Halomonas huanghegensis BJGMM-B45 and application thereof

A technology of BJGMM-B45 and Halomonas, applied in the field of microorganisms, can solve problems that restrict agricultural development and affect sustainable development

Inactive Publication Date: 2014-05-21
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Yellow River Delta is located in the north of Shandong Province. Its unused land area is about 540,000 hectares, of which the saline-alkali land area accounts for abo

Method used

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  • Halomonas huanghegensis BJGMM-B45 and application thereof
  • Halomonas huanghegensis BJGMM-B45 and application thereof
  • Halomonas huanghegensis BJGMM-B45 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The isolation culture method of embodiment 1 Halomonas yellow river BJGMM-B45

[0037] Soil samples were collected from undisturbed saline-alkali areas in the Yellow River Delta Nature Reserve. Weigh about 1g of soil sample, add it to 9ml of 0.9% normal saline, and shake the bacteria at 30°C for 24h. Then perform gradient dilution, spread on beef extract peptone medium (0.5g beef extract, 1.0g peptone, 0.5g NaCl, 1.5-2.0g agar, add water to 100ml, pH7.2), separate and cultivate soil sample bacteria. On beef extract peptone medium with a NaCl concentration of 150g / l, flip the plate and place it in an incubator at 30°C for 2 days, then isolate and cultivate Halomonas huanghegensis (Halomonas huanghegensis) BJGMM-B45 of the present invention, its preservation number It is CGMCC NO.7022. The colonies are white, round, with neat edges, raised and smooth surface, and 0.5-1cm in diameter.

Embodiment 2

[0038] The salt tolerance test of embodiment 2 Halomonas yellow river BJGMM-B45

[0039] 1.1 Test method

[0040] Using LB liquid medium with different NaCl concentrations (0, 10, 20, 50, 80, 100, 110, 130, 150, 180 and 200g / l) to identify the optimal salinity tolerance of Halomonas yellow Bacillus (E.coli K12, CGMCC1.365) was used as a control. Prepare the seed solution, insert it into LB liquid medium with 5% inoculum amount, cultivate it at 30°C, 200r / min for 12 hours, measure the OD value at 600nm, and compare their growth conditions.

[0041] 1.2 Results

[0042] The OD value at 600nm of the strain BJGMM-B45 was 6 times higher than that of the control strain in the salt environment of 80, 100, 110, and 130g / L NaCl respectively (Table 2 and figure 2 ).

[0043] Table 2. The growth of Halomonas yellowheus BJGMM-B45 under different salt concentrations

[0044]

[0045] Note: ND means not determined.

Embodiment 3

[0046] Example 3 Antibiotic tolerance test of Halomonas yellowheus BJGMM-B45

[0047] 1.1 Test method

[0048] The LB liquid medium of different antibiotics was used to test the antibiotic resistance of Halomonas yellowheus: the working concentrations of chloramphenicol, streptomycin, ampicillin, rifampicin and tetracycline hydrochloride were: 25μg / ml, 50μg / ml , 50 μg / ml, 150 μg / ml and 50 μg / ml.

[0049] 1.2 Results

[0050] Halomonas yellow river BJGMM-B45 has antibiotic resistance, shows good resistance to chloramphenicol (25 μ g / ml) and streptomycin (50 μ g / ml) ( image 3 ).

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Abstract

The invention provides a bacteria halomonas huanghegensis BJGMM-B45, which has excellent salt tolerance. The preservation number of the halomonas huanghegensis BJGMM-B45 is CGMCC (China General Microbiological Culture Collection Center) NO.7022. The bacteria not only can be directly used as functional bacteria for improvement of saline-alkali soil, but also can be used as a gene donor of a novel transgenic organism; the organism can obtain excellent stress resistance if an excellent salt tolerance gene is transferred into the organism; in addition, the bacteria also can be used as a novel gene engineering bacteria, and can obtain more excellent properties by accepting other foreign excellent genes; furthermore, more mutant strains with excellent properties can be obtained through improvement of properties by traditional mutation breeding manner.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a newly isolated salt-tolerant bacterium-Halomonas yellow river BJGMM-B45 and its application. Background technique [0002] There are about one billion hectares of saline-alkali land on land in the world, of which the area of ​​saline-alkali land in my country is about 34.6 million hectares. The Yellow River Delta is located in the north of Shandong Province. Its unused land area is about 540,000 hectares, of which the saline-alkali land area accounts for about one-third, or as high as 180,000 hectares. This has seriously affected the sustainable development of the regional economy. And restrict the development of agriculture. There are many reasons for the formation of saline-alkali land, mainly due to the low altitude in the Yellow River Delta region, shallow groundwater burial, unstable soil structure, high degree of mineralization, and strong natural evaporation, r...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14C12R1/01C09K101/00
Inventor 苗朝华金芜军宛煜嵩贾芳芳
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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