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Breeding method of induction line for efficiently identifying corn haploid young embryo

A haploid induction system, haploid immature embryo technology, applied in the direction of botanical equipment and methods, biochemical equipment and methods, microbial measurement/testing, etc., can solve the problem of limiting haploid tissue culture identification and doubling Efficiency, large differences in induction systems, etc.

Active Publication Date: 2020-05-19
CHINA AGRI UNIV
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Problems solved by technology

Therefore, when the existing induction lines are directly used to identify haploid immature embryos, the differences between the various induction lines are large, which greatly limits the efficiency of haploid tissue culture identification and doubling
At present, there are no reports on the selection of induced lines specifically for the identification of haploid immature embryos

Method used

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  • Breeding method of induction line for efficiently identifying corn haploid young embryo
  • Breeding method of induction line for efficiently identifying corn haploid young embryo
  • Breeding method of induction line for efficiently identifying corn haploid young embryo

Examples

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Embodiment 1

[0083] Example 1. Breeding and application of an induction line for efficient identification of maize haploid immature embryos

[0084] 1. Breeding of induction lines for efficient identification of maize haploid immature embryos

[0085] Schematic diagram of breeding induced lines for efficient identification of maize haploid immature embryos image 3 .

[0086] 1. In the spring of 2015, the maize haploid induction line (CAU3 or CAU5) was used as the male parent and S23 was used as the female parent to obtain hybrid F1.

[0087] The hybridization method is: field planting (experimental site is Beijing) maize haploid induction line (as the male parent) and S23 (as the female parent), and before the filaments of the female parent are spit out, they are subjected to emasculation and ear bagging; After the filaments are spit out, cut the filaments and pollinate.

[0088] 2. In the winter of 2015, hybrid F1 was planted in the field (the experimental site was Hainan), and backcr...

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Abstract

The invention discloses a breeding method of an induction line for efficiently identifying a corn haploid young embryo. The method comprises the following steps of hybridizing a corn haploid inductionline with a corn inbred line S23 to obtain a hybrid F<1> generation; backcrossing the hybrid F<1> generation and the corn haploid induction line to obtain a backcross posterity BC<1>F<1>; selecting asingle plant of the corn from the backcross posterity BC<1>F<1> to continuously self-cross to obtain the induction line for efficiently identifying the corn haploid young embryo, wherein the single plant of the corn is high in color developing degree of the young embryo after being hybridized with corn hybrids, high in haploid induction rate and high single-ear haploid number after being hybridized with the corn hybrids and is homozygous for both Zmpla1 gene and Zmdmp gene; and the haploid inductivity, the single-ear haploid number, the plant height, the ear position, the tassel branch number, the single-ear fructification number and the haploid young embryo identification accuracy of the induction line for efficiently identifying the corn haploid young embryo are all higher than those ofthe corn haploid induction line. The breeding method of the induction line for efficiently identifying the corn haploid young embryo has important application value.

Description

technical field [0001] The invention belongs to the field of biological breeding, in particular to a breeding method for efficiently identifying induced lines of maize haploid immature embryos. Background technique [0002] Maize haploid breeding technology has been widely used in commercial maize breeding. It mainly includes three aspects of haploid induction, identification and doubling. As an important first step in haploid breeding technology, haploid induction promotes the continuous development of haploid parthenogenic induction lines. The existing induction lines are all derived from Stock6, and its induction rate is 3.2% (Coe, 1959). With the artificial test breeding and molecular marker-assisted selection, a large number of high-frequency induction lines of maize haploid have been bred, mainly including the CAU series , CHOI series, UH series, RWS / K series, TAIL series and PHI series (Ren et al, 2017), the haploid induction rate increased by about 10%. The contin...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H1/02C12Q1/6895
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/172
Inventor 陈绍江陈琛肖子健刘晨旭张俊稳李伟李金龙
Owner CHINA AGRI UNIV
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