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Application of gliocladium roseum NF-06 and conidia in inhibiting fusarium graminearum perithecium

A technology of Glioma pink and NF-06, applied in the field of microorganisms, can solve problems such as unsatisfactory chemical control and toxin reduction effects, affecting wheat quality and agricultural ecological environment safety, resistance to Fusarium graminearum, etc., to achieve green Effects of Controlling Wheat Scab

Active Publication Date: 2021-05-04
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional wheat scab control strategy is mainly based on chemical control, which is mainly carried out at the heading and flowering stage of wheat. What is important is that the continuous use of chemical pesticides not only causes the resistance of Fusarium graminearum, but also seriously affects the quality of wheat and the safety of the agricultural ecological environment, which puts great pressure on wheat production

Method used

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  • Application of gliocladium roseum NF-06 and conidia in inhibiting fusarium graminearum perithecium
  • Application of gliocladium roseum NF-06 and conidia in inhibiting fusarium graminearum perithecium
  • Application of gliocladium roseum NF-06 and conidia in inhibiting fusarium graminearum perithecium

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preparation example Construction

[0023] The invention provides a method for preparing conidia of Glioplasma pink NF-06, comprising the steps of:

[0024] (1) Insert Glioma pink NF-06 into the spore-forming medium, and cultivate to obtain a culture solution containing conidia;

[0025] (2) Filtrate the culture solution containing conidia, centrifuge the filtrate, and take the precipitate to obtain NF-06 conidia.

[0026] In the present invention, the classification of the NF-06 is named Glioma pink, and the Latin name is Clonostachysrosea. The depository unit is the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee, and the address is Beichen West, Chaoyang District, Beijing. No. 3, Courtyard No. 1, the preservation date is August 07, 2018, and the preservation number is CGMCCNo.16262.

[0027] In the present invention, the sporulation medium is CMC liquid medium.

[0028] In the present invention, the CMC liquid medium includes the following components ...

Embodiment 1

[0038] In an incubator at 25°C, after Fusarium graminearum overgrows on the carrot culture medium with a diameter of 6cm plastic plate, add sterile 0.1% Tween-20 (Tween-20), and use a sterilized spoon to strain the aerial The hyphae are overwhelmed to make them attach to the surface of the medium, and the results are shown in figure 1 .

[0039] Insert the isolated Gliocladium rosea NF-06 into the CMC sporulation medium, cultivate it for 2 days, filter the filtrate with a sterilized filter cloth, and centrifuge the filtrate at 3000rpm for 4min, and the precipitate is Glioma rosea NF-06. -06 conidia. Prepare the conidia of Glioplasma pink NF-06 with sterile water at a concentration of 1×10 5 cfu / mL of the suspension.

[0040] 10 μL of the obtained NF-06 conidia suspension was sprayed on the culture medium of Fusarium graminearum plate as a treatment group, and those without spraying the NF-06 conidia suspension were used as a control group, with 3 replicates in each group. ...

Embodiment 2

[0047] According to the method of Example 1, the Gliocladium pinkica NF-06 that is isolated is formulated into a concentration of conidia that is 1×10 6 cfu / mL of the suspension.

[0048] Referring to CN110741889A, a method for predicting the richness of the initial infection source of wheat scab in Example 1, preparing corn stalks and selecting the pathogen Fusarium graminearum as the inoculum , prepare a spore suspension of the inoculum strain, and inoculate corn stover. The straw inoculated with Fusarium graminearum was grown in a 25°C incubator for 24 hours, and the NF-06 conidia suspension was mixed with 1×10 6 cfu / mL was sprayed on the corn stalks inoculated with Fusarium graminearum as the treatment group, and the stalks not sprayed with NF-06 conidia suspension were used as the control group, with 3 replicates in each group. The stalks of the treatment group and the control group were placed in the incubator at 25°C for 6-7 days. After the cultivation, the straw was...

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Abstract

The invention relates to the technical field of microorganisms, in particular to application of gliocladium roseum NF-06 and conidia in inhibiting fusarium graminearum perithecium. Under the large background that crop straw is fully returned to the field, the prepared gliocladium roseum NF-06 conidia are sprayed to inhibit generation of the fusarium graminearum perithecium, so that primary infection sources of wheat scab are reduced, and the green control purpose of forward control and comprehensive control of the wheat scab is achieved. The fusarium graminearum perithecium inhibition rate is high and is 80-90%, and the gliocladium roseum NF-06 and conidia are safe and free of pollution.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to the application of Gliocladium pinkica NF-06 and conidia in inhibiting Fusarium graminearum asthecia. Background technique [0002] The pathogen of wheat head blight is mainly Fusarium graminearum, which is a filamentous ascomycete fungus. According to its growth and development, it can be divided into two stages: asexual stage and sexual stage. The spores produced during the asexual period are called conidia; the spores produced during the sexual period are called ascospores. Studies have shown that Fusarium graminearum overwinters in the form of saprophytic mycelia on diseased plant residues such as corn, wheat, and rice. The warm and humid weather in spring is conducive to the production and maturation of astheci of Fusarium graminearum. During the flowering stage of wheat, the ascus in the perithecia that matures on the surface of the plant residue will forcefully eject a l...

Claims

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Application Information

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IPC IPC(8): C12N3/00A01N63/30A01P3/00C12R1/645
CPCC12N3/00A01N63/30
Inventor 孙润红杨丽荣陈岩岩夏明聪张洁徐文武超
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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