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Fungus strain and uses thereof

A technology of strains and fungi, applied in fungi, applications, chemicals for biological control, etc., can solve problems such as lack of research and utilization, and achieve the effect of increasing biomass and promoting plant growth

Inactive Publication Date: 2009-07-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a lack of research and utilization of wild rice endophytic fungi in improving the growth of cultivated rice, increasing the biomass (yield), and improving its survivability in adversity.

Method used

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  • Fungus strain and uses thereof
  • Fungus strain and uses thereof
  • Fungus strain and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, isolation and cultivation of bacterial strain R5-6-1:

[0029] 2% malt extract agar medium (malt extract agar, MEA): malt extract powder 20g, agar 20g, distilled water 1000mL.

[0030] Potato dextrose agar medium (potato dextrose agar, PDA): potato 200g, glucose 20g, agar 20g, distilled water 1000mL.

[0031] In the laboratory, it can be isolated and cultured according to the following conditions to obtain the Phylinella oryzae described in the present invention. Collect wild rice (Oryza granulata) from Banna Nature Reserve in Yunnan, my country, rinse the collected wild rice with tap water, disinfect the rhizome of wild rice in 75% (v / v) alcohol solution for 30 seconds, and then disinfect it in 1% (v / v) v) Disinfect in sodium hypochlorite solution for 10 minutes, rinse with sterile water for 3 times. Cut the root into about 0.6cm long fragments with a sterile razor blade, and put them in 50μg·ml -1 Ampicillin and streptomycin on 2% malt juice agar (MEA)...

Embodiment 2

[0032] Embodiment 2, identification of bacterial strain R5-6-1:

[0033] Potato dextrose broth (PDB): 200 g of potatoes, 20 g of glucose, and 1000 mL of distilled water.

[0034] The relevant references on the morphological identification of Phialophora (Phialophora) fungi and the analysis of phylogenetic relationships using rDNA sequences are listed below. Biological Technology Guidebook.

[0035] Cole, G.T., and Kendrick, B. (1973). Taxonomic studies of Phialophora.Mycologia, Mycology, 5: 661-688, describe and summarize the morphological classification of Phiala fungi (Taxonomic studies of Phialophora.Mycologia, 65: 661-688.).

[0036] Deacon, J.W., and Scott, D.B. (1983). Phalaenopsis zea and its role in corn stalk rot, Communications of the British Mycological Society, 81: 247-262, reporting a new species of Phalaenopsis and its infection Corn stalk rot (Phialophora zeicola.sp.nov., and its role in the root rot-stalk rot complex of maize. Transactions of the British Myc...

Embodiment 3

[0056] Embodiment 3, bacterial strain R5-6-1 promotes the pot test of paddy rice growth and the microscopic observation that bacterial strain colonizes in root:

[0057] According to Newsham, K.K. (1999). The growth-promoting effect of dark septate endophyte-Philadinium graminearum on sagegrass, New Phytologists, 144:517-524, reported a dark septate endophyte Phthala graminearum Infect the root system of sagegrass and promote its growth (Phialophora graminicola, a dark septate ungus, is a beneficial associate of the grass Vulpia ciliata ssp. ambigua. New phytologist, 144:517-524.).

[0058] MS minimal medium: Potassium nitrate (KNO 3 ) 1900mg / L, ammonium nitrate (NH 4 NO 3 ) 1650mg / L, magnesium sulfate (MgSO 4 ·7H 2 O) 370mg / L, potassium dihydrogen phosphate (KH 2 PO 4 ) 170mg / L, calcium chloride (CaCl 2 2H 2 O) 440mg / L, manganese sulfate (MnSO 4 4H 2 O) 22.3mg / L, zinc sulfate (ZnSO 4 4H 2 O) 8.6mg / L, boric acid (H 3 BO 3 ) 6.2mg / L, potassium iodide (KI) 0.83mg / L...

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Abstract

The invention discloses a fungus strain, which is Phialophora oryzae R5-6-1 fungus strain with the preservation organization of China General Microbiological Culture Center of Microbial Culture Collection Management Committee, the preservation date of October 27th, 2008 and the preservation number of CGMCC 2737. The fungus strain can be used for improving the growth of vegetables especially paddy rice and increasing the live weight thereof.

Description

technical field [0001] The invention relates to an endophytic fungal strain of wild rice and its application in promoting plant growth and increasing biomass, and belongs to the field of microorganisms and microorganism applications. Background technique [0002] The interaction between plants and surrounding organisms is a common phenomenon, among which plant-microbe interaction is one of the important forms. There are two types of beneficial plant-microbial symbiosis: one has a nutritional function, such as nitrogen-fixing bacteria or mycorrhizal fungi; and the other has a defensive function, such as endophytes and epiphytes that inhibit pathogenic bacteria. Both classes of beneficial symbiotic microorganisms can enhance plant fitness. Agrochemicals (fertilizers and pesticides) and "intensive farming" cultivation methods play an important role in agricultural production. Under this cultivation method, the plant-microbe balance system in cultivated crops is broken, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
Inventor 章初龙林福呈袁志林
Owner ZHEJIANG UNIV
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