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A tissue culture, chrysanthemum technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effects of wide nutrient sources, inhibition of the growth of miscellaneous bacteria, and easy cultivation and growth
Active Publication Date: 2022-03-22
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology
At present, there are many studies on tissue culture and rapid propagation of chrysanthemum seedlings, and the technical system is becoming more and more perfect, but most of them are mainly aimed at improving production efficiency and reducing the pollution of tissue culture seedlings. Research on property varieties has not been reported
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Embodiment 1
[0027] Example 1 Bacteriostat Screening and Culture Medium Preparation
[0028] Screening of bacteriostatic agents: ε-polylysine, isothiazolinone, streptomycin, and chlorothalonil were selected in advance by the Key Laboratory of Crop Cultivation and Germplasm Innovation of Nanjing Agricultural University and could be used for antibacterial culture of chrysanthemums. Bacteriostats, mixed in equal amounts 1:1:1:1 to prepare MS medium containing different concentrations of bacteriostats, the concentrations are 0.025g / L, 0.05g / L, 0.075g / L L, 0.1g / L, the prepared medium is divided into glass tissue culture bottles, 40ML per bottle, sterilized in a sterilizing pot at 121°C for 30min, cooled and solidified for later use. Select Penicillium griseoviridum, inoculate it in 1 / 4MS liquid medium, culture it on a shaker at 25°C and 20r / min until the OD value is 0.6-0.8, dilute it 10000 times to make a suspension (this concentration can ensure that There is the existence of Penicillium cin...
Embodiment 2
[0031] A tissue culture method for protecting the intellectual property rights of chrysanthemums, mainly aimed at tissue culture and propagation of existing tissue culture seedlings, using a special antibacterial medium and tissue culture method, specifically including the following steps:
[0032] 1) Medium formula
[0033] Antibacterial medium preparation: ε-polylysine 0.05g / L, isothiazolone 0.05g / L, streptomycin 0.05g / L, chlorothalonil 0.05g / L, sucrose 30g / L, agar 7g / L L, MS powder 4.74g / L, the pH of the medium adjusted by NaOH is 5.8-7.2. The prepared medium was divided into glass tissue culture bottles with a volume of 40mL / bottle, sterilized in a sterilizing pot at 121°C for 30min, cooled and solidified for later use.
[0034] 2) Propagation of chrysanthemum intellectual property varieties
[0035] Take the middle and upper young stems of the aseptic seedlings of chrysanthemum intellectual property varieties that need to be protected, inoculate them in ordinary medium ...
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Abstract
The invention discloses a chrysanthemum tissue culture method. The present invention comprises preparation of a bacteriostatic culture medium for a combination of various bacteriostatic agents, and inoculates the Penicillium grisea viridifera that exists widely in nature and is easy to extract, so that the Penicillium grisea viridiens is in a latent state under the action of the bacteriostatic agents, and It does not cause fungal contamination, and the growth and development status of the chrysanthemum tissue culture seedlings cultivated in this medium and the performance after hardening are all normal, and has no effect on the yield and quality of chrysanthemum seedlings; the chrysanthemum group in the antibacterial medium The cultured seedlings were inoculated into ordinary culture medium, and there was no inhibitory effect of bacteriostatic agents. The tissue cultured seedlings broke out fungal contamination in a large area and the contamination rate reached 100%. Through this method, while ensuring the production efficiency and quality of chrysanthemum tissue culture seedlings, it can effectively protect the intellectual property varieties of chrysanthemums. Only units with the right to grant varieties can obtain healthy tissue culture seedlings to prevent the illegal outflow of fine varieties.
Description
technical field [0001] The invention relates to the technical field of tissue culture, in particular to a chrysanthemum tissue culture method. Background technique [0002] Chrysanthemum, also known as Jinying, Jiuhua, Juhua, Jiehua, and Nuhua, is a perennial herbaceous plant of the genus Chrysanthemum in the family Compositae. It is one of the top ten traditional famous flowers in my country and one of the four major cut flowers in the world. It has a long history of cultivation in my country. Historically, it is one of the most abundant ornamental plants. It has a variety of application forms and a wide range of uses, mainly for ornamental, edible, tea and medicinal purposes. In production, chrysanthemum is usually propagated by two methods of cutting and division, but for the large-scale seedling production of new varieties, especially for professional breeding units, the propagation rate of these two methods is far from meeting the market demand. In contrast, tissue cul...
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