Thin-shell pecan tissue culture bud micro grafting method

Abstract

The invention discloses a thin-shell pecan tissue culture bud micro grafting method which comprises the following steps of: 1. inducing and multiplying a scion tissue culture bud, wherein five working procedures of collecting and disinfecting an explant, preparing an adventitious bud inducing culture medium, inducing an adventitious bud, preparing an adventitious bud multiplying culture medium and performing adventitious bud multiplying cultivation; 2. cultivating a thin-shell pecan stock; 3. grafting the tissue culture bud; and 4. managing grafting and a healing stage. According to the method, the thin-shell pecan has the advantages of retaining the maternal characteristics along with early bearing, being high in inversion resistance, high in multiplying speed and the like.

Description

technical field [0001] The invention relates to a micro-grafting method for grafting adventitious buds cultivated by tissue cultivation of pecan to the new shoots of annual seedlings of pecan. Background technique [0002] Thin shell hickory [Carya illinoinensis (Wangehn.) K.Koch.], also known as American hickory, long hickory, is a deciduous tree of the family Juglandaceae Hickory, native to the southern United States and northern Mexico, and is a famous oil-bearing dried fruit in the world One of the tree species, it is also an excellent economic tree species for both fruit and wood, and can adapt to a wide range of soil pH. In the natural environment, pecans are propagated by seeds. Dried pecans with thin shells can be processed into various special flavor foods and health products. The oil content of the kernels is as high as 70% to 72%, rich in unsaturated fatty acids, 7.8% to 9.6% of crude protein and K, Ca, Mg , Na and other mineral elements necessary for the human b...

AI Technical Summary

Problems solved by technology

After retrieval, no related reports have been found so far on the method of cultivating adventitious buds in test tubes, micro-grafting, propagation, rooting, and planting through the annual branches of the thin-shelled hickory tree.