Strain for producing cellulase and application of strain

A technology of cellulase and bacterial strains, applied in the field of microbiology, can solve the problems of less reports on Penicillium, and achieve the effects of simple operation, reduced enzyme cost, and low requirements for equipment

Inactive Publication Date: 2014-02-05
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Trichoderma, Aspergillus, etc. have been studied more, such as Trichoderma reesei and Aspergill

Method used

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  • Strain for producing cellulase and application of strain
  • Strain for producing cellulase and application of strain
  • Strain for producing cellulase and application of strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the cultivation of departure bacterial strain

[0028] Penicillium juniperus strain 9-3 was continuously activated twice on a PDA slant, cultured in an incubator at 30°C for 3-4 days, and then stored at 4°C for use.

[0029] Penicillium piceum 9-3. Deposit unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, deposit address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, deposit date: October 9, 2011, deposit number: CGMCC5314.

Embodiment 2

[0030] Embodiment 2: diethyl sulfate mutagenesis screening bacterial strain H16 (such as figure 1 shown)

[0031] 1) Diethyl sulfate mutagenesis

[0032] Wash the spores cultivated on the PDA slant with pH 7 phosphate buffer, break up the sterile glass beads, filter with two layers of sterile lens paper to obtain the spore suspension, count on a hemocytometer, and adjust the spore concentration to 10 6 per mL, take 4 mL of spore suspension, add it to 16 mL of phosphate buffer solution with pH 7, then add 0.2 mL of diethyl sulfate, start the reaction at 30°C and 150 r / min for 20 minutes, then take the reaction every 5 minutes Solution 0.1mL was added to 0.9mL of 25% sodium thiosulfate and mixed thoroughly to terminate the mutagenesis reaction, and then the mixed solution was diluted with three gradients to make the final concentration of bacteria in the mixed solution be 10 5 pcs / mL, 10 4 pcs / mL, 10 3 0.1mL of the dilutions of three concentrations were applied to a PDA plat...

Embodiment 3

[0040]Embodiment 3: strain genetic stability

[0041] The genetic stability of the highly active bacterial strain H16 obtained in Example 2 was investigated. The strain was continuously transferred for 6 generations, and each generation was inserted into the fermentation medium described in Example 2 for fermentation. As shown in Table 2 below, strain H16 has high enzyme activity and good stability.

[0042]

[0043] Table 2

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Abstract

The invention discloses a strain for producing cellulase. The strain has a collection number of CGMCC No.8339 in the China General Microbiological Culture Collection Center, and the classification name of the strain is Penillium piceum H16. The Penillium piceum is obtained through induced mutation treatment by taking a strain Penillium piceum 9-3 screened from straws as an original strain. The Penillium piceum 9-3 serves as the original strain for mutation breeding, the Penillium piceum H16 for producing high-activity cellulase is obtained through breeding, the enzyme activity of filter paperof cellulase crude enzyme liquid obtained by fermenting the Penillium piceum H16 reaches 7IU/mL, and the enzyme activity of beta-glucosidase reaches 50IU/mL. Different cellulase systems of the Penillium piceum H16 and Trichoderma reesei RUT-C30 are researched according to different proportions, the enzyme activity and the hydrolysis rate of microcrystalline celluloses are greatly improved at an optimal proportion, and the enzyme cost is reduced.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a strain producing cellulase and its application. Background technique [0002] Since the beginning of the 21st century, human beings are facing more and more severe challenges in energy, resources, and the environment. Cellulose is the largest renewable organic resource on the earth. Using these cheap and renewable plant cellulose raw materials to produce bio-based products and biomass energy is very beneficial to the sustainable development of human beings. Cellulose is the main component of plant cell walls, and is the most widely distributed and most abundant polysaccharide in nature, accounting for more than 50% of the carbon content in the plant kingdom. In my country, hundreds of millions of tons of crop stalks are burned every year, which not only pollutes the environment but also wastes resources. At present, the methods of cellulose saccharification mainly include acid hyd...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/01C12N9/42C12R1/80
CPCC12N9/2437C12N15/01C12N1/145C12R2001/80
Inventor 陈树林张粲宗志友贾文娣赫荣琳武改红李晨张东远
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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