nitrogen-doped nano tin dioxide composite material is 0.5%-1% of atomic ratio, and the specific surface area is 300-450m<2>/g. By use of the composite material disclosed by the invention, theelectron and lithium ion transmission speed can be effectively improved; the composite material can be used as a negative electrode material of a lithium ion battery; and the method has the advantages of few process steps, easiness in control, high repeatability and high product yield, thereby being beneficial to large-scale popularization.

The invention relates to a method for preparing bis(hydroxyethyl) bisphenol A ether. The method comprises the following steps: (1) washing a high-pressure reaction kettle with distilled water for several times, and cooling to room temperature for later use; (2) adding bisphenol A and a compound catalyst, vacuumizing to replace air, closing vacuum and beginning to warm when the vacuum degree is greater than or equal to -0.096MPa; (3) heating to 160 DEG C, carrying out vacuum degassing for 10 minutes, lastingly adding ethylene oxide, controlling the reaction temperature to be 150-175 DEG C and the pressure in the reaction kettle to be -0.05MPa to 0.5MPa, carrying out heat preservation after adding is ended, and further reacting until the pressure is not lowered more; and (4) cooling to 100 DEG C after reaction is ended, and carrying out vacuum degassing for 20 minutes, adding a certain amount of glacial acetic acid for neutralizing, cooling to 80 DEG C and emptying to obtain the finished product. The content of bis(hydroxyethyl) bisphenol A ether is greater than or equal to 95.0%; the content of mono-hydroxyethyl bisphenol A ether is smaller than or equal to 0.05%; the residual bisphenol A is smaller than or equal to 3ppm; the hydroxyl value is 350-355mgKOH/g; and the color and luster are 0-30# (Pt-Co unit).

The invention belongs to the technical field of lithium ion battery electrode materials, and discloses a metal formate/carbon nano tube lithium ion battery cathode material and a preparing method thereof. The lithium ion battery cathode material is prepared from metal nitrate, formic acid, N,N-dimethylformamide and acidized carbon nano tubes, wherein the metal nitrate includes nickel nitrate, cobalt nitrate, zinc nitrate and manganous nitrate, the molar ratio of the metal nitrate to formic acid is 1:5-1:8, and the mass of the acidized carbon nano tubes accounts for 10-40% that of the metal nitrate. The preparing method adopts a solvothermal method, conductivity of the obtained metal formate/carbon nano tube lithium ion battery cathode material is remarkably improved by means of effective cladding of the carbon nano tubes, the material has high specific capacity and circulation stability, respective advantages of the carbon nano tubes and a metal-organic framework material can be brought into play, and the material is an ideal lithium ion battery cathode material. The preparing method is simple in preparation technology and convenient and easy to operate.

The invention discloses a method for one-step controllable preparation of a uniform porous nitrogen-doped carbon material. Organic sugar and urea serve as raw materials, and the organic sugar and theurea are melted in proportion to form a homogeneous system; drying is carried out to preliminarily and rapidly remove a residual solvent; high-temperature treatment is carried out, so that the organicsugar is polymerized and carbonized at a high temperature, the urea is decomposed, and one-step synthesis of the porous nitrogen-doped carbon material is achieved. The raw materials involved in the method are cheap and easy to obtain, and the synthesis process is simple and environmentally friendly; the obtained carbon material is provided with graded pores including micropores and mesopores, controllable adjustment of the nitrogen doping amount, the porosity and the specific surface area can be realized, and the material has excellent optical, mechanical, electronic and energy storage capacity and has good application prospects in the fields of gas adsorption, adsorption of sulfur-containing and nitrogen-containing substances in oil, electrode materials, catalyst carriers and the like.

The invention discloses a preparation method and application of an MIL-125 (Ti) catalyst, and the preparation method comprises the following steps: mixing an N, N-dimethylformamide solution and a methanol solution to obtain a mixed solvent; dissolving an organic ligand in the mixed solvent, and uniformly stirring to obtain a mixed solution; adding tetrabutyl titanate and organic acid into the mixed solution, carrying out ultrasonic treatment, transferring into a polytetrafluoroethylene crystallization kettle, carrying out hydrothermal treatment, and carrying out centrifugal separation to obtain a crystallization product; and cleaning the obtained crystallization product, and carrying out vacuum drying to obtain the MIL-125 (Ti) catalyst. The MIL-125 (Ti) catalyst disclosed by the invention can be synthesized in one step, the crystal size of the catalyst can be effectively controlled through the addition amount of organic acid, and thiophene sulfide can be catalytically oxidized at room temperature, so that more efficient desulfurization is realized; the preparation method of the catalyst is simple, and the catalyst has a good application prospect in the aspect of oil product desulfurization.

The invention relates to a method for synthesizing a preservative and antistaling agent, and in particular relates to a method for synthesizing a sodium diacetate crystal in one step. The following raw materials are adopted in percent by weight: 70.4-72.4% of glacial acetic acid, 10.8-12.0% of ionic membrane method caustic soda flake and 16.5-18.6% of anhydrous sodium carbonate. The synthesis steps are as follows: (1) mixing the ionic membrane method caustic soda flake with the anhydrous sodium carbonate, slowly adding the glacial acetic acid and then naturally heating, controlling total reaction time to be 30-50 minutes, and controlling maximum reaction temperature to be 65-85 DEG C; and (2) cooling the temperature below 50-60 DEG C after the reaction is completed, and discharging. By applying the method provided by the invention, solid-liquid separation equipment, drying equipment and a reaction heating device are eliminated, a sodium diacetate crystal one-step synthesis method is realized, process is simple and safe, energy is saved, production cost can be greatly reduced, yield of the product reaches up to more than 97%, product quality is easy to control, and the standard of FAO/WHO (Food and Agriculture Organization/World Health Organization) is met.

The invention relates to a preparation method for a Bi2O2CO3-Bi2WO6 composite photocatalyst. The preparation method comprises the following steps: step 1, adding Bi(NO3)3.5H2O and tungstate sequentially into a methanol solution, and performing mechanical stirring to obtain a mixed solution, wherein the molar ratio of the Bi(NO3)3.5H2O to the tungstate is (1-10):1; step 2, performing heat preservation on the mixed solution at the intensity of pressure of 3Mpa or more and at 150-200 DEG C for 3-12 hours for reaction; and step 3, after the reaction ends, performing natural cooling to room temperature, and performing centrifugation, water washing and drying at room temperature on a product to obtain the Bi2O2CO3-Bi2WO6 composite photocatalyst. The preparation method has the characteristics ofhigh light quantum efficiency and high visible-light activity.

The invention provides a preparation method of an amorphous alloy additive. The preparation method comprises the following steps of heating a metal material by using pulse laser of which the frequencyis higher than the plasma frequency of the processed metal material until the metal material is molten, and quickly cooling the molten metal material by limiting the pulse width of the pulse laser tobe 10fs-1ms. According to the preparation method, a amorphous alloy three-dimensional part can be manufactured, generation of nano-crystalline grains in the amorphous alloy can be avoided, and applicable amorphous alloy components are remarkably larger than those of an amorphous alloy component area covered in the prior art.

The invention provides a method for carrying out a trans-Micheal addition reaction by taking a compound with an aurone skeleton as a receptor, and belongs to the technical field of chemical synthesis.According to the method, a reaction can be carried out under the conditions that Sc(OTf) 3 is used as a catalyst, the temperature is 80 DEG C and dichloromethane is used as a solvent. According to the method provided by the invention, the alpha-functionalized trans-Michael addition product of oxadiene is obtained through a negative hydrogen migration/aromatization/dearomatization/cyclization reaction by taking a carbonyl group conjugated with an aurone skeleton compound and a double bond as a receptor for Michael addition under the catalysis of Lewis acid. The method is simple and practical to operate, efficient and convenient in reaction, wide in substrate applicability and extremely high in atom economy.

The invention discloses yeast engineering bacteria for fermentation production of chondroitin sulfate and an application of the yeast engineering bacteria, and belongs to the technical field of bioengineering. Pichia pastoris GS115 and S.cerevisiae CEN.PK2-1C are used as starting strains by utilizing a synthetic biological technology and a genetic engineering means, and chondroitin sulfate synthetic pathway related genes including genes kfoC and kfoA from escherichia coli K4, chondroitin sulfate transferase genes C4ST and C6ST from mice and UDP-glucose dehydrogenase genes tuaD from bacillus subtilis are heterologously expressed in cells. Production strains for synthesizing chondroitin sulfate A (CSA), chondroitin sulfate C (CSC) and chondroitin sulfate E (CSE) are obtained from ATP sulfatase gene MET13 of S.cerevisiae. According to the invention, chondroitin sulfate with different configurations is synthesized by utilizing a microbial fermentation carbon source for the first time.

The invention discloses a method for synthesizing 1,5-diaminonaphthalene by one step with naphthalene. The method is characterized in that in a mixed solvent of glacial acetic acid and water, naphthalene and hydroxylammonium salts carry out one-step reaction in the presence of catalysts, namely metavanadate and/or vanadium oxide, to generate 1,5-diaminonaphthalene. The method is low in cost, simple in process, short in flow and mild in reaction conditions, is environment-friendly and meets the industrial production requirements.

The invention discloses a method for synthesizing 1,5-diaminonaphthalene with 1-naphthylamine. The method is characterized in that in a mixed solvent of glacial acetic acid and water, 1-naphthylamine and hydroxylammonium salts carry out one-step reaction in the presence of catalysts, namely metavanadate and/or vanadium oxide, to generate 1,5-diaminonaphthalene. The method is low in cost, simple in process, short in flow and mild in reaction conditions, is environment-friendly and meets the industrial production requirements.

The invention relates to a preparation method of bis-hydroxypropyl neopentyl glycol ether and belongs to the technical field of organic compound synthesis. According to the technical scheme of the preparation method of bis-hydroxypropyl neopentyl glycol ether, neopentyl glycol is adopted as a raw material, and a product of bis-hydroxypropyl neopentyl glycol ether is adopted as a solvent. A 3-(4-methoxyphenyl) phosphine catalyst is added. After that, propylene oxide is adopted as a chain extender for the synthesis of bis-hydroxypropyl neopentyl glycol ether. The method is applied to the synthesis of bis-hydroxypropyl neopentyl glycol ether and has the advantages of high product content, low neopentyl glycol content, low content of allyl alcohol as a by-product, stable quality and the like.

The invention relates to a universal preparation method of a nitrogen and phosphorus co-doped carbon-loaded transition metal phosphide. The method comprises the following steps: taking melamine polyphosphate as a carbon source, a nitrogen source and a phosphorus source, compounding the melamine polyphosphate with a transition metal raw material, and calcining at high temperature to obtain the nitrogen-phosphorus co-doped carbon-loaded transition metal phosphide. In the preparation method provided by the invention, melamine polyphosphate is pyrolyzed to obtain a nitrogen-phosphorus co-doped carbon material while a transition metal raw material is converted into a corresponding phosphide, so that one-step synthesis of the carbon-loaded transition metal phosphide is realized. Compared with the prior art, the preparation method has the advantages that complicated precursor preparation and phosphating treatment processes are not needed, and the preparation process of the carbon material loaded transition metal phosphide is remarkably simplified. The method provided by the invention is low in raw material cost, high in universality and simple to operate, can realize large-scale preparation of the carbon-loaded transition metal phosphide, and has important application value.