The present invention relates to a method for operating a clarification setup (1) of a bioprocessing facility (2), the clarification setup (1) comprising a
fluidized bed centrifuge (3) and a pumping device (4), the
fluidized bed centrifuge (3) comprising at least one
centrifuge chamber (5) rotated about a geometric centrifuge axis (6), the bioprocessing facility (2) comprising an
electronic process controller (7) for controlling the
fluidized bed centrifuge (3) and the pumping device (4), the fluidized
bed centrifuge (3) comprising a particle loading cycle (8) and a pumping device (9), the fluidized
bed centrifuge (3) comprising at least one centrifuge chamber (5) rotated about a geometric centrifuge axis (6), the bioprocessing facility (2) comprising an
electronic process controller (7) for controlling the fluidized
bed centrifuge (3) and the pumping device (4), the fluidized bed centrifuge (3) comprising at least one centrifuge chamber (5) rotated about a geometric centrifuge axis (6), the bioprocessing facility (2) comprising at least one ... and / or a particle washing cycle (9) and a reverse operation for a particle
discharge cycle (10), and if a particle loading cycle (8) is provided, the
cell broth loaded into the centrifuge chamber (5) progresses to form a growing particle accumulation in the centrifuge chamber (5), and the clarification setup (1) includes a monitoring sensor device (11) with at least one optical sensor (12) for generating monitoring sensor data transmitted to an
electronic process control device (7). It is proposed that in a monitoring routine (13), image-related data (14) representative of an
optical image (15) of the centrifuge chamber contents (16) is generated by the monitoring sensor device (11) and a particle filling level (17) is calculated by the electronic
process control device (7) from the image-related data (14) based on a
computational model (18).