Mutation breeding method for cunninghamia lanceolata

A technology of Chinese fir and seeds, applied in the field of mutation breeding of Chinese fir

Inactive Publication Date: 2015-04-29
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Up to now, there is only one report on the creation and screening of new Chinese fir germplasm using the

Method used

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  • Mutation breeding method for cunninghamia lanceolata
  • Mutation breeding method for cunninghamia lanceolata
  • Mutation breeding method for cunninghamia lanceolata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Example 1 Colchicine mutagenesis treatment

[0101] 1. Seed source

[0102] In November 2011, the mature cones of Chinese fir were collected in the seed garden of the state-owned forest farm in Jiangle County, Fujian Province, and the dried seeds were collected and then refrigerated at 4°C for 3 months. As the mutagenic seed material in this experiment.

[0103] 2. Gibberellin soaking treatment

[0104] This study started in February 2012, and in February, the low-temperature treated seeds were soaked in gibberellin.

[0105] 2-1 Under the condition of constant room temperature (25±5°C, 25°C is selected in the embodiment of the present invention), first soak the dry seeds of Chinese fir in a gibberellin solution with a concentration of 25mg / L, after soaking for 24h, wash with distilled water Seed 5 times, remove gibberellin residual liquid on the surface of Chinese fir seed, obtain gibberellin seed soaking treatment seed.

[0106] Soak the seeds to improve seed germ...

Embodiment 2

[0124] Example 2 Ethyl methanesulfonate (EMS) mutagenesis treatment of Chinese fir seeds

[0125] 1. Seed source

[0126] Same as "seed source" in embodiment 1.

[0127] 2. Gibberellin soaking treatment

[0128] Same as "Gibberellin Soaking Treatment" in Example 1.

[0129] 3. Soaking in distilled water

[0130] Same as "distilled water immersion treatment" in embodiment 1.

[0131] 4. Mutagenesis treatment

[0132] Under the conditions of room temperature (25±5° C.) and completely protected from light (that is, darkness), the Chinese fir seeds soaked in distilled water were subjected to EMS mutagenesis treatment.

[0133] The test set 4 concentration gradients (0.3%, 0.6%, 0.9%, 1.2% w / v), 3 treatment times (8h, 12h, 16h), wherein the preparation method of EMS solution is to dissolve EMS in pH=7.0, 0.1mol / L phosphate buffer. That is to say, 0.3g, 0.6g, 0.9g, and 1.2g EMS are respectively dissolved in 100mL of pH=7.0, and 0.1mol / L phosphate buffer is made into the EMS m...

Embodiment 3

[0143] Example 3 Sodium azide (NaN 3 ) mutagenesis treatment of Chinese fir seeds

[0144] 1. Source of seeds:

[0145] Same as "seed source" in embodiment 1.

[0146] 2. Gibberellin soaking treatment

[0147] Same as "Gibberellin Soaking Treatment" in Example 1.

[0148] 3. Soaking in distilled water

[0149] Same as "distilled water immersion treatment" in embodiment 1.

[0150] 4. Mutagenesis treatment

[0151] Under the conditions of room temperature (25±5°C) and completely protected from light (that is, darkness), the Chinese fir seeds soaked in distilled water were subjected to sodium azide mutagenesis treatment.

[0152] Five concentration gradients (2, 4, 6, 8, 10mmol / L, prepared with pH=3.0, 0.1mol / L phosphate buffer) were set in the experiment, and the gibberellin soaked seeds were respectively soaked in sodium azide solution In 25°C, shake (120 rpm) for 4h, 8h, and 12h, and then wash the mutagenized seeds under running water for 2h to remove the residual sodi...

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Abstract

The invention discloses a mutation breeding method for cunninghamia lanceolata. The method comprises the following steps: especially adopting ripen seeds of a cunninghamia lanceolata seed garden; carrying out low-temperature overwintering treatment, carrying out constant-temperature seed soaking stimulation on a gibberellin solution with specific concentration, immediately putting the gibberellin solution subjected to constant-temperature seed soaking stimulation into clear water, carrying out variable-temperature soaking for 3 days, and carrying out sprout promoting treatment; respectively carrying out colchicine mutation treatment, ethylmethane sulfonate (EMS) mutation treatment and sodium azide (NaN3) mutation treatment; and finally seeding and germinating the cunninghamia lanceolata seeds which are subjected to mutation treatment, so as to obtain a large group of cunninghamia lanceolata mutation seedlings. The cunninghamia lanceolata mutation seedlings obtained by the mutation method have the characteristics of obvious phenotypic characteristic variation, large population growth characteristic variation range and the like; a great number of mutated cunninghamia lanceolata materials can be formed within a short period of time by the method disclosed by the invention; and a foundation is laid for breeding of a new variety of cunninghamia lanceolata.

Description

technical field [0001] The invention relates to a plant mutation breeding method, in particular to a mutation breeding method for increasing the mutation rate and creating new materials, and belongs to the technical field of Chinese fir mutation breeding. Background technique [0002] Chinese fir (Cunninghamia Lanceolata Lamb.Hook) is a unique afforestation timber species in southern my country. It has a long history of cultivation and a wide distribution area. It has always been the main research object of forest genetic improvement in my country. Since the beginning of provenance testing in the 1950s, Chinese fir high-generation seed orchards have produced high yields. In this breeding process, economic indicators such as plant height, diameter at breast height, and wood properties are often retained as important target traits, while other stress resistance traits such as drought resistance, insect resistance, and barren resistance are easily ignored, resulting in more gen...

Claims

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Application Information

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IPC IPC(8): A01H1/06
Inventor 李云胡瑞阳孙宇涵吴博方禄明郑仁华赵克奇段红静郑会全马超
Owner BEIJING FORESTRY UNIVERSITY
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