Process for moderate-temperature self-assembly preparation of nano starch from enzymolysis short amylose

A technology of short amylose and nano-starch, applied in fermentation and other directions, can solve the problems of low yield of nano-starch, long preparation time, large energy consumption, etc., and achieve the effects of environmental protection, simple operation, and increased yield.

Active Publication Date: 2015-07-29
QINGDAO AGRI UNIV
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Problems solved by technology

In the process of preparing nanoparticles by the miniemulsion method, the particle size of the prepared nanoparticles is controllable, but a large amount of organic solvents and surfactants are required
[0007] In summary, these methods for preparing nano starch have disadvantages such as low yield, long preparation time, and high energy consumption.

Method used

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  • Process for moderate-temperature self-assembly preparation of nano starch from enzymolysis short amylose
  • Process for moderate-temperature self-assembly preparation of nano starch from enzymolysis short amylose
  • Process for moderate-temperature self-assembly preparation of nano starch from enzymolysis short amylose

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preparation example Construction

[0050] (2) Preparation of starch aqueous solution: take degreased starch, add 100ml distilled water, and prepare starch milk solution with a concentration of 1-30%;

[0051] (3) Gelatinization: use citric acid buffer to adjust the pH of the starch solution to 4.6, and then heat it in a water bath for 30 minutes under boiling water bath conditions. During the gelatinization process, it is necessary to stir the starch continuously with a mixer so that the gelatinization is complete;

[0052](4) Enzymolysis: cool to about 58°C and add, add 4u / g pullulanase, the reaction conditions are to adjust the pH to 4.6, and react for 8 hours in a constant temperature water bath at 58°C; then use the degreased starch solution with 1:4 ethanol precipitation;

[0053] (5) Centrifuge the precipitated starch solution with a centrifuge to remove the supernatant to obtain a precipitate, and then wash it with water for 2-3 times. Freeze-drying obtains short amylopectin;

[0054] (6) Insulate the ...

Embodiment 1

[0072] A process for preparing nano-starch by enzymatically hydrolyzing short amylose at medium temperature self-assembly, the preparation method of which is as follows:

[0073] (1) Take by weighing 15g waxy cornstarch after degreasing, add 100ml distilled water, the starch milk solution of the 15% concentration of preparation; Regulate the pH of starch solution with citric acid buffer solution to be 4.6 and then heat in water bath under boiling water bath condition for 30min, adjust When the pH reaches 4.6, add pullulanase and react in a constant temperature water bath at 58°C for 8 hours; then precipitate the defatted starch solution with 1:4 absolute ethanol; remove the precipitated starch solution with a centrifuge The supernatant was obtained as a precipitate. Then wash with water 2-3 times. Freeze-drying with a lyophilizer to obtain short amylopectin; incubating the obtained short amylose at a medium temperature of 50° C. for 24 hours, and centrifuging to remove the su...

Embodiment 2

[0075] A process for preparing nano-starch by enzymatically hydrolyzing short amylose starch and self-assembly at medium temperature. The preparation method is as follows: Weigh 10 g of waxy corn starch after defatting, add 100 ml of distilled water, and prepare a 10% starch milk solution; buffer with citric acid After adjusting the pH of the starch solution to 4.6, heat the starch solution in a boiling water bath for 30 minutes, adjust the pH to 4.6, add pullulanase, and react for 8 hours in a constant temperature water bath at 58°C; then degrease the starch solution with 1: 4 of absolute ethanol precipitation; the precipitated starch solution was centrifuged with a centrifuge to remove the supernatant to obtain a precipitate. Then wash with water 2-3 times. Freeze-drying with a lyophilizer to obtain short amylopectin; incubating the obtained short amylose at a medium temperature of 30° C. for 12 hours, and centrifuging to remove the supernatant to obtain a precipitate. Quic...

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Abstract

The invention discloses a process for moderate-temperature self-assembly preparation of nano starch from enzymolysis short amylose. The process particularly comprises the following steps: (1) preparing a buffer solution; (2) preparing a starch water solution; (3) past-forming; (4) enzymolyzing, and then using anhydrous ethanol to precipitate the degreased starch solution; (5) preparing short amylose; (6) preparing nano starch. According to the process, enzyme hydrolysis starch is adopted to prepare nano starch at 30-70 DEG C in an anabiosis manner, the nano starch prepared by the method is high in productivity, low in cost, safer and more environment-friendly, and can serve as embedding materials of medicines, active ingredients and the like, and the application field of the process in the food industry is widened.

Description

technical field [0001] The invention belongs to the technical field of starch nanoparticle preparation, and in particular relates to a process for preparing nano-starch by enzymatically hydrolyzing short amylose starch and self-assembling at medium temperature, in particular to a process for preparing nano-starch by combining enzymatically hydrolyzed starch with self-assembly equipment at 50°C. Background technique [0002] Starch is widely distributed in nature and is the main form of carbohydrate storage. It is divided into two types: amylose and amylopectin. Starch exists in leaves, stems, roots, fruits and other organs of most higher plants. It is an important energy substance and industrial raw material, and is widely used in papermaking, food, chemical and other fields. [0003] Starch nanoparticles are a kind of nano-sized polysaccharide crystals prepared from native starch, which exhibit many physical and chemical properties due to properties such as small size effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/12C08L3/12C12P19/16C12P19/04
Inventor 孙庆杰熊柳刘成珍秦洋姜岁岁刘珠珠
Owner QINGDAO AGRI UNIV
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