Filtering and separating device for stem cells

Abstract

A filtering and separating device for stem cells relates to the technical field of filtering devices and comprises a liquid storage cylinder, a bottom plate is fixedly connected to a lower end opening of the liquid storage cylinder, a plurality of liquid outlet holes are formed in the periphery of the side wall, close to the lower end opening, of the liquid storage cylinder, and a filtering cylinder in frictional contact with the liquid storage cylinder is sleeved on the outer wall of the liquid storage cylinder and vertically slides. A plurality of annular filter screen areas with gradually increased meshes are arranged on the cylinder wall of the filter cylinder side by side from top to bottom; and a pressurizing plate which is in friction sealing with the inner wall of the liquid storage cylinder is also arranged in the liquid storage cylinder in a vertical lifting manner. The utility model solves the problems that when a stem cell filtering device in the prior art is used, different filtering accuracies cannot be adjusted, so that the use range of the stem cell separating device is reduced; the problems that the filtering efficiency is low due to the fact that the stem cell culture solution penetrates through the filtering net under the non-pressure effect, and the penetrating efficiency of the stem cell culture solution is seriously reduced when the filtering net is blocked are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of filtering devices, in particular to a filtering separation device for stem cells. Background Art

[0002] Mesenchymal stem cells are a type of pluripotent stem cells that have all the common properties of stem cells, namely the ability to self-renew and multidirectionally differentiate. They are also the most widely used in clinical applications. When used in combination with hematopoietic stem cells, they can improve the success rate of transplantation and accelerate hematopoietic reconstruction. When patients receive high-dose chemotherapy, the infusion of mesenchymal stem cells together with hematopoietic stem cells can significantly accelerate the patient's blood cell recovery time, and it is safe and has no adverse reactions. Mesenchymal stem cells exist not only in the bone marrow, but also in skeletal muscle, periosteum and trabeculae.

[0003] The existing technology has the following deficiencies: the existing stem cell separation device cannot conveniently adjust the filtering mechanism when separating stem cells, which makes it easy for the raw materials to cause clogging of the filtering mechanism, making it impossible for the filtering mechanism to remain unobstructed during the filtration process, making it impossible for stem cells to be quickly separated from the raw materials, thereby affecting the separation efficiency of stem cells.

[0004] A patent with a publication number of CN216473188U is disclosed in the prior art. The solution includes a device body, and both ends of the inner wall of the device body are provided with limiting grooves. The inner wall of the limiting groove at the top end is provided with a first filter plate, and the inner wall of the limiting groove at the bottom end is provided with a second filter plate. The first filter plate and the second filter plate are provided with an accelerating separation mechanism on the outer sides. The two filter plates can perform secondary filtration on the raw materials, so that parts of different volumes in the raw materials can be respectively retained on the surfaces of the first filter plate and the second filter plate. At the same time, the servo motor works so that the stirring rod can stir the raw materials, so that the raw materials are mixed more fully. At the same time, the rotation of the rotating shaft can drive the two rotating gears to rotate, so that the two filter plates also rotate accordingly, which can avoid clogging of the filter plates by the raw materials, thereby improving the separation efficiency of stem cells.

[0005] As the existing devices including the above patents are used, the shortcomings of the technology are gradually exposed, which are mainly manifested in the following aspects:

[0006] First, the existing stem cell filtering device cannot adjust different filtering accuracies when in use, which reduces the use range of the stem cell separation device.

[0007] Second, when the existing stem cell filtration device is in use, the stem cell culture fluid passes through the filter without pressure, resulting in low filtration efficiency. In particular, when the filter is clogged, the penetration efficiency of the stem cell culture fluid is further reduced.

[0008] In summary, the prior art obviously has inconveniences and defects in practical use, so it is necessary to improve it. Utility Model Content

[0009] In view of the defects in the prior art, the utility model solves the problems that the stem cell filtration device in the traditional technology cannot adjust different filtration accuracies when in use, which reduces the use range of the stem cell separation device; and the stem cell culture fluid passes through the filter without pressure, resulting in low filtration efficiency. In particular, when the filter is blocked, the penetration efficiency of the stem cell culture fluid is more seriously reduced.

[0010] In order to solve the above problems, the utility model provides the following technical solutions:

[0011] A filtration separation device for stem cells comprises a liquid storage cylinder, the lower end of the liquid storage cylinder is fixedly connected to a bottom plate, a plurality of liquid outlet holes are arranged on the side wall of the liquid storage cylinder near the lower end, a filter cartridge is mounted on the outer wall of the liquid storage cylinder in frictional contact with the filter cartridge, the filter cartridge is arranged to slide vertically, and a plurality of annular filter screen areas with increasing mesh sizes are arranged in parallel from top to bottom on the cylinder wall of the filter cartridge.

[0012] A pressure plate is also provided in the liquid storage cylinder and is lifted and lowered vertically to be frictionally sealed with the inner wall thereof.

[0013] As an optimized solution, a liquid inlet channel communicating with the inner cavity of the liquid storage cylinder is provided on the pressure plate, and a liquid inlet one-way valve is provided in the liquid inlet channel.

[0014] As an optimized solution, the upper end of the pressure plate is connected to a connecting tube connected to the liquid inlet channel.

[0015] As an optimized solution, guide blocks are fixedly connected to the relative outer walls of the liquid storage cylinder, and guide grooves matching the guide blocks are vertically opened on the relative inner walls of the filter cylinder.

[0016] As an optimized solution, an external thread section is provided on the outer wall of the liquid storage cylinder above the annular filter area, and a driving ring is threadedly connected through the external thread section, and the driving ring is rotatably arranged.

[0017] As an optimized solution, side brackets are fixedly connected to the relative outer walls of the liquid storage cylinder near the upper end, one of the side brackets is fixedly connected to a driving motor, the output shaft of the driving motor is fixedly connected to a gear, and the outer wall of the driving ring is fixedly connected to a gear ring meshing with the gear.

[0018] As an optimized solution, an annular groove is provided on the outer wall of the driving ring, and the gear ring is fixed in the annular groove.

[0019] As an optimized solution, a lifting cylinder is fixedly connected to the side bracket, and the telescopic end of the lifting cylinder is fixedly connected to the upper surface of the pressure plate through a connecting frame.

[0020] As an optimized solution, a liquid collecting guide cylinder is fixedly connected between the two side brackets, and the liquid collecting guide cylinder is located below the liquid storage cylinder.

[0021] As an optimized solution, the upper surface of the bottom plate is concave from the outside to the center, and a discharge valve connected to the liquid storage cylinder is fixedly connected to the center position of the lower surface of the bottom plate.

[0022] Compared with the prior art, the beneficial effects of the utility model are:

[0023] When used for filtering stem cell culture fluid, the driving machine drives the driving ring to rotate, and the driving ring rotates to drive the filter cartridge to rise and fall vertically, and the annular filter area matching the filtration accuracy is raised and lowered to the area where the liquid outlet is located, so as to filter the culture fluid flowing out of the liquid outlet, thereby adjusting different filtration accuracies;

[0024] When the filter cartridge is raised or lowered, the upper and lower edges of the liquid outlet hole will be used to scrape off the impurities adhering to the inner wall of the annular filter area, thus achieving the function of automatic cleaning;

[0025] After the device is used, the discharge valve can be opened to clean and discharge the filtered objects inside, which is convenient for maintenance;

[0026] When adding stem cell culture fluid, the lifting cylinder drives the pressure plate to move to a high position, and uses the connecting tube to connect the material source to introduce the stem cell culture fluid into the liquid storage cylinder. After the addition is completed, the lifting cylinder drives the pressure plate to move downward to pressurize the internal stem cell culture fluid so that it can quickly pass through the annular filter area, greatly improving the filtration efficiency of the stem cell culture fluid. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the specific implementation of the utility model or the technical solution in the prior art, the following is a brief introduction to the drawings required for the specific implementation or the prior art description. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, each element or part is not necessarily drawn according to the actual scale.

[0028] Figure 1 It is a structural schematic diagram of the utility model.

[0029] In the figure: 1-liquid storage cylinder; 2-liquid outlet; 3-filter cylinder; 4-annular filter area; 5-gear; 6-driving ring; 7-toothed ring; 8-guide groove; 9-guide block; 10-driving machine; 11-side bracket; 12-lifting cylinder; 13-connecting frame; 14-pressure plate; 15-liquid inlet channel; 16-check valve; 17-connecting cylinder; 18-bottom plate; 19-discharge valve; 20-liquid collecting guide cylinder. DETAILED DESCRIPTION

[0030] The following embodiments of the technical solution of the utility model are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the utility model, and are therefore only used as examples, and cannot be used to limit the protection scope of the utility model.

[0031] like Figure 1 As shown, the filtration separation device for stem cells comprises a liquid storage cylinder 1, the lower end of the liquid storage cylinder 1 is fixedly connected to a bottom plate 18, a plurality of liquid outlet holes 2 are arranged on the side wall of the liquid storage cylinder 1 near the lower end, a filter cartridge 3 in frictional contact with the liquid storage cylinder 1 is mounted on the outer wall of the liquid storage cylinder 1, the filter cartridge 3 is arranged to slide vertically, and a plurality of annular filter screen areas 4 with increasing mesh sizes are arranged in parallel from top to bottom on the wall of the filter cartridge 3,

[0032] A pressure plate 14 is also provided in the liquid storage cylinder 1 and is lifted and lowered vertically to form a friction seal with the inner wall thereof.

[0033] The pressure plate 14 is provided with a liquid inlet passage 15 which is in communication with the inner cavity of the liquid storage cylinder 1 , and a liquid inlet one-way valve 16 is provided in the liquid inlet passage 15 .

[0034] The upper end of the pressure plate 14 is connected to a connecting tube 17 connected to the liquid inlet channel 15 .

[0035] A guide block 9 is fixedly connected to the relative outer wall of the liquid storage cylinder 1 , and a guide groove 8 matching the guide block 9 is vertically opened on the relative inner wall of the filter cylinder 3 .

[0036] An external thread section is provided on the outer wall of the liquid storage cylinder 1 above the annular filter screen area, and a driving ring 6 is threadedly connected through the external thread section, and the driving ring 6 is rotatably arranged.

[0037] Side brackets 11 are fixedly connected to the opposite outer walls of the liquid storage cylinder 1 near the upper end, and a driving machine 10 is fixedly connected to one of the side brackets 11. The output shaft of the driving machine 10 is fixedly connected to the gear 5, and the outer wall of the driving ring 6 is fixedly connected to a gear ring 7 meshing with the gear 5.

[0038] An annular groove is provided on the outer wall of the driving ring 6 , and the gear ring 7 is fixed in the annular groove to support the driving ring 6 .

[0039] A lifting cylinder 12 is fixedly connected to the side bracket 11 , and a telescopic end of the lifting cylinder 12 is fixedly connected to the upper surface of the pressure plate 14 through a connecting frame 13 .

[0040] A liquid collecting guide tube 20 is also fixedly connected between the two side brackets 11 . The liquid collecting guide tube 20 is located below the liquid storage tube 1 to collect and guide the filtered liquid.

[0041] The upper surface of the bottom plate 18 is concave from the outside to the center, and a discharge valve 19 connected to the liquid storage cylinder 1 is fixedly connected to the center of the lower surface of the bottom plate 18 .

[0042] The working principle of this device is:

[0043] When used for filtering stem cell culture fluid, the driving machine 10 drives the driving ring 6 to rotate, and the driving ring 6 rotates to drive the filter cartridge 3 to rise and fall vertically, and the annular filter area 4 matching the filtering accuracy is lifted and lowered to the area where the liquid outlet hole 2 is located, so as to filter the culture fluid flowing out of the liquid outlet hole 2, thereby adjusting different filtering accuracies;

[0044] When the filter cartridge 3 is raised or lowered, the upper and lower edges of the liquid outlet hole 2 are used to scrape off the impurities adhering to the inner wall of the annular filter screen area 4, thus achieving the function of automatic cleaning;

[0045] After the device is used, the discharge valve 19 can be opened to clean and discharge the filtered objects inside, which is convenient for maintenance;

[0046] When adding stem cell culture fluid, the lifting cylinder 12 drives the pressure plate 14 to move to a high position, and uses the connecting tube 17 to connect the material source to introduce the stem cell culture fluid into the liquid storage cylinder 1. After the addition is completed, the lifting cylinder 12 drives the pressure plate 14 to move downward to pressurize the internal stem cell culture fluid so that it can quickly pass through the annular filter area 4, greatly improving the filtration efficiency of the stem cell culture fluid.

[0047] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the utility model, and they should all be included in the scope of the claims and specification of the utility model.

Claims

1. Filtration separation device for stem cells, Features: The invention comprises a liquid storage cylinder (1), the lower end of the liquid storage cylinder (1) is fixedly connected to a bottom plate (18), a side wall of the liquid storage cylinder (1) close to the lower end is provided with a plurality of liquid outlet holes (2), a filter cylinder (3) is sleeved on the outer wall of the liquid storage cylinder (1) and is in frictional contact with the liquid storage cylinder (1), the filter cylinder (3) is arranged to slide vertically, and a plurality of annular filter screen areas (4) with increasing mesh sizes are arranged in parallel from top to bottom on the cylinder wall of the filter cylinder (3), A pressure plate (14) is also provided in the liquid storage cylinder (1) and is lifted and lowered vertically to form a friction seal with the inner wall thereof.

2. The stem cell filtration separation device according to claim 1, Features: The pressure plate (14) is provided with a liquid inlet channel (15) which is in communication with the inner cavity of the liquid storage cylinder (1), and a liquid inlet one-way valve (16) is provided in the liquid inlet channel (15).

3. The stem cell filtration separation device according to claim 2, Features: The upper end of the pressure plate (14) is connected to a connecting tube (17) connected to the liquid inlet channel (15).

4. The stem cell filtration separation device according to claim 3, Features: A guide block (9) is fixedly connected to the relative outer wall of the liquid storage cylinder (1), and a guide groove (8) matching the guide block (9) is vertically opened on the relative inner wall of the filter cylinder (3).

5. The stem cell filtration separation device according to claim 4, Features: The liquid storage cylinder (1) is provided with an external thread section on its outer wall above the annular filter screen area, and is threadedly connected to a drive ring (6) via the external thread section, and the drive ring (6) is rotatably arranged.

6. The stem cell filtration separation device according to claim 5, Features: Side brackets (11) are fixedly connected to opposite outer walls of the liquid storage cylinder (1) near the upper end, one of the side brackets (11) is fixedly connected to a driving machine (10), an output shaft of the driving machine (10) is fixedly connected to a gear (5), and a gear ring (7) meshing with the gear (5) is fixedly connected to the outer wall of the driving ring (6).

7. The stem cell filtration separation device according to claim 6, Features: An annular groove is provided on the outer wall of the driving ring (6), and the gear ring (7) is fixedly connected in the annular groove.

8. The stem cell filtration separation device according to claim 7, Features: A lifting cylinder (12) is fixedly connected to the side bracket (11), and the telescopic end of the lifting cylinder (12) is fixedly connected to the upper surface of the pressure plate (14) via a connecting frame (13).

9. The stem cell filtration separation device according to claim 8, Features: A liquid collecting guide tube (20) is also fixedly connected between the two side brackets (11), and the liquid collecting guide tube (20) is located below the liquid storage tube (1).

10. The stem cell filtration separation device according to claim 9, Features: The upper surface of the bottom plate (18) is arranged to be concave from the outside to the center, and a discharge valve (19) connected to the liquid storage cylinder (1) is fixedly connected to the center of the lower surface of the bottom plate (18).

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