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Fungus strain with high-efficiency expression of huperzine A and application thereof

A Huperzine A, high-efficiency expression technology, applied in the field of microorganisms, can solve the problems of high cost, lack of plant resources, difficult to obtain pure optically active compounds, etc., and achieves the goal of reducing medical expenses, protecting plant resources, and solving drug needs. Effect

Active Publication Date: 2016-06-15
陕西仁达康健医药生物科技有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to provide a fungal strain that efficiently expresses huperzine A, which solves the bottleneck of plant resource shortage in obtaining huperzine A from plant extraction; and the chemical synthesis steps are cumbersome and expensive, and it is difficult to obtain pure optically active compounds The problem

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  • Fungus strain with high-efficiency expression of huperzine A and application thereof
  • Fungus strain with high-efficiency expression of huperzine A and application thereof
  • Fungus strain with high-efficiency expression of huperzine A and application thereof

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Embodiment Construction

[0021] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0022] The present invention is a fungal strain for highly expressing huperzine A, which is isolated from wild Huperziaserrata (Huperziaserrata Huperzaceae, Huperzia genus) endophytic fungus and belongs to the genus Mucorracemosus The NSH-D strain has genetic stability after 15 generations in the laboratory, and its amino sequence is shown in SEQ ID NO: 1.

[0023] The strain has been preserved in China Ordinary Microorganism Culture Collection Center, a preservation unit designated by the State Intellectual Property Office.

[0024] Biological material deposit information:

[0025] Name: Mucorracemosus NSH-D strain

[0026] Deposit number: CGMCCNo.12077

[0027] Deposit unit: General Microbiology Center of China Committee for Microorganism Culture Collection (CGMCC)

[0028] Preservation time: January 15, 2016

[0029] Address: No. 3, Y...

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Abstract

The invention discloses a fungus strain with high-efficiency expression of huperzine A. The strain is separated from endophytic fungi of wild huperzia serrata (huperziaceae, huperzia), belongs to the NSH-D strain of mucor racemosus and has genetic stability till the passage to 15th generation in a laboratory; and the amino acid sequence is shown by SEQ ID NO:1. The fungus strain with high-efficiency expression of huperzine A, disclosed by the invention, serves as a biological strain for treating the Alzheimer's disease, the good huperzine A generating high-efficiency expression gene is transferred into other type strains, and more good characters are obtained through gene modification; and moreover, further optimization of the strain can be realized through mutation breeding. The study on the fungus strain is of great significance to the biosynthesis and industrial production of huperzine A, protection of plant resources, meeting of clinical pharmaceutical needs and reduction of the medical expense for treating the Alzheimer's disease.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a fungal strain highly expressing huperzine A, and also relates to the application of the fungal strain. Background technique [0002] Currently, the class of acetylcholinesterase inhibitors dominates the Alzheimer's disease drug market. Huperzine A is a new type of lycopodium monomeric alkaloid isolated from the folk herbal medicine Huperzia serrata. It is a potent and reversible cholinesterase inhibitor with multi-target effects. The inhibitory effect is 3 times that of physostigmine and 30 times that of galantamine, and the peripheral adverse reactions are low. It is one of the clinically preferred drugs for the treatment of Alzheimer's disease. [0003] The development of drugs derived from natural plants has a long history and is full of vitality. Plant extraction to obtain huperzine A is the easiest way, but plant extraction to obtain huperzine A has faced the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/06A61P25/28C12R1/785
CPCA61K36/06C12N1/14C12R2001/785C12N1/145
Inventor 韩文霞李伟泽韩忠文贾敏张寒孙静武永红周永强
Owner 陕西仁达康健医药生物科技有限公司
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