Lactobacillus acidophilus and application thereof and feed additive thereof and premix compound thereof

A technology of Lactobacillus acidophilus and feed additives, applied in the biological field, can solve problems such as decreased immunity, enhanced drug resistance of bacteria, and imbalance of animal intestinal flora, and achieves strong stress resistance, reduced antibiotic addition, and active The effect of high bacterial content

Active Publication Date: 2012-07-11
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term and large-scale use of antibiotics in the current animal breeding process, the drug resistance of pathogenic bacteria has increased, the intestinal flora of animals has become imbalanced, and immunity has decreased.

Method used

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  • Lactobacillus acidophilus and application thereof and feed additive thereof and premix compound thereof
  • Lactobacillus acidophilus and application thereof and feed additive thereof and premix compound thereof
  • Lactobacillus acidophilus and application thereof and feed additive thereof and premix compound thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening and identification of Lactobacillus acidophilus CGMCC No.5093

[0024] 1. Primary screening

[0025] Take 5g of fresh pig feces samples, soak them in 20ml of sterile 0.85% normal saline, shake the samples to break up the samples, make the samples fully contact with the normal saline, add the centrifuged bacteria sediment into 10ml of artificial gastric juice, and let it stand at 37°C for 2h , and then centrifuged to collect the bacteria, add the precipitate of the bacteria to 10ml of artificial bile salt, let stand at 37°C for 4 hours, collect the bacteria, dilute and spread on LB medium. After culturing in a 37°C incubator for 12-24 hours, the colonies were purified and isolated. The obtained bacteria were preserved and further re-screened.

[0026] Colony morphology: the colony is round, smooth, opaque, milky white, with smooth edges, Gram-positive bacteria; observed under a microscope after Gram staining, a single bacterium is slender rod-shaped...

Embodiment 2

[0032] Example 2 Experiments on the probiotic function and stress resistance of Lactobacillus acidophilus CGMCC No.5093

[0033] The Lactobacillus acidophilus CGMCC No.5093 obtained by using MRS medium was tested for acid resistance, bile salt resistance and antibacterial activity.

[0034] (1) Acid resistance test of Lactobacillus acidophilus

[0035] Preparation of artificial gastric juice: The preparation method of artificial gastric juice is: 1% pepsin, 0.85% sodium chloride, adjust the pH value to 2.0, 3.0, 4.0 with concentrated hydrochloric acid, filter and sterilize through a bacterial filter for later use; the test results are shown in the table 1.

[0036] Table 1 Lactobacillus acidophilus acid resistance test results

[0037]

[0038] (2) Lactobacillus acidophilus bile resistance liquid test: the preparation method of artificial bile salt is: respectively add 0.1%, 0.2%, 0.3% pig bile salt (analytical pure) in liquid broth, 115 ℃ autoclaving backup used; the te...

Embodiment 3

[0046] The preparation of embodiment 3 Lactobacillus acidophilus CGMCC No.5093 feed additive

[0047] 1) Plate culture rejuvenation: Inoculate Lactobacillus acidophilus strains on the MRS plate medium, culture at 37°C for 24 hours, rejuvenate Lactobacillus acidophilus, and form a single colony, pick a single colony on the inoculation medium, 37 Cultivate for 24 hours at ℃;

[0048] 2) Preparation of primary seeds: transfer the Lactobacillus acidophilus cultured in step (1) into a 1L shaker flask filled with 300ml of MRS liquid medium, and culture at 37°C for 24 hours until the late logarithmic period to obtain primary seeds ;

[0049] 3) Preparation of secondary seeds: transfer the primary seeds prepared in step 2) to a 100L seed tank equipped with 80LMRS seed medium, at a temperature of 37°C, turn 60rpm, stir once every 45 minutes, and each stirring time is 15 minutes, cultivated for 20 hours, and obtained the secondary seed solution.

[0050] 4) Preparation of Lactobacill...

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Abstract

The invention belongs to the field of biotechnology, and relates to Lactobacillus acidophilus and application thereof and a feed additive thereof and a premix compound thereof. The Lactobacillus acidophilus of the invention has strong stress tolerance, and can tolerate simulated gastric fluid and simulated bile salt so as to guarantee that a great quantity of viable bacteria can smoothly enter the intestinal tract of an animal, and the viable bacteria play a probiotic role, so that the immunity of the animal is improved, and the breeding cost is reduced. The product obtained from the Lactobacillus acidophilus of the invention through culture, high-density fermentation and addition of protectant has a high content of viable Lactobacillus acidophilus, can be added as a biological feed additive in the drinking water or feed for breeding animals, and can be prepared into feed such as premix compound and so on, so as to reduce the addition of antibiotics, indeed improve the intestinal tract health and the immunity of animals, and increase the production performance of animals.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a strain of Lactobacillus acidophilus and its application, feed additive and premix. Background technique [0002] Various bacteria inhabit the intestinal tract of animals, which can be divided into beneficial bacteria, neutral bacteria and pathogenic bacteria according to their interaction with the host. In the intestinal tract of healthy animals, various flora maintain a relatively stable state, but when certain environments change, the pathogenic bacteria in the intestinal tract will multiply and produce bacterial toxins at the same time, causing animal diseases, such as bacillary dysentery, etc. . [0003] Lactobacillus acidophilus (Lactobacillus acidophilus) is one of the resident flora in the intestinal tract of animals, which can exist in the gastric juice and secrete lactic acid, acetic acid, etc. to promote the digestive capacity of the stomach; in addition, Lactobacillus aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/23
Inventor 付维来杜建涛王凡王安如王松王丽英
Owner BEIJING DABEINONG TECH GRP CO LTD
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