Vascular tissue engineering reactor having vas stretch and pulsating flow pouring functions

Abstract

The invention relates to a vascular tissue engineering reactor which is characterized by comprising intravascular perfusion liquid storage bottles (101) which are connected with each other in sequence by pipelines, a pulsation source (102) which is taken as an intravascular perfusion liquid driving device, a culture cavity inlet pipeline (202) of an intravascular perfusion circuit, and a vascular tissue culture cavity (107), wherein, one end of an inlet pipeline (104) of the culture cavity enters the vascular tissue culture cavity (107), and is connected with one end of a vascular tissue (108) to be cultured so as to cause the culture solution from the perfusion liquid storage bottle (101) to flow into the vascular tissue (108). The reactor further comprises a culture cavity outlet pipeline (204) of the intravascular perfusion circuit, one end of the culture cavity outlet pipeline (204) enters the vascular tissue culture cavity (107), and is connected with the other end of the vascular tissue (108) to be cultured so as to export the culture solution from the inner part of the vascular tissue (108).

Description

technical field [0001] The invention relates to the fields of cell culture and tissue engineering, and more specifically relates to a vascular tissue engineering reactor with the functions of blood vessel stretching and pulsation. Background technique [0002] So far, autografts, allogeneic blood vessels, xenograft blood vessels, and artificially synthesized blood vessels have not been able to become ideal arterial blood vessel substitutes (especially blood vessels with a caliber of less than 6mm), but in recent years, arterial blood vessel tissue engineering bioreactors have been used to construct And research into tissue-engineered arterial vessels brings hope to the field. At present, the research content of bioreactors for arterial tissue engineering mainly includes: [0003] 1. Realize and control the uniform and high-density seeding of cells in the scaffold for vascular tissue: recent studies have found that direct perfusion bioreactors are superior to static, rotary ...

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Problems solved by technology

[0012] In summary, there are relatively large defects in the existing vascular tissue engineering reactors: the first is that factors such as flow resistance, compliance (elasticity of the vessel wall and buffering capacity), resistance, flow inertia, etc. The simple simulation of the dynamic environment cannot provide a near-physiological flow environment similar to the pulsating flow state of blood in the artery; secondly, it cannot provide the vascular tissue with mechanical forces such as periodic axial stretching, radial stretching, and twisting that approximate the physiological state at the same time The third is that most of them use the method of no wall rotation, which needs to be improved in terms of uniform cell, high-density seeding and mass transfer

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