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Seedling breeding and rapid propagation cultivation method for traditional Chinese herbal medicine anoectochilus formosanus

A cultivation method and a technology of traditional Chinese medicinal materials, which are applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of A. strains, and the effect of improving the planting ability

Inactive Publication Date: 2013-07-17
赖新权
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Problems solved by technology

[0004] The seeds of clematis do not have endosperm and have no germination ability. Only under the symbiosis of fungi can the germination of seeds be promoted, but the germination rate is low. Propagation by split root method or cutting method takes a long time and the multiplication factor is not high

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  • Seedling breeding and rapid propagation cultivation method for traditional Chinese herbal medicine anoectochilus formosanus
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  • Seedling breeding and rapid propagation cultivation method for traditional Chinese herbal medicine anoectochilus formosanus

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Embodiment Construction

[0012] The method of seedling selection and rapid propagation of the Chinese herbal medicine clematis clematis is to collect wild clematis plants, rinse the roots with sterile water, cut into 0.5-1cm root segments, and sterilize the surface with 0.1% HgCl2 for 6-8min. Then rinse it with sterile water, then sterilize the surface with 75% ethanol for 3-5 seconds, then rinse it with sterile water, inoculate the washed root section on a PDA plate, and cultivate it at a constant temperature of 25°C. After the fungal hyphae grow in the segment, the tip mycelium is picked for purification and culture; to obtain the purified mycorrhizal fungus Gliocladium genus AR-11 or AR-13 orchid mushroom as a symbiotic fungus of Aurantia chinensis, to realize industrial production of golden clematis Clematis symbiotic seedlings.

[0013] MS medium is selected as the inoculation medium of A. clematis, and 6-BA cytokinin and NAA plant growth regulator are also added in the MS medium to promote the r...

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Abstract

The invention discloses a seedling breeding and rapid propagation cultivation method for traditional Chinese herbal medicine anoectochilus formosanus. The seedling breeding and rapid propagation method includes collecting wild anoectochilus formosanus seedlings, cutting roots into small pieces prior to sterilizing and washing, cultivating the small pieces of the wild anoectochilus formosanus plants at the constant temperature of 25 DEG C to obtain purified mycorrhiza fungi gliocladium AR-11 or AR-13 mycena anoectochila to be served as symbiotic fungus of the anoectochilus formosanus, and finally realizing industrialization production of anoectochilus formosanus symbiotic seedlings. The MS culture medium is selected to be a seedling breeding medium, 6-BA cytokinin and NAA plant growth regulator are further added into the MS culture medium, a cultivation field to plant the anoectochilus formosanus is selected and managed scientifically; by the method, disease resistance and survival rate of tissue culture seeding of the anoectochilus formosanus can be improved, so that breeding speed is fast, and industrialized seedling production and mass production of the anoectochilus formosanus can be realized.

Description

technical field [0001] The invention relates to plant selection and cultivation techniques, in particular to a traditional Chinese medicine [0002] A method for selection and rapid propagation of clematis aureus seedlings. Background technique [0003] There are at least 3 species of clematis produced now, namely A. formosanus, A. formosanus and A. koshunensis. The quality of clematis is mainly determined by the content of its active ingredients. By comparing the contents of different medicinal components of clematis, a single plant of high-quality material is selected, and an excellent single plant is propagated through explant cloning technology; it can also be induced by polyploidy, New strains that are suitable for cultivation and have high content of active ingredients are selected by hybridization with local native species, screening and breeding. [0004] The seeds of clematis do not have endosperm and have no ability to germinate. The germination of the seeds can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 赖新权
Owner 赖新权
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