Method for tissue culture and rapid propagation of Huzhou lily

A technology of lily tissue culture and lily, which is applied in the field of plant tissue culture, can solve the problems of great difference in technical conditions, increased difficulty of disinfection process, high pollution rate, etc., to improve the survival rate of transplanting, which is beneficial to storage and has a low survival rate Effect

Inactive Publication Date: 2013-05-22
ZHEJIANG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

At present, there have been a lot of studies on the group cultivation of Lily of Lanzhou, Lilium chinensis, Lily of the Wild and Lily of the Oriental line, etc., but the technical conditions required for different species of lily are very different due to the difference in genetic background such as genotype.
However, there is no report on the systematic research on the tissue culture and rapid propagation technology of Huzhou lily. In particular, the bulbs of lily grow underground and have many bacteria, which makes the disinfection process more difficult during the tissue culture and rapid propagation process, and there is always a high pollution rate. The problem

Method used

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  • Method for tissue culture and rapid propagation of Huzhou lily
  • Method for tissue culture and rapid propagation of Huzhou lily
  • Method for tissue culture and rapid propagation of Huzhou lily

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The influence of embodiment 1 different refrigerating time on the sterilizing effect of Huzhou lily scale

[0044] Huzhou lily bulbs were purchased from a farmer in Huzhou, and stored in a refrigerator at 4°C for a period of time (0, 1, and 2 weeks, respectively, see Table 1).

[0045]After removing the dry and infested outer scales of Huzhou lily bulbs and washing them, soak the scales of Huzhou lily in tap water containing detergent for about 30 minutes, rinse them with running water, and disinfect them with 75% alcohol for 30 seconds in an ultra-clean workbench , then soak for 20 minutes in mercuric chloride with a mass volume concentration of 0.1%, rinse with sterile water 3 to 5 times, and set aside.

[0046] The middle and lower parts of the scales after detoxification were cut into uniform small squares of 1cm×1cm with a scalpel, and inoculated on the induction medium (agar, 8gL; sucrose, 30g / L; MS powder, 4~5g / L; 6 -BA, 1.0mg / L; NAA, 0.5mg / L; pH 5.6~5.8), the n...

Embodiment 2

[0052] Example 2 Effects of different disinfectant treatments on the sterilizing effect of Huzhou lily scales

[0053] 1. The effect of alcohol disinfection treatment on the sterilization effect of Huzhou lily scales

[0054] Store Huzhou lily bulbs in a refrigerator at 4°C for 2 weeks.

[0055] After removing the dried and infested outer scales of Huzhou lily bulbs and washing them, soak the scales of Huzhou lily in tap water containing detergent for about 30 minutes, rinse with running water and disinfect with 75% alcohol, and the disinfection time is 0.5 min, 1 min, 3 min, 5 min, rinse 3~5 times with sterile water, and set aside.

[0056] The middle and lower parts of the scales after detoxification were cut into uniform small squares of 1cm×1cm with a scalpel, and inoculated on the induction medium (agar, 8g / L; sucrose, 30g / L; MS powder, 4~5g / L) with the concave side up. ; 6-BA, 1.0mg / L; NAA, 0.5mg / L; pH is 5.6~5.8), the inoculum number of different treatments is 15 expl...

Embodiment 3

[0090] Example 3 Effects of Different Induction Mediums on the Induction Culture of Lily Huzhou

[0091] (1) Disinfection of explants

[0092] Huzhou lily bulbs were purchased from a farmer in Huzhou and stored in a refrigerator at 4°C for 2 weeks.

[0093]After removing the dry and infested outer scales of Huzhou lily bulbs and washing them, soak them in tap water containing detergent for about 30 minutes, and then use a three-step disinfection method after rinsing with running water. The first step is to use 250mg / L methyl alcohol Shake the thiophanate solution at room temperature for 0.3h, the second step is to sterilize with 75% alcohol on the ultra-clean workbench for 30s, and the third step is to contain 0.1% Tween-20 by volume, and the mass volume concentration is 0.1%. Soak in mercury for 20 minutes, rinse with sterile water 3 to 5 times, and set aside.

[0094] (2) Take the middle and lower part of the scales and use a scalpel to cut them into uniform small squares ...

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Abstract

The invention discloses a method for tissue culture and rapid propagation of Huzhou lily. The method comprises the following steps of: putting cleaned Huzhou lily scales into thiophanate methyl of which the concentration is 200-300mg / L, oscillating for 0.3 to 1 hour, soaking in 70-75% alcohol for 20 to 30 seconds, soaking in corrosive sublimate of which the mass volume concentration is 0.1-0.2% for 15 to 25 minutes, and rinsing out the scales; inoculating the rinsed-out scales to an induced medium to perform induced culture; after induced spouting, migrating sprouts into an enrichment medium to perform enrichment culture so as to obtain cluster buds; and inoculating the cluster buds to a strong-ball rooting medium to obtain aseptic seedlings. By adopting the method, the problem that explants of the Huzhou lily are easily subjected to fungal contamination and bacteria contamination during primary culture is solved, the contamination rate is controlled to be below 25%, and the death rate is low; and moreover, a wholes set of complete processes of primary culture, secondary culture, strong-ball culture and rooting culture is established and has high induction rate and multiplication multiple.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Huzhou lily. Background technique [0002] Edible lily is a safe and non-toxic food that can be eaten after many years of domestication, variety selection and artificial cultivation of wild lily. [0003] Edible lily can be divided into two categories: sweet lily and bitter lily. Lanzhou lily, dragon tooth lily, lily lily and Sichuan lily are the main edible lilies in China. Lilium lily, also known as tiger skin lily and Nanjing lily, belongs to the bitter lily class of edible lily. The wild species are widely distributed in my country, and the cultivation purposes for food are mainly concentrated in Jiangsu and Zhejiang regions. [0004] Huzhou lily (Lilium lancifolium) is an ecological type of lily lily. It has been cultivated for five to six hundred years. It is mainly distributed in Taihu Lake, Yangxi, Tangdia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 吴昀常乐夏宜平张琳马怡迪张佳平
Owner ZHEJIANG UNIV
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