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Method for control of tobacco nematode diseases with composite nematicidal microorganism

A microbial control and nematode killing technology, which is applied in the field of tobacco nematode disease prevention and control, can solve the problems of unsatisfactory control effects of biocontrol agents, and achieve the effect of improving the ability of nematode resistance, high control rate and outstanding control effect

Inactive Publication Date: 2020-02-11
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: the problem that the control effect of the existing field crop nematode disease biocontrol agents is not ideal

Method used

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  • Method for control of tobacco nematode diseases with composite nematicidal microorganism
  • Method for control of tobacco nematode diseases with composite nematicidal microorganism
  • Method for control of tobacco nematode diseases with composite nematicidal microorganism

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specific Embodiment 1

[0037] Organize the tobacco field soil according to the routine requirements. Select robust, tidy tobacco seedlings with a plant height of about 10 cm, with a plant spacing of 50 cm and a nest depth of 15 cm. In each hole for tobacco seedling cultivation, 20ml of mixed bacterial solution was first applied, and then the tobacco was managed according to the tobacco seedling cultivation norms such as covering soil and watering. It can be seen that the tobacco seedlings grow normally. After the tobacco plant grows, the effect of nematode control can be seen.

specific Embodiment 2

[0039] Select tomato seedlings that grow normally, and dig small holes beside the roots, deep to the side roots. In each hole, apply 50ml of mixed bacterial solution and then cover with soil, and then manage according to the normal management method of vegetable seedlings. It can be seen that the vegetable seedlings grow normally.

specific Embodiment 3

[0041]Organize the tobacco field soil according to the routine requirements. Select robust, tidy tobacco seedlings with a plant height of about 10 cm, with a plant spacing of 50 cm and a nest depth of 15 cm. In each hole for tobacco seedling cultivation, 100ml of mixed bacterial solution was first applied, and then the tobacco was managed according to the tobacco seedling cultivation norms such as covering soil and watering. It can be seen that the tobacco seedlings grow normally. After the tobacco plant grows, the effect of nematode control can be seen.

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Abstract

The invention relates to a method for control of tobacco nematode diseases with a composite nematicidal microorganism. The composite nematicidal microorganism is composed of the following four microorganisms: Gliocladium roseum, Paecilomyces lilacinus (Thom.) Samson, Burkholderia arboris and Sarratia entomophilus. Specifically, the Burkholderia arboris is Burkholderia arboris J211, and the preservation number is CCTCC NO:M 2019747. The Burkholderia arboris is a strain obtained by new research, and research finds that the strain has a high control effect on nematodes, and new functions are in continuous research; (2) on the basis of nematode control with the Burkholderia arboris, the other three strains are mixed, and the related volume ratio therein is controlled, so that the results of outstanding control effect and high control rate (shown as the embodiment) are realized; and 3) the composite nematicidal microorganism adopted by the method provided by the invention has obvious capability of improving the nematode disease resistance of field crops, and at the same time can improve commodity quality and yield of crops.

Description

technical field [0001] The invention belongs to the technical field of biological control; in particular, it relates to the technical field of methods for controlling tobacco nematode diseases by using compound nematicidal microorganisms. Background technique [0002] Nematodes are a very common plant pest during the growth of crops. Some plant parasitic nematodes can damage the root tissue of plants, resulting in stunted root development and nutrient absorption. Some nematodes also spread other diseases. Nematodes are estimated to cost crops the equivalent of $100 billion a year. [0003] Common nematode control methods mainly include fumigation and the use of chemical pesticides to kill insects. The greener method is to use microbial agents with nematode function. Fumigation has a huge destructive effect on the environment, and the country has explicitly banned its use. Chemically synthesized pesticides have various hazards because of their residues, harmfulness to the...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01N63/36A01N63/20A01P5/00
Inventor 陈穗云王兴红张仁军
Owner YUNNAN UNIV
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