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Artificial microalgae strain breeding method

A technology for microalgae algae and algae strains is applied in the field of artificial breeding of microalgae algae strains, which can solve the problems of long time and achieve the effects of short processing time, avoiding toxicity or strong cell damage

Inactive Publication Date: 2015-06-17
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] Conventional microalgae strain screening is also a time-consuming task, because most detection methods are based on obtaining a certain amount of biomass
For example, conventional microalgae lipid assessment requires at least hundreds of milliliters of microalgae liquid, and it takes an average of one month of culture time from a solid plate to the required biomass

Method used

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  • Artificial microalgae strain breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Taking Isochrysis zhangjiangensis FACHB-1750, preserved in the Algal Species Preservation Center of Wuhan Institute of Hydrobiology, Chinese Academy of Sciences as the object, the Atmospheric Room Temperature Plasma Breeding Machine (ARTP) jointly developed by the Department of Chemical Engineering and Engineering Physics of Tsinghua University was used. ) for mutagenesis of golden algae.

[0022] 1. Adjust the concentration of the Zhanjiang Isoflagellate culture solution to 1-10 million cells / ml, and take 10-200 microliters of the algae solution and put it on the slide.

[0023] 2. Place the slide on the stage, and adjust the distance between the jet outlet and the slide to be less than or equal to 10mm.

[0024] 3. The output power of the power supply is 50-200W, the working air flow is 1-20SLM, and the discharge time is 5 seconds to 2 minutes.

[0025] 4. Rinse the slide with 800 microliters of golden algae culture solution (natural seawater + F / 2 medium), and recov...

Embodiment 2

[0032] The mutagenesis and other operations are the same as in Example 1, and the screening is carried out with the goal of high temperature resistance, and the coated plates after the mutagenesis are placed at 36°C for cultivation; the light intensity is 30-50 μmol·m -2 ·s -1 , the light-to-dark ratio is 14:10.

[0033] Finally, 9 algae strains with high temperature resistance and high oil content were obtained, and the control was the wild strain under normal culture conditions.

Embodiment 3

[0035] High-yield starch-yielding marine green alga Tetraselmis subcordiformis FACHB-1751, preserved in the Algae Collection Center of Wuhan Institute of Hydrobiology, Chinese Academy of Sciences) strain selection. The mutagenesis was carried out with 1.5 million cells / ml of Tetraspermum algae, the distance between the plasma emission source and the sample was 2mm, the culture medium was natural seawater + Conway nutrient salt, and the mutagenesis time was 30s. After the mutagenesis, dilute the algae solution of Tetragalus 10,000 times, and apply it to the solid plate of Kangweifang. Other operations are the same as in Example 1.

[0036] After mutagenesis, the lethal rate was 99.6%. The starch content of the mutagenized algae strains was investigated by the color value of iodine staining.

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Abstract

The present invention relates to an artificial microalgae strain breeding method, which comprises normal pressure and room temperature plasma mutagenesis of microalgae strains and algae strain screening based on absorbance and specific dye luminescence intensity change. The artificial microalgae strain breeding method is characterized in that the normal pressure and room temperature plasma mutagenesis technology, the microalgae growing testing based on absorbance, and the microalgae grease evaluation method based on the fluorescent dye are combined, and the artificial microalgae strain breeding method has characteristics of efficient and rapid target algae strain obtaining compared with the existing microalgae strain breeding method.

Description

technical field [0001] This patent belongs to the technical field of microalgae cultivation (C12N1 / 12), and is an artificial breeding method for microalgae strains. Background technique [0002] Microalgae are autotrophic microorganisms, also known as microalgae plants because of the photosynthesis system in their cells. Microalgae have the ability to efficiently absorb solar energy and fix carbon dioxide to produce biomass (that is, photosynthetic ability); at the same time, as a kind of microorganism, microalgae proliferates faster. As single-celled organisms, microalgae are mainly composed of protein, fat, carbohydrates and nucleic acids. At present, microalgae has become an important breeding bait and widely used by farming enterprises. At the same time, microalgae has also become a source of health food such as carotenoids, proteins, and polysaccharides. As the prospect of fossil energy depletion intensifies, the potential application of microalgae in energy products ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N15/01C12R1/89
Inventor 薛松艾江宁曹旭鹏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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