Method for selecting and cultivating hybridized rice infertility series resisting ear germination using molecule marker

Abstract

The invention relates to a method to cultivate preharvest sprouting resistance hybrid rice sterile line by molecular marker-assisted selection. The steps of the invention are that: step A, hybridization, backcrossing and self crossing are performed by using a preharvest sprouting resistance variety as donor parent and the corresponding maintainer line of sterile line having high combining ability but with easy pre-harvest sprouting as recurrent parent; meanwhile the donor parent and the recurrent parent are planted at every season; when choosing among various segregating generations, two microsatellite labeled primers RM447 and RM3754 are used to introduce molecular marker-assisted selection; a plurality of key strains are acquired after two or three generations of backcrossing and five to seven generations of self crossing; step B, the acquired key strains of step A and the sterile line having high combining ability but with easy pre-harvest sprouting are backcrossed for four to seven generations in pairs; during the backcrossing process, the same method of molecular marker-assisted selection as step A is used in selection, then the preharvest sprouting resistance hybrid rice sterile line is cultivated. The invention has the advantages that preharvest sprouting resistance material can be fast and effectively selected; the breeding scale is reduced; the breeding cost is lowered; the preharvest sprouting resistance hybrid rice sterile line is fast selected and bred with high efficiency.

Description

technical field [0001] The invention belongs to the field of rice molecular breeding, and in particular relates to a method for cultivating panicle germination-resistant hybrid rice sterile lines by means of molecular marker-assisted selection. Background technique [0002] Rice is one of the main food crops in my country. The development of rice production is of great significance to ensure food security, maintain social stability, and promote economic growth. [0003] Spike germination is an important agronomic trait related to rice quality and rice seed quality. In the process of CMS reproduction and seed production in the southern rice regions of my country, due to the wide use of gibberellins and the high temperature and humidity in the harvest season, the ear germination rate is usually 20% to 30%, and the highest can reach 60% to 80%. %, seriously affecting the quality of hybrid rice seeds. [0004] Panicle germination is closely related to rice dormancy. It is a qu...

AI Technical Summary

Problems solved by technology

However, the quantitative trait loci detected in these studies have little effect on the interpretation of phenotypic variation and are difficult to use for molecular marker-assisted selection