Technique for extracting grease from oil-tea camellia seed by enzyme method

A technology of enzymatic extraction and camellia oleifera seed, which is applied in the field of enzymatic extraction of oil from camellia oleifera seed, can solve problems such as unfavorable process operation, damage to cell structure, emulsification, etc., to reduce process links, increase oil yield, The effect of preventing degradation

Inactive Publication Date: 2008-08-06
江西春源绿色食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tea saponin and protein are substances with strong emulsification and foaming properties. If the cell structure is destroye

Method used

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  • Technique for extracting grease from oil-tea camellia seed by enzyme method

Examples

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Effect test

example 1

[0032] Take 1kg of air-dried tea oil seed kernels, crush them through a 100-mesh sieve, add 3L of ordinary pure water, soak at 90°C for 30 minutes, add 0.4g of acid protease, extract at 50°C for 4 hours, centrifuge at 4000 rpm for 30 minutes, from top to bottom Divided into free oil layer, emulsifiable oil layer, water layer and residue layer; take out the free oil layer, heat treat the emulsifiable oil layer at 90°C for 10 minutes, centrifuge at 5000 rpm for 30 minutes, take the upper oil layer and combine it with the clean oil layer to obtain a clean oil of about 250 grams.

example 2

[0034] Take 1.5kg of air-dried Camellia oleifera seeds, crush them through a 100-mesh sieve, add 5L of ordinary pure water, soak at 90°C for 30 minutes, add 0.6g of acid protease / vitaminase (3:1), and extract at a constant speed of 50°C for 4 hours , centrifuge at 4000 rpm for 30 minutes, and divide it into free oil layer, emulsifiable oil layer, water layer and residue layer from top to bottom; take out the net oil layer, freeze the emulsifiable oil layer at -20°C, then thaw at 90°C for 10 minutes, Centrifuge for 30 minutes, let it stand, take the upper oil layer and combine it with the net oil layer to obtain about 370 grams of net oil.

example 3

[0036] Take 1.5kg of air-dried Camellia oleifera seeds, crush them through a 100-mesh sieve, add 5L of ordinary pure water, soak at 90°C for 30 minutes, add 0.6g of acid protease / vitaminase (1:1), and extract at a constant speed of 50°C for 4 hours , centrifuge at 4000 rpm for 30 minutes, and divide it into free oil layer, emulsifiable oil layer, water layer and residue layer from top to bottom; take out the clean oil layer, treat the emulsifiable oil layer at 90°C for 10 minutes, centrifuge at 5000 rpm for 30 minutes, and let it stand. Take the upper oil layer and combine it with the net oil layer to obtain about 370 grams of net oil.

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Abstract

The invention relates to an enzymatical extraction process of oil in tea seed oil kernel, which comprises the following steps: firstly, utilizing dried tea seed oil kernel to be raw material, immersing in water in high temperature after disintegrating, stirring in uniform speed under 70-100 DEG C, secondly, enzyme hydrolyzing, adding acid protease which is 0.02-1% dry weight of disintegration samples or mixed enzyme of acid protease/cellulose in 45-55 DEG C to stir in uniform speed, and enzymatic hydrolysis for 2-4 hours, thirdly, separation emulsion breaking processing, directly separating out primary free boiled oil after enzymolysis liquid is centrifuged, getting secondary free boiled oil through centrifuging after missible oil demulsifies, combining the primary free boiled oil and the secondary free boiled oil, and getting the total boiled oil. The invention has the advantages that first, the invention reduces the problem of emulsification, foaming and the like which are faced when a grinding method is utilized to destroy cell structures, second, the invention prevents various enzymes in cells from degrading oil, which has high oil extraction rate to a further disintegrated function to tea seed oil kernel cell, third, the invention increases the treatment to missible oil, which increases free boiled oil yield through demulsifying, centrifuging, stewing to layer and the other modes.

Description

technical field [0001] The invention relates to the application of enzyme preparation in the field of food, especially in the technical field of vegetable oil extraction, and mainly relates to an enzymatic extraction process of oil in camellia oleifera seed kernels. Background technique [0002] Camellia oleifera is an excellent oil tree species unique to my country. The oil content of Camellia oleifera seeds is generally higher than 30%, and the protein content is 10%, and the cellulose content is 7-8%. Its oil contains palmitic acid, oleic acid, linoleic acid, linolenic acid and other fatty acids, of which the oleic acid content is as high as 75%, the total unsaturated fatty acid content is more than 90%, and is also rich in VE, phytosterol and other nutrients. Its excellent quality is deeply loved by people. It is a high-grade edible oil with broad market prospects. [0003] Camellia oil extraction currently adopts the same process as other bulk oil crops, mainly by pr...

Claims

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Application Information

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IPC IPC(8): C12P7/64
Inventor 方学智姚小华王开良王亚萍任华东李生
Owner 江西春源绿色食品有限公司
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