Caulis trachelospermi total lignans extractive, extraction method and medicine use of the extractive and active constituent thereof

A technology of total lignans and extracts, applied in the field of total lignans and its extraction, can solve the problems of unclear anti-inflammatory and analgesic active ingredients

Inactive Publication Date: 2007-02-28
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-inflammatory and analgesic active ingredients in it have been unclear. Up to now, there have been no reports on the total lignan...

Method used

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  • Caulis trachelospermi total lignans extractive, extraction method and medicine use of the extractive and active constituent thereof
  • Caulis trachelospermi total lignans extractive, extraction method and medicine use of the extractive and active constituent thereof
  • Caulis trachelospermi total lignans extractive, extraction method and medicine use of the extractive and active constituent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of total lignans extract from vine vine

[0031] Preparation

[0032] Caterpillaria chinensis (1kg) was reflux extracted three times with 95% ethanol (V:W=6:1), filtered through gauze, and concentrated under reduced pressure to obtain 187.5 g of the extract. Dissolve in 95% ethanol, add 282g of diatomite (crude paste: diatomite=1:1.5), mix well, evaporate to dryness, pulverize, add 536ml of petroleum ether to extract three times at room temperature, filter, and concentrate the filtrate to 9.177g of petroleum ether dissolved part . The filter residue was extracted twice with 95% ethanol under reflux. Suction filtration while hot, and the filtrate was concentrated to obtain 173.1 g of extract. Dissolve in 2L of 10% ethanol, centrifuge (3000 rpm, 10 min), take the supernatant and put it on an AB-8 macroporous resin column (column bed volume 2L, pre-equilibrated with 10% ethanol). First elute with 4L of 10% ethanol, and discard the eluate after recovering th...

Embodiment 2

[0034] Anti-inflammatory evaluation of the extract of Example 1 and its main active ingredients

[0035] Experimental Materials

[0036] Experimental animals: male Kunming mice, weighing 18-22 g; male Sprague-Dawley rats, weighing 140-180 g; all were purchased from and bred in the Experimental Animal Center of our hospital.

[0037] Tested sample: extract of Example 1; demethylorthoside (1), methanoside (2), demethylosiderin (3), podocarpus resinol (4), terrosidegenin (5) and Arctigenin (6) (separated by the inventors from the extract of Tractoria vine, and the purity measured by liquid phase normalization method is above 98%), celecoxib (manufactured by Pfizer).

[0038] Experimental equipment: hole punch, self-made equipment for measuring rat paw volume, etc.

[0039] Experimental reagents: croton oil and carrageenan were purchased from sigma company, and the others were of domestic analytical grade.

[0040] Experimental method and results:

[0041] Croton oil-induced ear...

Embodiment 3

[0057] Evaluation of the analgesic effect of the extract of Example 1

[0058] Experimental Materials

[0059] Experimental animals: Kunming mice, half male and half male, weighing 18-22 g, purchased from and bred in the Experimental Animal Center of our hospital.

[0060] Test samples: the extract of Example 1, indomethacin (purchased from Cayman Company).

[0061] Experimental equipment: 2L beaker, video camera, etc.

[0062] Reagents used in experiments: all domestic analytical pure.

[0063] Experimental method and results:

[0064] 40 mice were randomly divided into 5 groups, 8 in each group, administered by intragastric administration (ig) (fasting for 16 hours before administration) or intraperitoneal injection (ip) of 0.1ml, and the blank control group was given the same volume of water or normal saline. Half an hour later, intraperitoneally inject (ip) 0.2ml of 0.6% acetic acid solution, and after standing for 5 minutes, record the number of writhing times of eac...

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Abstract

The invention discloses a total lignans from caulis trachelospermi and extracting method, which is characterized by the following: possessing 30-90% lignans in the total lignans; fitting for preparing medicine to treat inflammation and or pain; making the lignan as inhibitor of epoxidised enzyme.

Description

field of invention [0001] The present invention relates to total lignans extracted from vine vine and its extraction method. The total lignans extract contains 30-90% by weight of lignans. The present invention also relates to the use of the extract and its effective lignans in the preparation of medicines for treating inflammation and / or pain and as cyclooxygenase (COX) inhibitors in the preparation of other diseases such as Use in drugs for tumors, thrombus, etc. Background technique [0002] Trachelospermum jasminoides (Lindl.) is the stem and leaf of Trachelospermum jasminoides (Lindl.), which is a commonly used traditional Chinese medicine recorded in the Chinese Pharmacopoeia. For the treatment of rheumatoid arthritis, joint pain, muscle pain, waist and knee pain, sore throat, vomiting blood, traumatic bleeding, etc. However, the anti-inflammatory and analgesic active ingredients in it have been unclear. Up to now, there have been no reports on the total lignan extra...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08C07B63/00C07D307/33A61K31/7048A61K31/365A61P29/00A61P35/00A61P7/02
Inventor 于能江赵毅民任凤霞
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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