A method for efficiently identifying induced lines of maize haploid immature embryos

A haploid induction line, haploid immature embryo technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, microbial assay/inspection, etc., can solve the problems that limit the identification and doubling of haploid tissue culture. Efficiency, large differences in induced lines, etc., achieve the effects of important application value, short breeding cycle, and delayed powder dispersion time.

Active Publication Date: 2021-10-22
CHINA AGRI UNIV
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Problems solved by technology

Therefore, when the existing induction lines are directly used to identify haploid immature embryos, the differences between the various induction lines are large, which greatly limits the efficiency of haploid tissue culture identification and doubling
At present, there are no reports on the selection of induced lines specifically for the identification of haploid immature embryos

Method used

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  • A method for efficiently identifying induced lines of maize haploid immature embryos
  • A method for efficiently identifying induced lines of maize haploid immature embryos
  • A method for efficiently identifying induced lines of maize haploid immature embryos

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Embodiment 1

[0083] Example 1. Breeding and application of an induction line for efficient identification of maize haploid immature embryos

[0084] 1. Breeding of induction lines for efficient identification of maize haploid immature embryos

[0085] Schematic diagram of breeding induced lines for efficient identification of maize haploid immature embryos image 3 .

[0086] 1. In the spring of 2015, the maize haploid induction line (CAU3 or CAU5) was used as the male parent and S23 was used as the female parent to obtain hybrid F1.

[0087] The hybridization method is: field planting (experimental site is Beijing) maize haploid induction line (as the male parent) and S23 (as the female parent), and before the filaments of the female parent are spit out, they are subjected to emasculation and ear bagging; After the filaments are spit out, cut the filaments and pollinate.

[0088] 2. In the winter of 2015, hybrid F1 was planted in the field (the experimental site was Hainan), and backcr...

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Abstract

The invention discloses a breeding method for efficiently identifying induced lines of maize haploid immature embryos. The method comprises the steps of: crossing a corn haploid inducible line with a corn inbred line S23 to obtain a hybrid F 1 generation; hybrid F 1 Generation and maize haploid induction line backcross, get backcross offspring BC 1 f 1 ; in the backcross offspring BC 1 f 1 Among them, select "after crossing with corn hybrids, the degree of immature embryo color development is high", "after crossing with corn hybrids, the haploid induction rate is high and the number of haploids per ear is high" and "Zmpla1 gene and Zmdmp gene are both pure Continuous selfing of single maize plants to obtain an inducible line for efficient identification of haploid immature embryos in maize; haploid induction rate, number of haploids per ear, and strain The height, ear position, number of tassel branches, number of seeds per ear and identification accuracy of haploid immature embryos were all higher than those of maize haploid induced lines. The invention has important application value.

Description

technical field [0001] The invention belongs to the field of biological breeding, in particular to a breeding method for efficiently identifying induced lines of maize haploid immature embryos. Background technique [0002] Maize haploid breeding technology has been widely used in commercial maize breeding. It mainly includes three aspects of haploid induction, identification and doubling. As an important first step in haploid breeding technology, haploid induction promotes the continuous development of haploid parthenogenic induction lines. The existing induction lines are all derived from Stock6, and its induction rate is 3.2% (Coe, 1959). With the artificial test breeding and molecular marker-assisted selection, a large number of high-frequency induction lines of maize haploid have been bred, mainly including the CAU series , CHOI series, UH series, RWS / K series, TAIL series and PHI series (Ren et al, 2017), the haploid induction rate increased by about 10%. The contin...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04A01H1/02C12Q1/6895
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/172
Inventor 陈绍江陈琛肖子健刘晨旭张俊稳李伟李金龙
Owner CHINA AGRI UNIV
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