Efficient breeding method for families of Portunus trituberculatus

Abstract

The invention discloses an efficient breeding method for families of Portunus trituberculatus and belongs to the field of fishery breeding. According to the method, different wild geographical populations of the Portunus trituberculatus are collected, parent crab mating is completed with a manually controlled directional mating technology, after mating, large pincer muscles of male crabs are taken and stored at the ultralow temperature being subzero 80 DEG C, mated female crabs live through the winter and discharge the young, families are constructed, and after discharging of the young, large pincer muscles of the female crabs are taken and stored at the ultralow temperature being subzero 80 DEG C; when the young of the families develop to young crabs in the stage II, the young crabs are transferred outdoors for breeding, all the families are mixed in the equal proportion for releasing, test populations are established, and meanwhile, a conservation population is established for each family; at the 120-day age, growth traits of each individual of the test populations are measured, then family identification is performed on the individuals by the aid of screened micro-satellite primers, and statistics about survival rates and the growth traits of the families is performed; comprehensive indexes are calculated according to the survival rates and the growth traits of the families, breeding of the families is performed, reservation families are required, individual selection from the families is performed on conservation populations, parent crab mating is completed with an indoor manual directional mating technology, and accurate breeding of the families is realized.

Description

technical field [0001] The invention belongs to the field of aquatic breeding, and in particular relates to a high-efficiency breeding method for Portunus trituberculatus families. Background technique [0002] Portunus trituberculatus (Portunus trituberculatus) is an important marine economic animal, with a current breeding area of ​​more than 400,000 mu and an output of 110,000 tons. It has become one of the leading species of mariculture in my country. At present, the aquaculture industry of Portunus trituberculatus mainly relies on catching wild crabs to meet the needs of production and breeding, which limits its development. On the one hand, these caught wild Portunus trituberculatus may carry infectious viruses, resulting in a very low survival rate (5-10%), causing great losses to the development of industry and regional economy; on the other hand, growth rate is an important economic factor. traits, the economic value of large-scale Portunus trituberculatus is obvio...

AI Technical Summary

Problems solved by technology

Traditional group breeding cannot take into account the two traits of growth rate and survival rate. Since swimming crabs do not have suitable individual markers, they cannot be selected by evaluating individual breeding values.

According to the characteristics of the species and the current research progress, the breeding method of the family is better, but because the individual markers have not been resolved, the family cannot be mixed, and must be cultured separately in different grids or ponds. During the breeding process, the density is one. The dynamic change value and water quality factors are not the same, so it is impossible to accurately evaluate the growth traits; if the traditional molecular marker-assisted breeding method is used, the family polyculture is first carried out, and then the molecular markers are used for individual identification, so that the family can be accurately evaluated. Growth and survival traits, but there are the following disadvantages: a. There are many individuals, and the workload is huge; b. Individual muscle or other tissues need to be extracted for molecular marker individual screening experiments. After the crab body is damaged, it is easy to be infected and die, resulting in difficulty in passage