Vascular tissue engineering reactor having vas stretch and pulsating flow pouring functions

A vascular tissue and reactor technology, applied in specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, tissue cell/virus culture device, etc., can solve the problem of not carefully considering flow impedance, compliance Flow inertia, inability to provide periodic axial stretching of vascular tissue at the same time, inability to provide a near-physiological flow environment, etc., to achieve the effect of good mass transfer performance and good controllability

Active Publication Date: 2009-02-25
BEIHANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In summary, there are relatively large defects in the existing vascular tissue engineering reactors: the first is that factors such as flow resistance, compliance (elasticity of the vessel wall and buffering capacity), resistance, flow inertia, etc. The simple simulation of the dynamic environment cannot provide a near-physiological flow environment similar to the pulsating fl

Method used

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  • Vascular tissue engineering reactor having vas stretch and pulsating flow pouring functions
  • Vascular tissue engineering reactor having vas stretch and pulsating flow pouring functions
  • Vascular tissue engineering reactor having vas stretch and pulsating flow pouring functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1 Perfusion stretching in pulsatile flow

[0087] 1. According to figure 2 The intravascular perfusion circuit is connected as shown, wherein the intravascular perfusion liquid driving device is the pulse source;

[0088] 2. Disinfect the reactor, the disinfection conditions are: 130°C, 3 atmospheres, 1 hour;

[0089] 3. Install the tissue engineered blood vessel to be cultured on the upstream and downstream pipelines of the intravascular perfusion circuit in the vascular tissue culture cavity under sterile conditions, wherein the inner diameter of the tissue engineered blood vessel is 6 mm, the length is 20 cm, and PLGA;

[0090] 4. According to figure 2 The connected vascular tissue stretching device is shown, wherein the vascular tissue stretching motor is a Haydn 57000 series linear stepping motor;

[0091] 5. Prepare the medium according to the culture requirements, inject the sterile medium into the intravascular perfusion storage bottle;

[0092] 6....

Embodiment

[0097] Example 2 Pulsating flow, perfusion, stretching, and internal rotation

[0098] 1. According to image 3 The intravascular perfusion circuit is connected as shown, wherein the intravascular perfusion liquid driving device is the pulse source;

[0099]2. Disinfect the reactor, the disinfection conditions are: 130°C, 3 atmospheres, 1 hour;

[0100] 3. Install the tissue-engineered blood vessel to be cultured on the liquid distributor upstream and downstream of the vascular tissue culture chamber in a sterile state, wherein the inner diameter of the tissue-engineered blood vessel is 4 mm, the length is 10 cm, and PLGA;

[0101] 4. According to image 3 The vascular tissue rotation device shown is connected, and the vascular tissue rotation motor is a Haydn 57000 series stepping motor;

[0102] 5. According to image 3 The pressure sensor connected to the inlet and outlet of the vascular tissue culture chamber in the intravascular perfusion circuit and the signal detect...

Embodiment 3

[0112] Example 3 Stretching with pulsating flow inner perfusion and outer perfusion

[0113] 1. According to Figure 4 Connect the intravascular and extravascular perfusion circuits as shown, wherein the intravascular perfusion liquid driving device is the pulsation source, and the extravascular perfusion liquid driving device is a peristaltic pump (Cole-Parmer Company, Masterflex series);

[0114] 2. Disinfect the reactor, the disinfection conditions are: 130°C, 3 atmospheres, 1 hour;

[0115] 3. Install the tissue-engineered blood vessel to be cultured on the liquid distributor upstream and downstream of the vascular tissue culture chamber in a sterile state, wherein the inner diameter of the tissue-engineered blood vessel is 6 mm, the length is 20 cm, and PLGA;

[0116] 4. According to Figure 4 The pressure sensor and signal detection device connected to the inlet and outlet of the vascular tissue culture chamber in the intravascular and external perfusion circuits are s...

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Abstract

The invention relates to a vascular tissue engineering reactor which is characterized by comprising intravascular perfusion liquid storage bottles (101) which are connected with each other in sequence by pipelines, a pulsation source (102) which is taken as an intravascular perfusion liquid driving device, a culture cavity inlet pipeline (202) of an intravascular perfusion circuit, and a vascular tissue culture cavity (107), wherein, one end of an inlet pipeline (104) of the culture cavity enters the vascular tissue culture cavity (107), and is connected with one end of a vascular tissue (108) to be cultured so as to cause the culture solution from the perfusion liquid storage bottle (101) to flow into the vascular tissue (108). The reactor further comprises a culture cavity outlet pipeline (204) of the intravascular perfusion circuit, one end of the culture cavity outlet pipeline (204) enters the vascular tissue culture cavity (107), and is connected with the other end of the vascular tissue (108) to be cultured so as to export the culture solution from the inner part of the vascular tissue (108).

Description

technical field [0001] The invention relates to the fields of cell culture and tissue engineering, and more specifically relates to a vascular tissue engineering reactor with the functions of blood vessel stretching and pulsation. Background technique [0002] So far, autografts, allogeneic blood vessels, xenograft blood vessels, and artificially synthesized blood vessels have not been able to become ideal arterial blood vessel substitutes (especially blood vessels with a caliber of less than 6mm), but in recent years, arterial blood vessel tissue engineering bioreactors have been used to construct And research into tissue-engineered arterial vessels brings hope to the field. At present, the research content of bioreactors for arterial tissue engineering mainly includes: [0003] 1. Realize and control the uniform and high-density seeding of cells in the scaffold for vascular tissue: recent studies have found that direct perfusion bioreactors are superior to static, rotary ...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/36
CPCC12M29/10C12M29/12C12M21/08C12M27/10
Inventor 樊瑜波黄学进李晋川邹远文贡向辉
Owner BEIHANG UNIV
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