Mutation breeding method for cunninghamia lanceolata
A technology of Chinese fir and seeds, applied in the field of mutation breeding of Chinese fir
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Embodiment 1
[0100] Example 1 Colchicine mutagenesis treatment
[0101] 1. Seed source
[0102] In November 2011, the mature cones of Chinese fir were collected in the seed garden of the state-owned forest farm in Jiangle County, Fujian Province, and the dried seeds were collected and then refrigerated at 4°C for 3 months. As the mutagenic seed material in this experiment.
[0103] 2. Gibberellin soaking treatment
[0104] This study started in February 2012, and in February, the low-temperature treated seeds were soaked in gibberellin.
[0105] 2-1 Under the condition of constant room temperature (25±5°C, 25°C is selected in the embodiment of the present invention), first soak the dry seeds of Chinese fir in a gibberellin solution with a concentration of 25mg / L, after soaking for 24h, wash with distilled water Seed 5 times, remove gibberellin residual liquid on the surface of Chinese fir seed, obtain gibberellin seed soaking treatment seed.
[0106] Soak the seeds to improve seed germ...
Embodiment 2
[0124] Example 2 Ethyl methanesulfonate (EMS) mutagenesis treatment of Chinese fir seeds
[0125] 1. Seed source
[0126] Same as "seed source" in embodiment 1.
[0127] 2. Gibberellin soaking treatment
[0128] Same as "Gibberellin Soaking Treatment" in Example 1.
[0129] 3. Soaking in distilled water
[0130] Same as "distilled water immersion treatment" in embodiment 1.
[0131] 4. Mutagenesis treatment
[0132] Under the conditions of room temperature (25±5° C.) and completely protected from light (that is, darkness), the Chinese fir seeds soaked in distilled water were subjected to EMS mutagenesis treatment.
[0133] The test set 4 concentration gradients (0.3%, 0.6%, 0.9%, 1.2% w / v), 3 treatment times (8h, 12h, 16h), wherein the preparation method of EMS solution is to dissolve EMS in pH=7.0, 0.1mol / L phosphate buffer. That is to say, 0.3g, 0.6g, 0.9g, and 1.2g EMS are respectively dissolved in 100mL of pH=7.0, and 0.1mol / L phosphate buffer is made into the EMS m...
Embodiment 3
[0143] Example 3 Sodium azide (NaN 3 ) mutagenesis treatment of Chinese fir seeds
[0144] 1. Source of seeds:
[0145] Same as "seed source" in embodiment 1.
[0146] 2. Gibberellin soaking treatment
[0147] Same as "Gibberellin Soaking Treatment" in Example 1.
[0148] 3. Soaking in distilled water
[0149] Same as "distilled water immersion treatment" in embodiment 1.
[0150] 4. Mutagenesis treatment
[0151] Under the conditions of room temperature (25±5°C) and completely protected from light (that is, darkness), the Chinese fir seeds soaked in distilled water were subjected to sodium azide mutagenesis treatment.
[0152] Five concentration gradients (2, 4, 6, 8, 10mmol / L, prepared with pH=3.0, 0.1mol / L phosphate buffer) were set in the experiment, and the gibberellin soaked seeds were respectively soaked in sodium azide solution In 25°C, shake (120 rpm) for 4h, 8h, and 12h, and then wash the mutagenized seeds under running water for 2h to remove the residual sodi...
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