40 results about "Anoectochilus setaceus" patented technology

The invention provides a water culture technique for anoectochilus formosanus and relates to a pollution-free water (fog) culture production technique for Chinese medicament anoectochilus formosanus. The water culture technique adopts plastic disks and water grooves as culture containers and comprises the following steps of: transplanting the seedlings which are prepared by tissue culture and subjected to cleaning and sterilizing into the plastic disks; wherein the plastic disks are placed in the water grooves with nutrient solution; and performing culture management by fertilizing, changing the nutrient solution, atomizing, lighting, controlling humidity, controlling temperature and changing air. The water culture technique has the advantages that: the survival rate of transplanting the noectochilus formosanus seedlings prepared by tissue culture can be improved to over 98 percent; the fresh weight of the finished products in each square meter area is over 800 g; the production process is simple; the yield is high; the occupied area is small; and the water culture technique is suitable for culture by intensive and pollution-free facilities or scattered culture by farmers.

The invention belongs to the field of a traditional Chinese medicinal material, and particularly relates to a method for carrying out tissue cultivation propagation of anoectochilus roxburghii by an intermittent submerged bioreactor. The method comprises the steps of early-stage preparation, explant selection, and aseptic treatment, inoculation, cultivation of the bioreactor, and the like. Multiplication and rooting and strong seedling cultivation are finished one time in the reactor; the method has the characteristics of being short in period, high in tissue culture seedling quality and the like; and a method with low cost and high efficiency is provided for rapid propagation of tissue cultivation of the anoectochilus roxburghii.

The invention provides a method for planting Anoectochilus formosanus. The method is characterized by preparing a planting matrix by mixing peat, humus soil, carbonized rice hull and river sand in a ratio of 1:1, and comprising the following steps of: (A), exposing matrix soil to the sun for 4 to 6 hours, spraying a thiophanate solution diluted by water in an amount which is 800 times the volume of thiophanate or a carbendazim solution diluted by water in an amount which is 600 to 1,000 times the volume of carbendazim, paving on a planting seedling bed, and controlling the thickness to be 15 to 20cm; (B), selecting strong Anoectochilus formosanus seedlings which have the plant height of 2cm and have more than two roots and branches, and planting in the matrix according to the plant row distance of 5*10-15cm; and (C), watering, spraying a nutrient solution and applying a compound fertilizer or farmyard manure according to the circumstances after plantation, spraying a bactericide or a pesticide, and harvesting after 5 to 7 months. The matrix is easy in material selection, low in cost and high in compactness, has a long using period and a good water and fertilizer retention effect, and is applicable to the healthy growth of the Anoectochilus formosanus, the survival rate is high, the labor intensity is low, and good social and economic benefits can be achieved.

The invention discloses a technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.)Lindl in Fujian. The technology comprises the steps of obtaining a material in the field, disinfecting the material, performing induction culture on adventitious buds, performing one-time grown-up seedling culture, transplanting bottle seedlings, performing underwood planting, and preparing a finished product. The technology solves the problems that the seeds of anoectochilus roxburghii (Wall.) Lindl are tiny, embryos are not completely developed, and the seeds are difficult to germinate under a natural condition; the method also solves the problems that in the prior art, a medicinal herb resource of the anoectochilus roxburghii (Wall.) Lindl is rare, and especially, the components for strongly easing pain, diminishing inflammation and resisting cancer contained in plants can not meet clinical requirements and daily requirements for enhancing human body immunity and the like. According to the technology, the wild anoectochilus roxburghii (Wall.) Lindl is cultured by a one-time grown-up seedling method and is subjected to field return-to-nature ex-situ plantation, so that the survival rate of the anoectochilus roxburghii (Wall.) Lindl is high, the yield is improved by 1-2 times, and the plants contain sufficient substances and are good in condition and quality.

The invention relates to an auxiliary material and cultivation method for realizing field growth of anoectochilus formosanus. According to the auxiliary material, the weight mixing ratio of soddy soil is more than 99.5 percent, and the ratio of lime is less than 0.5 percent. A spinney area close to water flow and waterfall with the canopy density of more than 78 percent is selected as a cultivation area. The method comprises the following steps: mixing the auxiliary material with the thickness of 7cm and deeply ploughed surface soil, thoroughly watering and retting for 30 days, washing the seedlings without sterilization, planting according to a spacing between rows of 3*5cm, spraying bactericides in a mist shape and watering rooting water, spraying the bactericides every 10 days in the earlier 30 days, spraying a solution of biofertilizers and monopotassium phosphate outside the roots every 15 days after 30 days for 3-5 times, utilizing clean spring water for cultivation, covering a shading net on the spinney area in fine days and covering a film in rainy days on the spinney area, wherein two sides of the net and the film need to be 20cm away from the ground, and two ports are not shaded. The survival rate is 90 percent. The investment on infrastructures is avoided, natural resource bed soil cultivation is utilized, soil fertility, water, bacteria, ventilation and illumination are regulated, return-to-nature growth of anoectochilus formosanus is realized, the auxiliary material is low in investment, slight in pollution and high in yield and suitable for large-scale cultivation, and the fresh product is similar to a wild plant.

The invention discloses a seedling breeding and rapid propagation cultivation method for traditional Chinese herbal medicine anoectochilus formosanus. The seedling breeding and rapid propagation method includes collecting wild anoectochilus formosanus seedlings, cutting roots into small pieces prior to sterilizing and washing, cultivating the small pieces of the wild anoectochilus formosanus plants at the constant temperature of 25 DEG C to obtain purified mycorrhiza fungi gliocladium AR-11 or AR-13 mycena anoectochila to be served as symbiotic fungus of the anoectochilus formosanus, and finally realizing industrialization production of anoectochilus formosanus symbiotic seedlings. The MS culture medium is selected to be a seedling breeding medium, 6-BA cytokinin and NAA plant growth regulator are further added into the MS culture medium, a cultivation field to plant the anoectochilus formosanus is selected and managed scientifically; by the method, disease resistance and survival rate of tissue culture seeding of the anoectochilus formosanus can be improved, so that breeding speed is fast, and industrialized seedling production and mass production of the anoectochilus formosanus can be realized.

The invention belongs to the technical field of biological medicines, and discloses a plant-derived anti-aging composition and application thereof. The composition contains NMN and a plant extract, wherein the plant extract is any one or more of a rosemary extract, a cyanotis arachnoidea extract, an anoectochilus roxburghii extract and an emblic leafflower fruit extract. According to the plant-derived anti-aging composition disclosed by the invention, the advantages of traditional Chinese medicines / medicinal and edible plants in China are fully exerted, an obvious synergistic effect can be exerted on the premise that a large amount of natural plant extracts are used and the use amount of NMN is greatly reduced, and an excellent anti-aging effect of a product is ensured; in addition, various side effects such as inflammatory response and liver injury caused by the NMN can be greatly reduced, the product stability is very good, and very good application value is achieved in the aspect ofdeveloping alternative anti-aging products of existing NMN products.

The invention discloses a composition for promoting wound healing, and a preparation method thereof, and concretely belongs-the field of traditional Chinese medicinal compositions. The composition comprises, by weight, 20-30 parts of Hemsley Rockvine Root, 10-20 parts of Climbing Groundsel Herb, 5-10 parts of Isatis Root, 20-30 parts of Anoectochilus roxburghii, 10-15 parts of Euphorbia fischeriana Steud, 5-10 parts of chitosan, 3-7 parts of Weeping Forsythia, 10-18 parts of honeysuckle flowers, 5-10 parts of Setaria viridis, 5-10 parts of Herba Plantaginis, 2-8 parts of mint, 3-7 of tea leaves, 5-10 parts of Semen Momordicae, 8-16 of rose flowers, 3-7 parts of lemons and 8-15 parts of honey. The composition has the advantage of promotion of wound healing and prevention of wounds from being infected by pathogens.

A dendrobium candidum health beverage is prepared from raw material comprising component A and component B, wherein the component A comprises ingredients by weight part: 45-60 parts of dendrobium candidum, 3-7 parts of penthorum chinense pursh, 10-15 parts of gynostemma pentaphyllum, 5-8 parts of puerarin, 3-8 parts of radix glycyrrhizae, 6-8 parts of adenophora stricta, 4-8 parts of shaddock peels and 3-5 parts of anoectochilus roxburghii, and the component B comprises ingredients by weight part: 40-45 parts of milk powder, 5-10 parts of demineralized whey powder, 0.4-0.8 part of sodium carboxymethyl cellulose, 0.03-0.1 part of nisin, 2-3 parts of sodium alginate and 0.05-0.14 part of magnesium stearate. The dendrobium candidum health beverage uses the dendrobium candidum as main raw material, adds the milk powder and a small quantity of additives in the dendrobium candidum, and contains various nutrient elements. The dendrobium candidum health beverage is suitable for white-collar urban people in sub-health status, and particularly suitable for long-term alcohol drinking social engagement people, and particularly has good maintenance effect for human livers.

The invention belongs to the field of molecular biology and particularly relates to an anoectochilus roxburghii DNA extracting method suitable for RAPD analysis. By the method, high-purity anoectochilus roxburghii DNA can be obtained at high yield according to the plant features of anoectochilus roxburghii. The method has the advantages that in traditional CTAB extract, PVP use amount is increased to 4%, and salinity is increased to 2.0mol / L; chloroform and isoamyl alcohol with the volume ratio of 24:1 are used; after isopropanol precipitates DNA, 500 microliter of high salt TE (100mmol / L Tris-HC1, pH8.0; 20mmol / L EDTA, pH8.0; 1.4mol / L NaCl) is added for reprecipitation; RNaseA is added finally. In subsequent RAPD experiments, the anoectochilus roxburghii DNA extracted by the method is clear in band, bright, stable and high in repeatability.

The invention discloses a method for cultivating wild anoectochilus formosanus, which comprises site selection, base construction, seedling cultivation, seedling treatment, field management and other steps. A standard planting mode is formed for the wild anoectochilus formosanus by automatic greenhouse management, breeding tissue culture seedling for sound seedling, seedling coating and diseases and insects prevention before transplantation, and rational and timely field management in the later period. The wild anoectochilus formosanus cultivated in the planting mode is high in survival rate, and is not easily influenced by severe field environment and diseases and insects, so that an important basis is provided for the large-scale and industrial plantation of the wild anoectochilus formosanus.

The invention relates to the technical field of plant cultivation, in particular to a cultivation and propagation method of Anoectochilus roxburghii, comprising the steps of S1, selecting an environment, to be specific, selecting a hilly land with an elevation of 300-1200 m and with gullies having forest and water at a suitable temperature of 20-28 DEG C, thereby ensuring shadiness, enough water for irrigation, wind avoiding and warmth retention in winter, less heat dissipation, constant humidity, and good traffic convenience; S2, setting up a greenhouse having height of 1.2-1.5 m, width of 6m, waist height of 1.5-1.8 m and length of 20-30 m, setting up bamboo or steel-framed seedbeds with width of about 1.2-1.5 m and height of about 80 cm within the greenhouse, providing aisles about 1 min width between the seedbeds, covering the top of the greenhouse with a layer of plastic film, and covering the plastic film with two layers of sun-shading nets and a layer of insect-proof net; S3,performing acclimation cultivation before field planting of tissue culture seedlings; S4, cultivating and propagating Anoectochilus roxburghii, to be specific, carrying out cultivation and propagation, controlling cultivation and propagation density, and determining cultivation and cultivation depth. The greenhouse is designed strictly according to the growth characteristics of Anoectochilus roxburghii; excellent growth conditions are provided to ensure the growth speed, survival rate and yield of Anoectochilus roxburghii.

The invention discloses penicillium oxalicum HY181-2, a preparation method and application thereof. The penicillium oxalicum HY181-2 is preserved in Guangdong Microbial Culture Collection Center, andthe preservation number is GDMCC No: 61142. The penicillium oxalicum HY181-2 has the effect of preventing and treating stem rot and / or anthracnose of anoectochilus formosanus.

The invention discloses a method for regenerating and planting medicinal anoectochilus roxburghii with the stem removed but the root left, which has the key point of regeneration with the root left and stem removed. First, strong tissue culture seedling are elected and then transmitted to be planted in a mixed substrate consisting of soil, coarse sand and wood dust after plantlet hardening and watered with a mixed liquid of root agent and carbendazim wettable powder. Field management, fertilization and disease preventing and treating are required to be respected during the growth period of the anoectochilus roxburghii. By applying the method of regeneration with the stem removed and the stem left, the anoectochilus roxburghii stems on the ground are harvested but a stem node at each stem is left before the bolting and blossom of the anoectochilus roxburghii. After harvesting, the remained plant is sprayed with a mixed liquid of 6-benzyl aminopurine and carbendazim wettable powder. Themethod aims at solving the problem of narrow availability of the anoectochilus roxburghii seedling and easily sprouting of the stem node thereof and adopts the production method of regeneration with the stem removed and the stem left, thereby improving the seedling use ratio, guaranteeing the product supply and realizing continuous production. Meanwhile, the complete retaining of the root system creates the good condition for the quick growth of new sprouts and therefore, the regeneration method of the invention has comparatively high production efficiency and good economic benefit.

The invention discloses an oral kidney-tonifying and arthralgia-eliminating traditional Chinese medicine composition for treating ankylosing spondylitis and a preparation method of the oral kidney-tonifying and arthralgia-eliminating traditional Chinese medicine composition, and belongs to the technical field of traditional Chinese medicines. The invention discloses a traditional Chinese medicine composition. Comprising the following raw materials in parts by weight: 12-16 parts of tripterygium wilfordii, 20-40 parts of herba epimedii, 8-10 parts of orange peel, 10-12 parts of semen cassiae, 8-10 parts of poria cocos, 6-8 parts of lotus leaves, 1-3 parts of Fritillaria yunnanensis extract, 2-4 parts of Anoectochilus roxburghii extract, 2-4 parts of Ailanthus tonkinensis extract, 2-4 parts of dandelion, 2-5 parts of semen plantaginis, 1-2 parts of red ginseng, 2-4 parts of radix scutellariae, 2-4 parts of bighead atractylodes rhizome, 4-6 parts of gypsum, 1-3 parts of liquorice, 2-3 parts of fig root, 1-3 parts of herba aristolochiae, 1-2 parts of milk thistle, 2-4 , 2 parts of radix puerariae and 1-2 parts of saffron crocus. The traditional Chinese medicine composition disclosed by the invention can be used for effectively treating the ankylosing spondylitis.

The invention discloses evodia fruit compound essential oil with effects of alleviating pain and resisting tumors and a preparation method thereof. The essential oil is prepared from the following rawmaterials in parts by weight: 45-55 parts of evodia fruits, 5-10 parts of Chinese fan palm seeds, 3-8 parts of poria cocos, 30-40 parts of rhizoma arisaematis, 20-30 parts of anoectochilus roxburghii, 10-15 parts of turmeric root-tuber, 10-15 parts of ligusticum wallichii, 10-15 parts of turmeric and 5-10 parts of hemsley rockvine roots. The evodia fruit compound essential oil has high cell permeability, drug resistance prevention and good pain-alleviating and tumor-resisting effects and can be used for enhancing the immune modulating function due to the good synergistic effect among the ingredients.

The invention provides an organic cultivation method for anoectochilus formosanus in a sealed environment, and aims at solving the problems that in the prior art, the yield of anoectochilus formosanus is low, and management is troublesome. The invention discloses a method for organic cultivation of anoectochilus formosanus in a sealed environment. The method comprises the following steps: preparation of a cultivation medium: uniformly mixing and stirring peat, vermiculite and perlite to obtain a mixed material A; sterilizing the mixed material A; adding a probiotic liquid into sterile water, uniformly stirring to obtain a mixed diluent, spraying the mixed diluent on the mixed material A, and performing anaerobic fermentation to obtain a culture medium after the fermentation is finished; the culture medium is laid at the bottom of the transparent plastic sealing bag, the anoectochilus formosanus is planted in the transparent plastic sealing bag, and an opening in the upper end of the transparent plastic sealing bag is sealed. The transparent plastic sealing bag, the culture medium and the probiotics are matched for use, a synergistic effect is achieved, and the single-plant size and mass of anoectochilus formosanus are obviously higher than those of anoectochilus formosanus cultivated in an existing cultivation mode.

The invention relates to the technical field of plant cultivation, and discloses a cultivation method of sunshine seedlings, a culture medium is utilized to culture plants, the culture medium is a transparent tank type glass bottle, and the culture medium contains an MS basic culture medium, a basic additive, garlic oil, a scutellaria baicalensis extract, onions and crab shell powder. The basic additive comprises naphthylacetic acid, 6-benzyladenine, activated carbon, cane sugar and agar, and on the basis of the scheme, blending is performed by using the following method: S1, disinfecting a culture container, disinfecting a culture medium, a bottle cap and a plastic tray for inoculation, and storing for later use. By adding the garlic oil, the radix scutellariae extract, the onions and the crab powder into the MS basic culture medium, the sterilization and parasite expelling effects can be achieved for culture of the anoectochilus formosanus sunshine seedlings, so that the survival rate of the anoectochilus formosanus sunshine seedlings is increased, and residues of chemical sterilization agents are avoided.

The invention relates to the technical field of plant tissue culture, in particular to a liquid tissue culture device for anoectochilus formosanus sprouts. The device comprises a tissue culture bottle, wherein a first culture bottle body is arranged in the tissue culture bottle; one or more second culture bottle bodies are embedded in the first culture bottle body, and are hollow and bottomless; the bottom ends of the second culture bottle bodies are connected with the bottom end of the first culture bottle body, and the joint parts of the first culture bottle body and the second culture bottle bodies are bottomless. The first culture bottle body and the second culture bottle bodies are arranged, so that the device has the advantages that one tissue culture bottle can be filled with two independent liquid media, quantities of the liquid media in the first and second culture bottle bodies can be respectively controlled; and when the liquid media in the second culture bottle bodies are almost consumed, the effect of drought stress is achieved, and the anoectochilus formosanus sprouts are prompted to root and root out of the second culture bottle bodies to be in contact with the liquid media in the first culture bottle body.

A production process of clematis tea; it is characterized in that: the steps of the production method are as follows: 1. Dry the clematis after picking and cleaning, and then carry out wilting in a cool place to ensure the water content of the clematis after thickening Eighty to eighty-five; 2. Cutting: cut off the air-dried clematis, each section is 1CM to 2CM, 3. Mix the thickened Tieguanyin tea with the cut clematis and knead it. Finally, fermentation is carried out. The present invention mixes the characteristics of clematis and Tiehongyin to make tea, which solves the shortcoming that clematis is too sweet and greasy, and also has the taste of Tieguanyin black tea.

The invention discloses a solid beverage capable of alleviating hangover and protecting liver and stomach and a preparation method thereof. The solid beverage is prepared from the raw materials in parts by weight of 3-5 parts of radix puerariae, 1-2 parts of fruits, 1-2 parts of anoectochilus formosanus and 1-2 parts of chrysanthemum, and the auxiliary materials comprises 1-2 parts of rice products. The solid beverage is safe, green, healthy and free of side effects; and by integrating the effects and synergistic effects of radix puerariae, fruits, anoectochilus formosanus, chrysanthemum, rice products and the like, the solid beverage has the effects of protecting liver and stomach, nourishing qi and blood, clearing away lung-heat and relieving sore throat, moistening intestines and relaxing bowel and the like; on one hand, the solid beverage can regulate qi and blood, nourish life and prolong life; and on the other hand, blood circulation can be promoted, nutrient metabolism is improved, toxins are rapidly discharged out of a body, and body immunity is improved.

The invention relates to the technical field of medicinal material planting, in particular to a facility three-dimensional seedling hardening planting method for anoectochilus formosanus. The method comprises the steps that screened anoectochilus formosanus tissue culture seedlings are placed in an environment-controllable facility plant factory, a culture medium is laid in planting trays, water tank bottom permeation type irrigation is adopted, then seedling hardening is carried out on the anoectochilus formosanus tissue culture seedlings in the facility plant factory with well setting in advance, and then the anoectochilus formosanus seedlings are transplanted into to the planting trays and are placed in three-dimensional cultivation frames in the facility plant factory with set environmental conditions for scientific planting. According to the seedling hardening planting method, the most suitable growth environment is provided for the anoectochilus formosanus tissue culture seedlings in different growth periods, and therefore rooting of the seedlings is promoted, accumulation of effective components in plant bodies is improved, and the yield and quality of anoectochilus formosanus are greatly improved.

The invention belongs to the technical field of health foods, and aims to provide compound granules with the function of relieving alcohol effect and protecting the liver and a preparation method thereof. The compound granules not only have significant functions of relieving alcohol effect and protecting the liver, but also have health-care functions, has a good taste and no side effect, can be taken for a long time, and is convenient to carry and take. The preparation method has the characteristics of simple process. The technical scheme is as follows: the compound granules with the functionof relieving alcohol effect and protecting the liver are prepared from the following raw materials in parts by weight: 2-10 parts of all grass of roxburgh anoectochilus, 2-10 parts of chimonanthus salicifolius, 2-10 parts of leave of combined spicebush, 2-10 parts of waxberries, 2-10 parts of kudzuvine roots, 1-5 parts of loquat leaves, 1-5 parts of corn, 0.2-1.0 part of bamboo leaf flavone, and 0.1-0.5 part of haematococcus pluvialis.

The invention belongs to the field of tumor treatment, and discloses a traditional Chinese medicine formula for treating lung cancer, which comprises the following traditional Chinese medicine components in parts by weight: 30g of anoectochilus formosanus, 20g of taxus chinensis, 30g of wheat bran, 20g of solanum lyratum, 30g of lucid ganoderma, 20g of sculellaria barbata, 20g of dindygulen peperomia herb, 20g of polygala fallax hemsl, 15g of lily, 20g of red kiwi root, 20g of cephalotaxus fortunei, 30g of bunge corydalis herb, 20g of nightshade, 10g of paris polyphylla, 20g of folium cycas, 6g of tetrastigma hemsleyanum, 30g of oldenlandia diffusa, 10g of russule, 15g of selaginella doederleinii hieron and 20g of serissa serissoides. According to the scheme, the medicines such as the anoectochilus formosanus, the taxus chinensis, the solanum lyratum, the lucid ganoderma, the sculellaria barbata, the dindygulen peperomia herb and the lily are effectively combined and support one another, so that the traditional Chinese medicine formula has the effects of strengthening the body resistance, eliminating evil, promoting blood circulation to remove blood stasis, detoxifying and removing stasis, is effectively used for treating lung cancer, and has the advantages of remarkable curative effect, small side effect, simplicity in preparation and low cost.

The invention discloses a culture medium sterilization equipment for breeding high-quality anoectochilus formosanus, the culture medium sterilization equipment structurally comprises a cabinet body, a front door and a control panel, liquid in a culture medium on the inner wall of the top of an inoculation bottle is gradually heated and evaporated, and carbohydrates are condensed into crystals, so that the content of the carbohydrates in the inoculation bottle is insufficient; a pushing block of a pushing plate in a pushing mechanism continuously impacts the bottom of the inoculation bottle, so that the inoculation bottle vibrates, liquid on the inner wall of the inoculation bottle is accelerated to flow downwards, gradual heating evaporation of liquid in the culture medium on the inner wall of the top of the inoculation bottle is reduced, the content of carbohydrates in the inoculation bottle is kept unchanged, and culture of anoectochilus formosanus seeds by the culture medium is facilitated; and due to the fact that the pushing block is low in moving speed and small in pushing force on the inoculation bottle, a swinging rod is arranged in a matching block and swings in a movable groove, the swinging speed of the matching block is increased, the pushing force of the matching block on the pushing block can be increased, the reset speed of the pushing block can be increased, and the up-down moving speed of the pushing block can be increased.

In order to solve the problems of poor plant growth vigour and a low light energy use ratio in a current anoectochilus roxburghii (Wall.) Lindl. culture process in the prior art, the invention provides an anoectochilus roxburghii (Wall.) Lindl. culture method capable of improving a drying rate. The anoectochilus roxburghii (Wall.) Lindl. culture method comprises the following steps of: manufacturing a culture medium; carrying out inoculation; carrying out culture: carrying out culture under a red and blue mixed illumination condition, wherein the culture is divided into three month, in the first month, illumination time is 7.5-8.5 h / d, in the second month, the illumination time is 9.5-10.5 h / d, and in the third month, the illumination time is 10.5-12.5 h / d. The culture method has the beneficial effects that red and blue light is adopted to replace traditional white light, the volume of a plant can be improved, and the growth vigour is better. In addition, effective time for the plant to absorb illumination in the first month is 7.5-8.5 h / d, the effective time for the plant to absorb the illumination in the second month is 9.5-10.5 h / d, the effective time for the plant to absorb theillumination in the third month is 10.5-12.5 h / d, and therefore, the illumination time is set into corresponding duration so as to be favorable for saving electricity energy.