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Method for selecting cabbage-type rape varieties and materials by rape double haploid inducing line

A technology of cabbage-type rape and double haploids, which is applied in horticultural methods, botanical equipment and methods, plant gene improvement, etc., and can solve the problems of low induction efficiency, etc.

Active Publication Date: 2016-11-09
CHENGDU ACAD OF AGRI & FORESTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The earliest maize haploid induction line is stock6, which can only induce haploids in maize, and then the haploid plants undergo artificial chromosome doubling to form homozygous diploids (double haploids), and the induction efficiency Low, generally the induction efficiency is below 10% (calculated based on the number of haploids obtained from harvested seeds)

Method used

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  • Method for selecting cabbage-type rape varieties and materials by rape double haploid inducing line
  • Method for selecting cabbage-type rape varieties and materials by rape double haploid inducing line
  • Method for selecting cabbage-type rape varieties and materials by rape double haploid inducing line

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Experimental program
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Effect test

Embodiment 1

[0085] see figure 1 , figure 2 , Figure 5 , Figure 7 , the cabbage-type rapeseed Ya'an Brassica napus YH (Sichuan Ya'an local variety, diploid, 2n=20) has the advantages of high oil content, yellow seeds, short growth period, early maturity, and tolerance to poor soil, but self-incompatibility cannot form Stable hereditary strains can only be achieved through population improvement to achieve stable heredity. Therefore, the use in production has certain limitations. In order to obtain stable genetic YH strains, the non-stable YH is used as the female parent, and the rapeseed double haploid induction line Y4958 obtained by the applicant is used as the male parent for pollination induction. f 1 The generation (induced offspring) was planted as a single plant, and the flow cytometry test was carried out. The selection was normal, the ploidy (diploid) was normal, and there was no dominant trait (mosaic) of the induced line. The stigma was treated with normal saline and fo...

Embodiment 2

[0165] see figure 1 , figure 2 , Figure 4 , Figure 8 ,Cabbage-type rape Ya'an butter rape YH (Sichuan Ya'an local variety, diploid, 2n=20) has the advantages of high oil content, yellow seeds, short growth period, early maturity, and tolerance to poor soil, but the quality is high in erucic acid and high in sulfur Glycosides, poor disease resistance, easy lodging and other shortcomings; Brassica napus 08nl introduced from Gansu, has the advantages of drought resistance, cold resistance, lodging resistance, double low quality (low erucic acid, low glucosides), good disease resistance, etc. In order to obtain the new Brassica napus which share the advantages of Ya'an oilseed rape and 08nl Brassica napus, YH and 08nl were crossed, and F 1 At the bud moss stage, it is artificially detasselled and isolated by bagging. The double haploid induced line Y3380 obtained by the applicant is used for pollination and bagged for isolation. induced progeny F 2 Planted as a single pla...

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Abstract

A method for selecting cabbage-type rape varieties and materials by a rape double haploid inducing line includes 1), determining target traits of breeding of cabbage-type rape varieties and materials; 2), performing crossing, convergent crossing or backcrossing on at least two cabbage-type rapes with the target traits; 3), pollinating cross, convergent cross or backcross descendants by the rape double haploid inducing line; 4), inducing the descendants to overcome self-incompatibility, and verifying genetic stability of the induced descendants; 5), verifying yield, resistance and quality characters of the induced stable descendants; 6), according to the yield, the quality characters and the resistance, determining stable strains to form conventional varieties of the cabbage-type rapes; 7), inducing the genetic stable strains of the descendants to form a new variety line of the cabbage-type rapes, and test-crossing the new variety line with a sterile line to form a maintainer line or restorer line, or entering a next circle of variety and material breeding process. By the method, breeding speed and efficiency of hybrid or conventional varieties of the cabbage-type rapes can be increased greatly, and manpower and material resources can be reduced.

Description

Technical field: [0001] The invention is related to agriculture, in particular to a method for breeding Chinese cabbage-type rape varieties and materials with rapeseed double haploid induction lines. Background technique: [0002] Rapeseed is the main oil crop in my country, including 3 cultivars, Brassica napus ( Brassica napus , a compound species evolved from Brassica oleracea (aa, n=10) and Brassica cabbage (cc, n=9) through natural interspecific hybridization and double diploidization. According to the source of chromosomes, it is tetraploid, 2n=38 ); Brassica juncea, a compound species evolved from Brassica juncea (aa, n=10) and black mustard (bb, n=8) through natural interspecific hybridization and double diploidization, according to the chromosome The source was judged to be tetraploid, 2n=36). Brassica napus ( Brassia campestris L. Including Brassica and Chinese cabbage native to China. China is also known as Chinese cabbage, dwarf rape, sweet rape and so on. ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H4/00
CPCA01H1/02A01H1/04A01H4/001
Inventor 付绍红杨进王继胜李云邹琼陶兰蓉康津明唐蓉
Owner CHENGDU ACAD OF AGRI & FORESTRY SCI
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