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Application of bacillus licheniformis to promotion of rooting or growth of semen trigonellae tissue culture seedlings

A technology for Bacillus licheniformis and tissue culture seedlings, which is applied in application, plant regeneration, gardening and other directions, can solve the problems of long rooting cycle, low rooting rate, low reproduction coefficient, etc., and achieves improved rooting quality, survival rate and rooting rate increase effect

Inactive Publication Date: 2014-06-04
贡格尔(北京)农牧科技有限公司
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  • Description
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  • Application Information

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Problems solved by technology

However, due to its limited source of materials and low reproduction coefficient, large-scale production cannot be realized, and tissue culture is an important way to solve this problem
However, conventional methods are slow to proliferate and popularize, blueberry test-tube seedlings are difficult to root, the rooting rate is low, and the rooting cycle is long, which restricts the process of industrialized seedling cultivation of fine varieties.

Method used

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  • Application of bacillus licheniformis to promotion of rooting or growth of semen trigonellae tissue culture seedlings
  • Application of bacillus licheniformis to promotion of rooting or growth of semen trigonellae tissue culture seedlings
  • Application of bacillus licheniformis to promotion of rooting or growth of semen trigonellae tissue culture seedlings

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Experimental program
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Embodiment 1

[0025] Preparation of Preliminary Example 1 Bacillus licheniformis Bacterial Liquid

[0026] 1) Open the capsule shell of Zhengchangsheng (i.e. Bacillus licheniformis capsules, manufacturer: Northeast Pharmaceutical Group Shenyang First Pharmaceutical Co., Ltd.), dip the dry powder of Bacillus licheniformis in it with an inoculation loop, and inoculate it with LB liquid medium Put it into a shaker and shake it for the first time, the shaking time is 2-3 hours, the set temperature is 30°C, and the rotation speed is 180 rpm.

[0027] Wherein, the formula of the LB liquid medium is: yeast extract 5g / L, tryptone 10g / L, sodium chloride 10g / L, pH 6.8-7.2.

[0028] 2) After the first shaking is completed, use an inoculation loop to dip in the bacterial liquid, inoculate it evenly on the LB solid medium, culture it in a constant temperature incubator at 28°C for 48 hours, and take a single colony in the LB liquid medium Put it into a shaker and shake it for the second time, the shaki...

Embodiment 1

[0039] Get the blueberry progeny seedlings (2-3cm) consistent with the growth conditions in Example 1 and soak them in sterile deionized water for 5 minutes (with the same volume as the Bacillus licheniformis diluted in Example 1), and then inoculate them in blueberry sterile water. Carry out tissue culture in the sugar tissue culture medium, and the culture time is 8 weeks to obtain blueberry tissue culture seedlings.

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Abstract

The invention discloses application of bacillus licheniformis to the promotion of rooting or growth of semen trigonellae tissue culture seedlings. The application comprises the bacterium activation of a raw material entrocoor dinatibiogen and inoculation to successive semen trigonellae seedlings in form of bacillus licheniformis liquid, a large number of separation, purification and strain identification steps are eliminated, the addition of any exogenous hormones is avoided, the cost is low, the operation is easy, safe and nontoxic, and remarkable effects are achieved; the rooting rate of the inoculated semen trigonellae tissue culture seedlings is remarkably increased, indexes such as plant height and leaf area are also improved, the survival rate after fungal contamination is greatly increased, and the rooting quality of the semen trigonellae tissue culture seedlings is remarkably improved.

Description

technical field [0001] The invention relates to the application of bacillus licheniformis, in particular to the application of bacillus licheniformis in promoting rooting or growth of plant tissue culture seedlings, and belongs to the application field of bacillus licheniformis. Background technique [0002] Blueberry (Semen Trigonellae) has health effects such as softening human blood vessels and enhancing immune function. It is known as the "King of Fruits" and is also listed as one of the top five health foods for human beings by the Food and Agriculture Organization of the United Nations. The market price is expensive. For now, blueberry production is far from meeting domestic demand, and various processed blueberry products are very popular in the international market. However, due to its limited source of materials and low reproduction coefficient, large-scale production cannot be realized. Tissue culture is an important way to solve this problem. However, the convent...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 姬谦龙贾士乾王贵禧韩素英刘悦萍于涌鲲张乔丽
Owner 贡格尔(北京)农牧科技有限公司
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