The invention discloses a measuring head and a non-contact tonometer calibrating device and method. The measuring head comprises a shell substrate, and an air inlet, a light guide column, a pressure sensor, a light-emitting device and a reflector which are arranged on the shell substrate; an air path is arranged in the air inlet and connected with the pressure sensor in a sealed mode, and the pressure sensor is used for detecting an air pressure signal of airflow sprayed by the non-contact tonometer; the light guide column is installed in the center of the air inlet and used for guiding light,and the curvature radius of the front end surface of the light guide column is matched with the curvature radius of the front surface of a real human cornea; the light-emitting device is installed onthe inner side of the light guide column and connected with the light guide column in the air inlet direction, light beams emitted by the light-emitting device are conducted out through the light guide column and received by the tonometer, and the light beam emitting time is related to the numerical value of an air pressure signal; and the reflector is arranged on the side edge of the air inlet and used for assisting in adjusting the angle of the measuring head. Good consistency of performance of the calibrating device is guaranteed, and long-term stability and accuracy of the calibrating device are improved.