Korean pine introduction, cultivation and planting method

Abstract

The invention relates to the related technical field of introduction and cultivation of Korean pine, and discloses a Korean pine introduction, cultivation and planting method, which comprises the following steps: girdling a rootstock and applying special gel to guide nutrients and hormones in the rootstock into the gel, and then performing grafting or cuttage based on the gel. The girdling adopted in the scheme can enrich the nutrients of the rootstock to the maximum extent and prevent branches and leaves of the rootstock and the scion from grabbing the nutrients, meanwhile, the gel has good wrapping performance and can completely wrap the cut of the scion, compared with the approximate butt joint of the cortex of the traditional grafting rootstock and the cut of the scion, the interface is more tidy, and the scion can obtain more sufficient nutrients; the scion survival rate can be greatly improved; compared with a traditional cuttage method, according to the scheme, the scions are inserted into the gel, the scions can continuously obtain enough nutrients in the rooting process, and the cuttage survival rate is greatly increased.

Description

Technical Field

[0001] The invention relates to the technical field of introduction and cultivation of red pine, in particular to a method for introduction and cultivation of red pine. Background Art

[0002] Korean pine, also known as fruit pine and Korean pine, is a member of the Pinaceae family and an evergreen five-needle pine tree species. Natural Korean pine can be up to 40m tall and have a breast diameter of more than 2m. It is a common tree species in the Northeast forest area. The large-diameter Korean pine wood has excellent physical properties and is a good timber species for industry and household use. Korean pine is strong and abundant, and because Korean pine seeds are rich in oil, large in size, have a good taste and have certain therapeutic effects, there is a large social demand for it at home and abroad, and it has a high economic value. Therefore, in the national economic construction, Korean pine not only provides timber and protects the ecology, but has also become an important economic source for many foresters in the Northeast forest area. However, due to the wide range of uses of Korean pine wood, Korean pine has been over-logged. The natural Korean pine forest has been almost completely cut down, and the artificially created Korean pine forest requires a longer period of time. Asexual reproduction technology is an effective way to solve this problem.

[0003] However, due to the slow growth and development of red pine, the existing introduction and cultivation methods, including grafting and cuttings, have a low survival rate. The alignment of the interface during grafting is difficult to accurately control, the efficiency of nutrient delivery is limited, and it is easily disturbed by pests and diseases. Cutting propagation relies entirely on the nutrients stored in the branches themselves, resulting in insufficient nutrients and a low rooting rate. Summary of the invention

[0004] The purpose of the present invention is to provide a method for introducing and cultivating Korean pine to solve the problem of low survival rate of the current traditional introduction and cultivation method proposed in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a method for introducing and cultivating Korean pine, comprising the following steps: S1. Rootstock selection and treatment: First, select a healthy and pest-free Scots pine as the rootstock. Before grafting, prune the rootstock to remove weak, diseased and overcrowded branches. At the same time, gird the rootstock using a sharp knife to gird the cortex of the rootstock and remove the bark. S2. Preparation of gel matrix: The gel components include chitosan, sodium alginate, antibacterial agent, rooting promoter, cross-linking agent, humectant, and the balance is pH regulator and water. The above raw materials are fully mixed and configured into a gel; S3. Scion selection and processing: Select healthy, disease- and insect-free branches from the Korean pine mother tree as scion. Cut the bottom of the scion diagonally and remove excess leaves.

[0006] S4. Grafting preparation: First, spray a growth inhibitor on the prepared rootstock ring cut to inhibit the healing of the cortex at the rootstock cut. Then use a special grafting strip to wrap the rootstock cut. Then inject the prepared gel into the grafting strip and evacuate the air. Allow the gel to completely cover the cut, ensuring that the gel is in full contact with the cortex at the upper and lower ends of the cut. Then let it stand for 24 hours until the sap of the rootstock is completely fused with the gel, so that the cortex at the upper and lower ends of the ring cut can use the gel to exchange and transport water and nutrients.

[0007] S5. Indirect grafting: After the gel is prepared, insert the scion prepared in S3 into the gel so that the end of the scion is located between the upper and lower cortex of the rootstock ring cut, and seal it with plastic wrap.

[0008] S6. Rooting and transplanting: After the scion is inserted into the gel, it survives with the help of nutrients in the rootstock sap immersed in the gel. After a period of maintenance, the scion will take root in the gel and grow new branches and leaves. When the root system grows to 3-4cm, the scion can be removed and transplanted into the soil for subsequent transplanting.

[0009] Furthermore, the circumcision in S1 is deep enough to reach the cortex without damaging the xylem, and the width of the incision is between 1 cm and 2 cm.

[0010] Furthermore, the gel ratio is 10% chitosan, 8% sodium alginate, 0.5% antibacterial agent, 0.05% rooting promoter, 0.5% cross-linking agent, 3% moisturizing agent, and the balance is water. The pH regulator is based on the amount required to adjust the pH to a reasonable range.

[0011] Furthermore, the preparation method of the gel matrix comprises the following steps: S201. Preparation of chitosan solution: The weighed chitosan powder was slowly added to warm water, stirring while adding until a uniform suspension was formed. The amount of water was 5 times the mass of chitosan, and the water temperature was controlled at 50 ° C.

[0012] S202. Preparation of sodium alginate solution: Dissolve the weighed sodium alginate powder in 60°C warm water, stirring while adding, until a transparent viscous solution is formed in the warm water.

[0013] S203. Mixing and stirring: slowly pour the dissolved chitosan solution into the sodium alginate solution, and stir continuously with a stirrer to avoid caking. Then add precisely weighed antibacterial agent, rooting promoter, cross-linking agent and moisturizing agent in turn, and stir thoroughly after each addition.

[0014] S204. PH adjustment: Use a PH meter to measure the pH value of the mixed solution, and add PH adjuster as needed to adjust the PH to the same PH as the rootstock sap.

[0015] S205. Filtration and sterilization: Use a filter to filter the mixture to remove undissolved impurities, and then heat the filtered solution to 80°C in a water bath for 30 minutes for high-temperature sterilization to ensure sterility.

[0016] S206. Solidification and molding: Let the sterilized mixed liquid stand and cool to room temperature, allowing it to solidify into a gel.

[0017] Furthermore, in S204, the pH of the gel is finally adjusted to between 5.5 and 6.5.

[0018] Furthermore, in S3, the length of the scion is controlled between 9 and 12 centimeters and has at least 2 to 3 buds.

[0019] Furthermore, in S3, the grafting strip is made of flexible rubber material, and is provided with a semicircular glue injection groove inside, the glue injection groove is used to accommodate gel, and an insertion port is provided above the glue injection groove, and the scion is inserted into the gel through the insertion port.

[0020] Furthermore, the grafting strip surrounds the annular incision of the rootstock and is fixed to the rootstock by a double-sided adhesive strip.

[0021] Furthermore, in S5, when the scion is inserted into the gel, the lower end of the scion has no direct contact with the incision of the stock.

[0022] Furthermore, when transplanting in S6, the gel and the scion root system are taken out together for transplanting to avoid damaging the root system.

[0023] Compared with the prior art, the present invention has the following beneficial effects: The invention discloses a method for introducing and cultivating red pine. The method comprises the following steps: ring cutting a rootstock and applying a special gel to the rootstock, thereby guiding the nutrients and hormones in the rootstock into the gel, and then grafting or cutting is performed based on the gel. Compared with a traditional cross-cut grafting method, the ring cutting adopted in the present invention can enrich the rootstock nutrients to the greatest extent, and avoid the rootstock branches and leaves from competing with the scion for nutrients. Meanwhile, the gel also has good wrapping property and can completely wrap the incision of the scion. Compared with the rough docking of the traditional grafting rootstock cortex and the scion incision, the interface is more neat, the scion can obtain nutrients more fully, and the survival rate of the scion can be greatly improved. Compared with the traditional cutting method, the present invention inserts the scion into the gel in front, and the scion can also continuously obtain sufficient nutrients during the rooting process, so that the cutting survival rate is greatly improved. Moreover, compared with an artificially configured cutting matrix, the nutrients of the gel in the present invention come from the rootstock, and the gel also contains various biological hormones and local active substances. Compared with the artificial matrix, the gel is more complete and more conducive to cutting rooting. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1It is a schematic diagram of the cultivation method of the present invention; Figure 2 It is a schematic diagram of the grafting strip structure of the present invention.

[0025] Numbers in the figure: 1, grafting strip; 101, glue injection groove; 102, insertion port; 2, double-sided adhesive strip. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] In order to further understand the content of the present invention, the present invention is described in detail in conjunction with the accompanying drawings.

[0028] Embodiment 1: A method for introducing and cultivating Korean pine, comprising the following steps: 1. Rootstock selection and treatment: First, choose a healthy and pest-free Larix gmelinii as the rootstock to ensure that the root system is well developed and can stably absorb and transmit nutrients. Before grafting, prune the rootstock to remove weak, diseased and dense branches to reduce nutrient consumption and improve ventilation and light transmission of the grafted part. At the same time, gird the rootstock with a sharp knife to gird the cortex of the rootstock and remove the bark. The incision should be deep to the cortex but not to damage the xylem. The width of the incision should be between 1cm and 2cm, and the incision should be smooth to facilitate the adhesion of the gel and the absorption of nutrients.

[0029] The ring cutting operation completely cuts off the nutrient and water delivery channels of the Larix gmelinii rootstock, causing the nutrients and water to gather at the ring cutting point and gradually merge into the gel. Compared with the traditional cross-cutting method, the nutrient interception efficiency is higher, which is beneficial for the scion to compete with the original branches and leaves of the rootstock for nutrients, and can improve the survival rate and growth rate of the scion.

[0030] 2. Preparation of gel matrix: The gel components include chitosan 10%, sodium alginate 8%, antibacterial agent 0.5%, rooting promoter 0.05%, cross-linking agent 0.5%, moisturizing agent 3%, and the balance is pH regulator and water.

[0031] Chitosan, as the main matrix, has good adsorption, film-forming and permeability, which facilitates the enrichment and transfer of nutrients.

[0032] Sodium alginate has good film-forming properties and can form a stable three-dimensional network structure film after cross-linking with calcium ions. This film can not only effectively protect the grafted site from external mechanical damage and bacterial invasion, but also maintain a moist environment for the incision, which is conducive to cell regeneration and healing.

[0033] Rooting promoters can stimulate the root growth of the scion and accelerate the formation and development of the root system. This is crucial to improving the stress resistance and growth rate of the scion, and helps the scion adapt to the new growth environment as soon as possible and resume normal growth.

[0034] The cross-linking agent plays the role of connecting the matrix molecules in the gel and can form a stable gel network structure, which helps to improve the mechanical properties and water resistance of the gel and enables the gel to maintain a stable shape and performance during the operation process.

[0035] Humectants absorb and retain a certain amount of water, providing a continuously moist environment for the graft site, which is essential for maintaining cell activity and promoting the healing process.

[0036] The pH regulator is used to adjust the pH of the gel to make it as close as possible to the pH of the sap that delivers nutrients in the rootstock, helping to maintain the biological activity of the sap.

[0037] The preparation method of the gel matrix comprises the following steps: 1. Preparation of chitosan solution: Slowly add the weighed chitosan powder into warm water, stirring while adding, until a uniform suspension is formed. The amount of water is 5 times the mass of chitosan, and the water temperature is controlled at 50°C.

[0038] 2. Preparation of sodium alginate solution: Dissolve the weighed sodium alginate powder in 60°C warm water, stirring while adding, until a transparent viscous solution is formed in the warm water.

[0039] 3. Mixing and stirring: Slowly pour the dissolved chitosan solution into the sodium alginate solution, and stir continuously with a stirrer to avoid caking. Then add accurately weighed antibacterial agents, rooting promoters, cross-linking agents and moisturizers in turn, and stir thoroughly after each addition.

[0040] 4. PH adjustment: Use a PH meter to measure the pH value of the mixed solution, and add PH adjuster as needed to adjust the PH to between 5.5 and 6.5, which is consistent with the sap pH of red pine trees and facilitates the delivery of nutrients.

[0041] 5. Filtration and sterilization: Use a filter to filter the mixture to remove undissolved impurities, and then heat the filtered solution to 80°C in a water bath for 30 minutes for high temperature sterilization to ensure sterility.

[0042] 6. Solidification and molding: Let the sterilized mixed liquid stand and cool to room temperature, allowing it to solidify into a gel.

[0043] 3. Scion selection and processing: Select healthy, disease- and insect-free branches from the Korean pine mother tree as scion. The scion length should be between 9 and 12 cm, with at least 2 to 3 buds. Cut the bottom of the scion obliquely and remove excess leaves to reduce water evaporation and nutrient consumption.

[0044] 4. Grafting preparation: First, spray the growth inhibitor on the prepared rootstock ring cut to inhibit the healing of the cortex at the rootstock cut, so that the rootstock cut is exposed to the gel for a longer time, increase the effective retention time of the gel, and provide a longer guarantee time for the rooting of the scion. Then use a special grafting strip 1 to wrap the rootstock cut, and then inject the prepared gel into the grafting strip 1, and evacuate the air, so that the gel completely covers the cut, ensuring that the gel is in full contact with the cortex at the upper and lower ends of the cut, and then let it stand for 24 hours, until the sap of the rootstock is completely fused with the gel, so that the cortex at the upper and lower ends of the ring cut can use the gel to exchange and transport water and nutrients.

[0045] 5. Indirect grafting: After the gel is prepared, insert the scion prepared in S3 into the gel so that the end of the scion is located between the upper and lower cortex of the rootstock ring cut, the lower end of the scion does not touch the rootstock cut, and the insertion port is sealed with plastic wrap.

[0046] 6. Rooting and transplanting: After the scion is inserted into the gel, it survives with the help of nutrients in the rootstock sap immersed in the gel. After a period of maintenance, the scion will take root in the gel and grow new branches and leaves. When the root system grows to 3-4cm, the scion can be removed and transplanted into the soil for subsequent transplanting. When transplanting, take out the gel and the scion roots together to avoid damaging the roots.

[0047] Example 2: A method for introducing and cultivating Korean pine, which can be grafted to achieve economic benefits quickly, specifically includes the following steps: 1. Rootstock selection and treatment: First, select a healthy and pest-free Scots pine as the rootstock to ensure that the rootstock has a well-developed root system and can stably absorb and transmit nutrients. Before grafting, prune the rootstock to remove weak branches, diseased branches and overcrowded branches to reduce nutrient consumption and improve ventilation and light transmission of the grafted part. At the same time, gird the rootstock with a sharp knife to gird the cortex of the rootstock and remove the bark. The incision depth should be to the cortex but not to damage the wood. The incision width should be between 0.5 and 1 cm, and the incision should be smooth and flat to facilitate the adhesion of gel and the absorption of nutrients. 2. Preparation of gel matrix: The gel components include chitosan 10%, sodium alginate 8%, antibacterial agent 0.5%, cross-linking agent 0.5%, moisturizing agent 3%, and the balance is pH regulator and water.

[0048] Compared with Example 1, the rooting promoter is eliminated, so that the scion is prevented from taking root, and the scion can grow and merge with the incision.

[0049] The preparation method of the gel matrix comprises the following steps: 1. Preparation of chitosan solution: Slowly add the weighed chitosan powder into warm water, stirring while adding, until a uniform suspension is formed. The amount of water is 5 times the mass of chitosan, and the water temperature is controlled at 50°C.

[0050] 2. Preparation of sodium alginate solution: Dissolve the weighed sodium alginate powder in 60°C warm water, stirring while adding, until a transparent viscous solution is formed in the warm water.

[0051] 3. Mixing and stirring: Slowly pour the dissolved chitosan solution into the sodium alginate solution, and stir continuously with a stirrer to avoid caking. Then add accurately weighed antibacterial agents, rooting promoters, cross-linking agents and moisturizers in turn, and stir thoroughly after each addition.

[0052] 4. PH adjustment: Use a PH meter to measure the pH value of the mixed solution, and add PH adjuster as needed to adjust the PH to between 5.5 and 6.5, which is consistent with the sap pH of red pine trees and facilitates the delivery of nutrients.

[0053] 5. Filtration and sterilization: Use a filter to filter the mixture to remove undissolved impurities, and then heat the filtered solution to 80°C in a water bath for 30 minutes for high temperature sterilization to ensure sterility.

[0054] 6. Solidification and molding: Let the sterilized mixed liquid stand and cool to room temperature, allowing it to solidify into a gel.

[0055] 3. Scion selection and processing: Select healthy, disease- and insect-free branches from the Korean pine mother tree as scion. The scion length should be between 9 and 12 cm, with at least 2 to 3 buds. Cut the bottom of the scion obliquely and remove excess leaves to reduce water evaporation and nutrient consumption.

[0056] 4. Grafting preparation: First, wrap the rootstock incision with a special grafting strip 1, then inject the prepared gel into the grafting strip 1, and exhaust the air, so that the gel completely covers the incision, ensuring that the gel is in full contact with the cortex at the upper and lower ends of the incision. Then let it stand for 24 hours until the sap of the rootstock and the gel are completely fused, so that the cortex at the upper and lower ends of the girdling incision can use the gel to exchange and transport water and nutrients.

[0057] 5. Indirect grafting: After the gel is prepared, insert the scion prepared in S3 into the gel so that the end of the scion is located between the upper and lower cortical layers of the rootstock circumcision, and the end of the scion is in close contact with the upper or lower cortical layer of the rootstock circumcision, and the insertion port is sealed with plastic wrap. After the scion is inserted into the gel, it survives with the help of nutrients in the rootstock sap immersed in the gel. After a period of maintenance, the upper and lower cortical layers of the rootstock circumcision grow and fuse together, and the scion is fused together, realizing dense grafting of the scion.

[0058] The grafting strip 1 used in the above-mentioned embodiment 1 and embodiment 2 is made of flexible rubber material, and a semicircular glue injection groove 101 is provided inside, and the glue injection groove 101 is used to accommodate gel, and an insertion port 102 is provided above the glue injection groove 101, and the insertion port 102 is also the injection port of the gel, and the scion is inserted into the gel through the insertion port 102. The grafting strip 1 surrounds the annular incision of the stock and is fixed on the stock by the double-sided adhesive strip 2 to ensure sealing. The grafting strip 1 is specifically provided for fixing the gel, which reduces the difficulty of gel wrapping and reduces the pollution of the gel by the external environment.

[0059] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for introducing and cultivating Korean pine, characterized in that: The following steps are involved: S1. Rootstock selection and treatment: First, select a healthy and pest-free Scots pine as the rootstock. Before grafting, prune the rootstock to remove weak, diseased and overcrowded branches. At the same time, gird the rootstock using a sharp knife to gird the cortex of the rootstock and remove the bark. S2. Preparation of gel matrix: The gel components include chitosan, sodium alginate, antibacterial agent, rooting promoter, cross-linking agent, humectant, and the balance is pH regulator and water. The above raw materials are fully mixed and configured into a gel; S3. Scion selection and processing: Select healthy, full-bud, disease-free and insect-free branches from Korean pine mother trees as scions, cut the bottom of the scion diagonally and remove excess leaves; S4. Grafting preparation: First, spray a growth inhibitor on the prepared rootstock ring cut to inhibit the healing of the cortex at the rootstock ring cut, then use a special grafting strip (1) to wrap the rootstock ring cut, and then inject the prepared gel into the grafting strip (1), and evacuate the air, so that the gel completely covers the cut, ensuring that the gel is in full contact with the cortex at the upper and lower ends of the cut, and then let it stand for 24 hours until the sap of the rootstock and the gel are completely fused, so that the cortex at the upper and lower ends of the ring cut can exchange and transport water and nutrients with the help of the gel; S5. Indirect grafting: After the gel is prepared, insert the scion prepared in S3 into the gel so that the end of the scion is located between the upper and lower cortex of the rootstock ring cut, and seal it with plastic wrap; S6. Rooting and transplanting: After the scion is inserted into the gel, it survives with the help of nutrients in the rootstock sap immersed in the gel. After a period of maintenance, the scion will take root in the gel and grow new branches and leaves. When the root system grows to 3-4cm, the scion can be removed and transplanted into the soil for subsequent transplanting.

2. The method for introducing, cultivating and planting Korean pine according to claim 1, characterized in that: The depth of the circumcision in S1 reaches the cortex but does not damage the xylem, and the width of the incision is between 1 cm and 2 cm.

3. The method for introducing, cultivating and planting Korean pine according to claim 1, characterized in that: The gel ratio is 10% chitosan, 8% sodium alginate, 0.5% antibacterial agent, 0.05% rooting promoter, 0.5% cross-linking agent, 3% moisturizing agent, and the balance is water. The pH regulator is based on the amount required to adjust the pH to a reasonable range.

4. The method for introducing, cultivating and planting Korean pine according to claim 1, characterized in that: The preparation method of the gel matrix comprises the following steps: S201 chitosan solution preparation: The weighed chitosan powder was slowly added to warm water, stirring while adding until a uniform suspension was formed, the amount of water was 5 times the mass of chitosan, and the water temperature was controlled at 50 ° C; S202. Preparation of sodium alginate solution: The weighed sodium alginate powder was dissolved in warm water at 60 ° C, and the mixture was stirred until a transparent viscous solution was formed in warm water; S203. Mixing and stirring: The dissolved chitosan solution is slowly poured into the sodium alginate solution, and stirred continuously with a stirrer to avoid agglomeration, and then the precisely weighed antibacterial agent, rooting promoter, cross-linking agent and moisturizing agent are added successively, and each addition is thoroughly stirred; S204. PH adjustment: Use a PH meter to measure the pH value of the mixed solution, and add a PH regulator as needed to adjust the pH to the same pH as the rootstock sap; S205. Filtration and sterilization: Filter the mixture using a filter to remove undissolved impurities, and then heat the filtered solution to 80 ° C in a water bath for 30 minutes for high temperature sterilization to ensure sterility; S206. Solidification and molding: Let the sterilized mixed liquid stand and cool to room temperature, allowing it to solidify into a gel.

5. The method for introducing, cultivating and planting Korean pine according to claim 4, characterized in that: In S204, the pH of the gel is finally adjusted to between 5.5 and 6.

5.

6. The method for introducing, cultivating and planting Korean pine according to claim 1, characterized in that: In S3, the length of the scion is controlled between 9 and 12 centimeters and has at least 2 to 3 buds.

7. The method for introducing, cultivating and planting Korean pine according to claim 1, characterized in that: In S3, the grafting strip (1) is made of a flexible rubber material and is provided with a semicircular glue injection groove (101) inside. The glue injection groove (101) is used to accommodate gel. An insertion opening (102) is provided above the glue injection groove (101), and the scion is inserted into the gel through the insertion opening (102).

8. The method for introducing, cultivating and planting Korean pine according to claim 7, characterized in that: The grafting strip (1) surrounds the annular incision of the rootstock and is fixed on the rootstock via a double-sided adhesive strip (2).

9. The method for introducing, cultivating and planting Korean pine according to claim 1, characterized in that: In S5, when the scion is inserted into the gel, the lower end of the scion has no direct contact with the incision of the stock.

10. The method for introducing, cultivating and planting Korean pine according to claim 1, characterized in that: When transplanting in S6, the gel and the scion root system are taken out together for transplanting to avoid damaging the root system.

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