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Use of erberry and its lignanoids compound in drug for osteoporosis

An anti-osteoporosis and lignan technology, applied in the direction of drug combination, bone diseases, pharmaceutical formulations, etc., can solve the problems of less research on the mechanism of action, complex traditional Chinese medicine ingredients, unclear index ingredients and active ingredients, etc.

Inactive Publication Date: 2005-07-06
CHINESE MEDICINE & NATURAL MEDICINE RES CENT SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although in vivo experiments have proved that traditional Chinese medicine is indeed effective in treating osteoporosis with fewer side effects, but due to the complex composition of traditional Chinese medicine, the index components and active ingredients are not clear, the research on the mechanism of action is rare, and the treatment target is difficult to confirm. It is currently difficult to be accepted by the international community.

Method used

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  • Use of erberry and its lignanoids compound in drug for osteoporosis
  • Use of erberry and its lignanoids compound in drug for osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] 30 kg of stems and branches of elderberry (Sambucus Williamsii Hance) were crushed and extracted twice under reflux with 8 times the amount of 60% ethanol for 2 hours each time. The extracts were combined, and the solvent was evaporated under reduced pressure to obtain 900 g of ethanol extract. For this extraction, pure ethanol and pure methanol can also be used instead of 60% ethanol.

[0011] 350 grams of ethanol extract are used in Example 2. 550 grams of ethanol extract was suspended in 4000 milliliters of water and extracted 3 times with equal volumes of chloroform, ethyl acetate and n-butanol, and the solvent was evaporated under reduced pressure to obtain 100 grams of chloroform extract and 30 grams of ethyl acetate extract. Butanol extract 70 grams, water extract 330 grams. Methyl acetate and butyl acetate can replace ethyl acetate, and isopropanol can replace n-butanol.

[0012] The aqueous suspension of ethanol extract can also be directly extracted with et...

Embodiment 2

[0015] The in vivo bioactivity test results of the elderberry ethanol extract obtained from Example 1 are described.

[0016] Three-month-old female SD rats were randomly divided into 5 groups, 4 groups with ovariectomy and 1 group with sham operation. The rats in the ovariectomized group (OVX) were anesthetized with ether and had their bilateral ovaries removed. The rats in the sham operation group (Sham) were also anesthetized with ether, but the ovaries were not removed, and only a little fat outside the ovary was cut off. One month after the operation, the ethanol extract of elderberry was dissolved in distilled water to prepare two concentrations of 600mg / ml and 300mg / ml, and the positive control drug 17β-estradiol was suspended in distilled water, and administered by intragastric administration respectively. The model control group and the sham operation group were given the same volume of distilled water. The administration continued for 3 months, and the body weight o...

Embodiment 3

[0084]Illustrate that the Elderberry chloroform extract obtained from Example 1, the ethyl acetate extract obtain and identify 12 lignan compounds through silica gel column chromatography, molecular sieve chromatography, ODS column chromatography and high performance liquid phase , 4 phenolic acid compounds (including their derivatives), new uses for the treatment of osteoporosis. emodin (emodin, compound 1), betulin (betulin, compound 2), betulinic acid (betulinic acid, compound 3), vanillin (vanillin, compound 5), coniferaldehyde (compound 7), (-)-lirioresinol C ( Compound 10), (-)-diapinoresinol (Compound 11), 1-(4-hydroxy-3-methoxyphenyl)-2-(4-hydroxy-3-methoxyphenyl)-1,3-propanodiol (Compound 12), guaiacylglycerol- β-O-4′-coniferyl ether (compound 13), (-)-erytheo-1-(4-hydroxy-3-methoxy--phenyl)-2-(4-hydroxypropyl-2-methoxyphenoxy)-1,3 -propanodiol (compound 15), (-)-threo-1-(4-hydroxy-3--methoxyphenyl)-2-(4-hydroxypropyl-2-methoxyphenoxy)-1,3-propanodiol (compound 16), ...

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Abstract

The invention relates to the use of Sambucus Williamsii Hance as osteoporosis resisting medicament, and the use of lignan compounds and phenolic acid compounds as osteoporosis resisting medicament, wherein the lignan compounds and phenolic acid compounds are prepared through the steps of ethanol heating backflow of Sambucus Williamsii Hance stems and branches, extracting ethanol extract, suspending in water and extracting with chloroform, acetic acid ethyl ester, and n-butyl alcohol, disintegrating with chemical means. The invention shows no side action of estrogen samples.

Description

1. Technical field [0001] The present invention relates to the use of folk herbal medicine elderberry (Sambucus Williamsii Hance) extract as anti-osteoporosis, and the application of lignan compounds and phenolic acid compounds isolated therefrom as anti-osteoporosis drugs. 2. Background technology [0002] Osteoporosis is a metabolic bone disease characterized by decreased bone mass and degraded bone tissue microenvironment, which leads to increased bone fragility, and patients are prone to fractures. It has been recognized as a serious public health problem in the past two decades . According to the statistics of the World Health Organization, in the United States, the annual cost of treating hip fractures caused by osteoporosis is 10 billion US dollars, which is equivalent to the cost of treating cardiovascular diseases and asthma [3]. According to the International Osteoporosis Foundation (International Osteoporosis Foundation), in the European Union, every 30 seconds t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61P19/10
Inventor 姚新生黄文秀王乃利杨序娟解芳张岩
Owner CHINESE MEDICINE & NATURAL MEDICINE RES CENT SHENZHEN
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