Fungus Glarea lozoyensis and application thereof for controlling microbial metabolite Pneumocandin Kangding B0

A technology of nemocontin and fungi, applied in new microorganisms and its application fields, can solve the problems of low yield, increased purification difficulty, unstable production capacity, etc., and achieve the effects of less fermentation by-products, excellent fermentation performance and stable production capacity

Active Publication Date: 2013-05-08
HANGZHOU HUADONG MEDICINE GRP PHARMA RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generic Neomercantin B 0 Bacteria are produced, the production capacity is unstable, the yield is low, the fermentation by-products are more, and the impurities are more, which leads to a more complicated post-extraction process, which greatly increases the difficulty of subsequent purification, and it is difficult to obtain a high-purity final product, so it is difficult Better industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: The culture, physiological and biochemical characteristics and carbon source utilization of the strain with the preservation number CCTCC NO: M2012475

[0027] 1. The morphological characteristics of the cultured strain with the preservation number CCTCC NO: M2012475

[0028] The strain with the preservation number CCTCC NO: M2012475 was inoculated in yeast malt extract agar medium (YME), potato dextrose agar medium (PDA), Sabouraud agar medium (SMA) and other medium for observing morphological characteristics , cultured at 23°C for 3 weeks, observed and described.

[0029] The colonies were cultured on YME agar medium at 23°C, with limited growth and a diameter of 11.7-15.4mm in 3 weeks; the colonies were nearly round, with irregular fan-shaped edges; the colonies were raised, and the center formed knots or collapsed into pits; the texture was velvety and rope-like ; Radioactive wrinkles are formed on the surface of the colony; the color of the mycelium is ...

Embodiment 2

[0044] Embodiment 2: Fermentation culture prepares pneumocidine B 0

[0045] The strain with the preservation number of CCTCC NO: M2012475 was used.

[0046] 1. Seed culture and storage

[0047] Solid medium: glucose 4g, yeast extract powder 0.5g, KH 2 PO 4 1g, 0.5g soybean peptone, 1.5g corn steep liquor powder, 2g agar, add water to 100mL, adjust pH to 7.0.

[0048] Solid culture method: the strain is inoculated on a solid medium slant and cultured at 26°C for 10-12 days.

[0049] After the solid culture is over, place the slant at 4-10°C and refrigerate for later use.

[0050] 2. Shake flask seed culture

[0051] Medium: glucose 4g, yeast extract powder 2.5g, corn steep liquor 1.5g, raw soybean powder 2g, KH 2 PO 4 1g, add water to 100mL, adjust pH to 7.0.

[0052] Filling volume: 100mL medium in a 500mL Erlenmeyer flask

[0053] Inoculum volume: 5% (V / V) 30% glycerol mycelium cryovial

[0054] Culture temperature: 26°C

[0055] Culture time: 3 days

[0056] ...

Embodiment 3

[0071] Embodiment 3: Fermentation culture prepares pneumocidine B 0

[0072] The strain with the preservation number CCTCC NO: M2012475 is used

[0073] Shake flask seed culture: each 100mL medium contains 2g of mannitol, 0.5g of yeast powder, 0.5g of corn steep liquor, 1g of soybean cake powder, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 1g, the rest is water, the pH is natural. Insert 5% (V / V) 30% glycerol mycelia freezing tube, 220rpm, 26°C for 3 days to obtain seed solution.

[0074] Seed tank culture: each 100mL medium contains 4g of mannitol, 1.5g of raw soybean powder, 0.5g of cottonseed powder, 1g of corn steep liquor powder, KH 2 PO 4 0.5g, the rest is water, the pH is natural. Seed inoculation amount 0.1% (V / V), 150rpm, culture at 26°C for 4 days.

[0075] Fermentation tank culture: each 100mL medium contains 8g of mannitol, 6g of glucose, 3g of cottonseed powder, 2g of yeast powder, KH 2 PO 4 0.3g, FeSO 4 ·7H 2 O 0.2g, L-serine 0.3g, L-arginine 0.2g, the rest is wa...

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Abstract

The invention discloses a fungus (Glarea lozoyensis) FIM 2006071. The strain is preserved in China Center for Type Culture Collection, the preservation number of the strain is CCTCC NO: M2012475, and the preservation date is 23rd, November, 2012. The invention further discloses a high-unit intermediate Pneumocandin Kangding B0 of caspofungin, which is obtained through controlling microbial metabolite. The fermentaition strain provided by the invention is good in performance, stable in production ability, high in fermentation unit and generates relatively less fermentation by-products, so that post-extraction difficulty is reduced, high-quality caspofungin is obtained favorably, and therefore, the strain is suitable for industrial mass production.

Description

technical field [0001] The present invention relates to a new type of microorganism and its application, in particular to a fungus Glarea lozoyensis and its ability to obtain nemocontin B by regulating the metabolism of microorganisms 0 Applications. Background technique [0002] Echinocandin, also known as echinocandin, is a new type of antifungal drug, belonging to acetyl hexacyclics, with low toxicity to the human body. It is a water-soluble lipopeptide, mainly used for the treatment of candida esophagitis or oral Pharyngitis, invasive aspergillosis, and other antifungal drugs that are ineffective or intolerant also have an effect on Pneumocystis carinii by inhibiting the synthesis of β(1,3)-D-glucose, an important component of the fungal cell wall. Antifungal effect. Caspofungin (Caspofungin) is the first approved echinocandin antifungal drug. For the treatment of patients with persistent fever and neutropenia, caspofungin, as an empirical treatment, has a high safety...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P21/04C12R1/645
Inventor 朱健陈晓霞王蓓许永锋吴娟严咪咪
Owner HANGZHOU HUADONG MEDICINE GRP PHARMA RES INST
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