Pleione bulbocodioides extract and application thereof in inhibiting plant pathogenic fungi

A technology of phytopathogenic fungus, solitary orchid, applied in the direction of chemicals, applications, and plant growth regulators for biological control, which can solve the problems of accelerated mutation of pathogenic fungi and weakened efficacy of chemical fungicides, etc., to achieve inhibition Excellent activity, significant inhibitory activity, abundant resources

Active Publication Date: 2021-03-16
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Due to the long-term use or abuse of chemical pesticides, pathogenic bacteria have developed severe resistance to many chemical agents; at the same time, the mutation speed of pathogenic fungi has accelerated, which has weakened the efficacy of traditional chemical fungicides, and the concentration of pesticides used in agricultural production is also increasing. large, leading to increasingly prominent environmental problems

Method used

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  • Pleione bulbocodioides extract and application thereof in inhibiting plant pathogenic fungi
  • Pleione bulbocodioides extract and application thereof in inhibiting plant pathogenic fungi
  • Pleione bulbocodioides extract and application thereof in inhibiting plant pathogenic fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take the pseudobulb of Suanlan orchid L., put it in an oven at 55°C and dry it, then crush it with a pulverizer and pass it through a 20-mesh sieve to collect the powder for later use; weigh 1000g of the powder, add 8000 mL of ethanol solution with a volume concentration of 70%, Heat to boiling and reflux for extraction for 2 hours, collect the filter residue and extract a by filtration; add 6000 mL of ethanol solution with a volume concentration of 70% to the filter residue, boil again, reflux for extraction for 2 hours, and filter to obtain extract b; combine the two extractions solution, concentrated under reduced pressure to obtain 320g of the pseudobulb extract of Suanlan daflora; the obtained pseudobulb extract of Suanlan dahuahua was mixed with 600g D101 macroporous adsorption resin; the mixed sample was packed in a column by wet method Purify on D101 macroporous adsorption resin chromatographic column, the volume of this chromatographic column is 12.0 L; When elu...

Embodiment 2

[0037] Take the pseudobulb of Suanlan orchid L., put it in an oven at 55°C and dry it, then crush it with a pulverizer and pass it through a 20-mesh sieve to collect the powder for later use; weigh 1000g of the powder, add 8000 mL of ethanol solution with a volume concentration of 75%, Heat to boiling and reflux for extraction for 2 hours, collect the filter residue and extract a by filtration; add 6000 mL of ethanol solution with a volume concentration of 75% to the filter residue, boil again, reflux for extraction for 2 hours, and filter to obtain extract b; combine the two extractions solution, concentrated under reduced pressure to obtain 311g of Pseudobulb extract of Suanlan daflora; the obtained Pseudobulb extract of Suanlan daflora was mixed with 600g D101 macroporous adsorption resin; the mixed sample was packed in a wet column in Purify on D101 macroporous adsorption resin chromatographic column, the volume of this chromatographic column is 12.0 L; When eluting, use 36...

Embodiment 3

[0040] Take the pseudobulb of Suanlan orchid L., put it in an oven at 55°C and dry it, then crush it with a pulverizer and pass it through a 20-mesh sieve to collect the powder for later use; weigh 1000g of the powder, add 8000 mL of ethanol solution with a volume concentration of 65%, Heat to boiling and reflux for extraction for 2 hours, collect the filter residue and extract a by filtration; add 6000 mL of ethanol solution with a volume concentration of 65% to the filter residue, boil again, reflux and extract for 2 hours, filter to obtain extract b; combine the two extractions solution, concentrated under reduced pressure to obtain 336g of the pseudobulb extract of Suanlan daflora; the mixed sample of the obtained pseudobulb extract of Suanlan Dahua with 600g D101 macroporous adsorption resin; the mixed sample was packed in a column by wet method Purify on D101 macroporous adsorption resin chromatographic column, the volume of this chromatographic column is 12.0 L; when elu...

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Abstract

The invention discloses a preparation method and application of a pleione bulbocodioides extract capable of efficiently inhibiting plant pathogenic fungi, and the method comprises the following steps:drying and pulverizing pleione bulbocodioides pseudobulbs, carrying out reflux extraction by using an aqueous ethanol solution, carrying out macroporous adsorption resin column chromatography purification, and performing concentrating under reduced pressure to dryness to obtain the pleione bulbocodioides extract. The preparation method is simple and easy to operate, the total yield is 10.5%-13.2%, the preparation method is suitable for large-scale production, and the obtained extract has remarkable inhibitory activity on plant pathogenic fungi and has good application prospects. The activityof the compound in inhibiting cabbage anthracnose pathogenic bacteria, guava anthracnose pathogenic bacteria and gliocladium tenuissimum is superior to that of an environment-friendly botanical pesticide ethylicin which is used in quantity at present.

Description

technical field [0001] The invention relates to a preparation method for extracting an extract having the activity of inhibiting plant pathogenic fungi from a plant body - the pseudobulb of the orchid orchid. Background technique [0002] Due to the long-term use or abuse of chemical pesticides, pathogenic bacteria have developed serious resistance to many chemical agents; at the same time, the mutation speed of pathogenic fungi has accelerated, which has weakened the efficacy of traditional chemical fungicides, and the concentration of pesticides used in agricultural production is also increasing. large, leading to increasingly prominent environmental problems. Many studies have shown that in the long-term struggle with phytopathogenic fungi, plants themselves can produce secondary metabolites that inhibit phytopathogenic fungi, such as acetoicin, matrine, camptothecin, eugenol, etc. Inhibit plant pathogenic fungi, and not easy to produce resistance. Therefore, finding na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/40A01P3/00
CPCA01N65/00A01N65/40
Inventor 吴沙沙倪林徐会有翟俊文彭东辉李威周育真艾叶裴新颖
Owner FUJIAN AGRI & FORESTRY UNIV
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