Antimicrobial agent screening method

A technology for antimicrobials, screening methods, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problem of removing evaporator cores, mold and bacteria unpleasant

Active Publication Date: 2016-11-16
HYUNDAI MOTOR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although various air fresheners are commercially available to remove this unpleasant smell, they do not actually remove the mold and bacteria growing on the evaporator core but only temporarily dilute the unpleasant smell
In addition, currently commercially available antimicrobial agents have not been developed to specifically act on specific molds or bacteria that multiply on the evaporator core but are used because of their antimicrobial effect on general pathogens

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Screening for dominant microorganisms that cause bad odors

[0069] 1. Preparation of old cars emitting bad smell and separation of air conditioning system

[0070] In order to identify the cause of bad smell in airtight environment such as vehicle interior, isolated from 10 old cars that gave off bad smell in different seasons (winter: February-March, summer: June-July) Air Conditioning System. Next, samples were taken from evaporator cores on which biofilms of microorganisms that cause bad odors in air conditioning systems were expected to form (Table 1).

[0071] Table 1

[0072]

[0073] 2. Preparation of Evaporator Core Samples

[0074] The evaporator wicks isolated from the bad smelling old cars 1-10 were stored at 4°C and sealed in polyethylene bags until the evaporator wick samples were used. To isolate and cultivate microorganisms, use sterile needle-nose pliers to take 5 g samples from random parts of each evaporator core, including the fro...

Embodiment 2

[0145] Example 2: Evaluation of antimicrobial activity of antimicrobial agents against screened malodorous microorganisms

[0146] 1. Experimental steps

[0147] The antimicrobial activity of the dominant microorganisms screened in Example 1 was evaluated with various commercially available antimicrobial agents. The antimicrobials tested were as follows:

[0148] Antimicrobial Agent A: fabric deodorant available from P&G Korea.

[0149] Antimicrobial Agent B: hand sanitizer purchased from Paru Corporation.

[0150] Antimicrobial C: A mass-produced antimicrobial containing 45-50% methanol, 1-5% chromium sulfate (CAS 10101-53-8), 1-5% bromine and water.

[0151] Antimicrobial agent D: cationic antimicrobial agent (Parkerizing, Japan).

[0152] Antimicrobial Agent E: Isothiazolinone-based antimicrobial agent containing methylisothiazolinone (CAS 26172-55-4), bronopol (CAS 52-51-7) and the like (Parkerizing, Japan).

[0153] Antimicrobial activity was evaluated as follows: ...

Embodiment 3

[0171] Example 3: Evaluation of the odor of evaporator cores freed from odor-causing microorganisms

[0172] In order to prepare the evaporator core in which the microorganisms causing bad smell were removed or separated, the odorless microorganisms excluding the microorganisms causing bad smell of Example 1 from the dominant strains inhabiting the evaporator core were combined on the aluminum Culturing was performed on-chip, where aluminum was the material of the evaporator core (Table 10, image 3 ).

[0173] Predominant species that formed colonies on the evaporator core but did not cause an unpleasant odor were selected as odorless microorganisms and cultured as follows.

[0174] 1) Inoculate the isolated odorless microorganisms into liquid R2A medium.

[0175] 2) Cultivate the microorganisms at a temperature of 28°C for 5-7 days.

[0176] 3) Prepare aluminum fins sterilized at 121° C. for 20 minutes under high pressure.

[0177] 4) Uniformly coat the surface of the ...

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Abstract

The present invention relates to a screening method of an antimicrobial agent that can control microbes which cause odor in an air conditioner, and a method for removing odor in an air conditioner. Odor-causing microbes in the air conditioner of the present invention can be used in the development of a novel antimicrobial agent or in the development of an odorant for blocking the odor by investigating the chemical properties of metabolites of the microbes. In addition, by creating an environment, in advance, where the microbes cannot inhabit the air conditioner, the present invention has diverse industrial utility in being used for fundamentally removing the cause of odor, and so on.

Description

technical field [0001] The present invention relates to methods for screening antimicrobial agents capable of controlling microorganisms that can cause bad odors in air conditioning systems and methods for removing bad smells in air conditioning systems. Background technique [0002] Clean air is considered an integral component of human health and well-being, and bad-smelling or polluted air prevents a pleasant environment. For example, unsatisfactory indoor air quality in airtight conditions is caused by two important factors. One is air pollutants produced by the structures (buildings or vehicles) that make up the airtight environment itself, and the other is odors from human activities or from materials brought in from the outside. [0003] Air conditioning systems for buildings, vehicles, trains, ships, airplanes, etc. refer to systems designed to reduce indoor temperature and optimize the indoor environment for the purpose of regulating the temperature, humidity, flow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/00C12Q1/02C12Q1/18
CPCC12Q1/18C12N1/20A61L2209/13A61L2209/16F24F8/50C12R2001/01C12N1/205C12R2001/44A01N63/00A61L2/22A61L9/14A61L2209/21C12Q1/689C12Q2600/158
Inventor 李太熙金智婉朴昭玧
Owner HYUNDAI MOTOR CO LTD
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