38 results about "Anoectochilus setaceus" patented technology

The invention provides a water culture technique for anoectochilus formosanus and relates to a pollution-free water (fog) culture production technique for Chinese medicament anoectochilus formosanus. The water culture technique adopts plastic disks and water grooves as culture containers and comprises the following steps of: transplanting the seedlings which are prepared by tissue culture and subjected to cleaning and sterilizing into the plastic disks; wherein the plastic disks are placed in the water grooves with nutrient solution; and performing culture management by fertilizing, changing the nutrient solution, atomizing, lighting, controlling humidity, controlling temperature and changing air. The water culture technique has the advantages that: the survival rate of transplanting the noectochilus formosanus seedlings prepared by tissue culture can be improved to over 98 percent; the fresh weight of the finished products in each square meter area is over 800 g; the production process is simple; the yield is high; the occupied area is small; and the water culture technique is suitable for culture by intensive and pollution-free facilities or scattered culture by farmers.

The invention belongs to the field of a traditional Chinese medicinal material, and particularly relates to a method for carrying out tissue cultivation propagation of anoectochilus roxburghii by an intermittent submerged bioreactor. The method comprises the steps of early-stage preparation, explant selection, and aseptic treatment, inoculation, cultivation of the bioreactor, and the like. Multiplication and rooting and strong seedling cultivation are finished one time in the reactor; the method has the characteristics of being short in period, high in tissue culture seedling quality and the like; and a method with low cost and high efficiency is provided for rapid propagation of tissue cultivation of the anoectochilus roxburghii.

The invention discloses a technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.)Lindl in Fujian. The technology comprises the steps of obtaining a material in the field, disinfecting the material, performing induction culture on adventitious buds, performing one-time grown-up seedling culture, transplanting bottle seedlings, performing underwood planting, and preparing a finished product. The technology solves the problems that the seeds of anoectochilus roxburghii (Wall.) Lindl are tiny, embryos are not completely developed, and the seeds are difficult to germinate under a natural condition; the method also solves the problems that in the prior art, a medicinal herb resource of the anoectochilus roxburghii (Wall.) Lindl is rare, and especially, the components for strongly easing pain, diminishing inflammation and resisting cancer contained in plants can not meet clinical requirements and daily requirements for enhancing human body immunity and the like. According to the technology, the wild anoectochilus roxburghii (Wall.) Lindl is cultured by a one-time grown-up seedling method and is subjected to field return-to-nature ex-situ plantation, so that the survival rate of the anoectochilus roxburghii (Wall.) Lindl is high, the yield is improved by 1-2 times, and the plants contain sufficient substances and are good in condition and quality.

The invention relates to an auxiliary material and cultivation method for realizing field growth of anoectochilus formosanus. According to the auxiliary material, the weight mixing ratio of soddy soil is more than 99.5 percent, and the ratio of lime is less than 0.5 percent. A spinney area close to water flow and waterfall with the canopy density of more than 78 percent is selected as a cultivation area. The method comprises the following steps: mixing the auxiliary material with the thickness of 7cm and deeply ploughed surface soil, thoroughly watering and retting for 30 days, washing the seedlings without sterilization, planting according to a spacing between rows of 3*5cm, spraying bactericides in a mist shape and watering rooting water, spraying the bactericides every 10 days in the earlier 30 days, spraying a solution of biofertilizers and monopotassium phosphate outside the roots every 15 days after 30 days for 3-5 times, utilizing clean spring water for cultivation, covering a shading net on the spinney area in fine days and covering a film in rainy days on the spinney area, wherein two sides of the net and the film need to be 20cm away from the ground, and two ports are not shaded. The survival rate is 90 percent. The investment on infrastructures is avoided, natural resource bed soil cultivation is utilized, soil fertility, water, bacteria, ventilation and illumination are regulated, return-to-nature growth of anoectochilus formosanus is realized, the auxiliary material is low in investment, slight in pollution and high in yield and suitable for large-scale cultivation, and the fresh product is similar to a wild plant.

The invention discloses a seedling breeding and rapid propagation cultivation method for traditional Chinese herbal medicine anoectochilus formosanus. The seedling breeding and rapid propagation method includes collecting wild anoectochilus formosanus seedlings, cutting roots into small pieces prior to sterilizing and washing, cultivating the small pieces of the wild anoectochilus formosanus plants at the constant temperature of 25 DEG C to obtain purified mycorrhiza fungi gliocladium AR-11 or AR-13 mycena anoectochila to be served as symbiotic fungus of the anoectochilus formosanus, and finally realizing industrialization production of anoectochilus formosanus symbiotic seedlings. The MS culture medium is selected to be a seedling breeding medium, 6-BA cytokinin and NAA plant growth regulator are further added into the MS culture medium, a cultivation field to plant the anoectochilus formosanus is selected and managed scientifically; by the method, disease resistance and survival rate of tissue culture seeding of the anoectochilus formosanus can be improved, so that breeding speed is fast, and industrialized seedling production and mass production of the anoectochilus formosanus can be realized.

The invention discloses a composition for promoting wound healing, and a preparation method thereof, and concretely belongs-the field of traditional Chinese medicinal compositions. The composition comprises, by weight, 20-30 parts of Hemsley Rockvine Root, 10-20 parts of Climbing Groundsel Herb, 5-10 parts of Isatis Root, 20-30 parts of Anoectochilus roxburghii, 10-15 parts of Euphorbia fischeriana Steud, 5-10 parts of chitosan, 3-7 parts of Weeping Forsythia, 10-18 parts of honeysuckle flowers, 5-10 parts of Setaria viridis, 5-10 parts of Herba Plantaginis, 2-8 parts of mint, 3-7 of tea leaves, 5-10 parts of Semen Momordicae, 8-16 of rose flowers, 3-7 parts of lemons and 8-15 parts of honey. The composition has the advantage of promotion of wound healing and prevention of wounds from being infected by pathogens.

The invention belongs to the field of molecular biology and particularly relates to an anoectochilus roxburghii DNA extracting method suitable for RAPD analysis. By the method, high-purity anoectochilus roxburghii DNA can be obtained at high yield according to the plant features of anoectochilus roxburghii. The method has the advantages that in traditional CTAB extract, PVP use amount is increased to 4%, and salinity is increased to 2.0mol/L; chloroform and isoamyl alcohol with the volume ratio of 24:1 are used; after isopropanol precipitates DNA, 500 microliter of high salt TE (100mmol/L Tris-HC1, pH8.0; 20mmol/L EDTA, pH8.0; 1.4mol/L NaCl) is added for reprecipitation; RNaseA is added finally. In subsequent RAPD experiments, the anoectochilus roxburghii DNA extracted by the method is clear in band, bright, stable and high in repeatability.

The invention discloses a method for cultivating wild anoectochilus formosanus, which comprises site selection, base construction, seedling cultivation, seedling treatment, field management and other steps. A standard planting mode is formed for the wild anoectochilus formosanus by automatic greenhouse management, breeding tissue culture seedling for sound seedling, seedling coating and diseases and insects prevention before transplantation, and rational and timely field management in the later period. The wild anoectochilus formosanus cultivated in the planting mode is high in survival rate, and is not easily influenced by severe field environment and diseases and insects, so that an important basis is provided for the large-scale and industrial plantation of the wild anoectochilus formosanus.

The invention relates to the technical field of plant cultivation, in particular to a cultivation and propagation method of Anoectochilus roxburghii, comprising the steps of S1, selecting an environment, to be specific, selecting a hilly land with an elevation of 300-1200 m and with gullies having forest and water at a suitable temperature of 20-28 DEG C, thereby ensuring shadiness, enough water for irrigation, wind avoiding and warmth retention in winter, less heat dissipation, constant humidity, and good traffic convenience; S2, setting up a greenhouse having height of 1.2-1.5 m, width of 6m, waist height of 1.5-1.8 m and length of 20-30 m, setting up bamboo or steel-framed seedbeds with width of about 1.2-1.5 m and height of about 80 cm within the greenhouse, providing aisles about 1 min width between the seedbeds, covering the top of the greenhouse with a layer of plastic film, and covering the plastic film with two layers of sun-shading nets and a layer of insect-proof net; S3,performing acclimation cultivation before field planting of tissue culture seedlings; S4, cultivating and propagating Anoectochilus roxburghii, to be specific, carrying out cultivation and propagation, controlling cultivation and propagation density, and determining cultivation and cultivation depth. The greenhouse is designed strictly according to the growth characteristics of Anoectochilus roxburghii; excellent growth conditions are provided to ensure the growth speed, survival rate and yield of Anoectochilus roxburghii.

The invention discloses penicillium oxalicum HY181-2, a preparation method and application thereof. The penicillium oxalicum HY181-2 is preserved in Guangdong Microbial Culture Collection Center, andthe preservation number is GDMCC No: 61142. The penicillium oxalicum HY181-2 has the effect of preventing and treating stem rot and/or anthracnose of anoectochilus formosanus.

The invention discloses evodia fruit compound essential oil with effects of alleviating pain and resisting tumors and a preparation method thereof. The essential oil is prepared from the following rawmaterials in parts by weight: 45-55 parts of evodia fruits, 5-10 parts of Chinese fan palm seeds, 3-8 parts of poria cocos, 30-40 parts of rhizoma arisaematis, 20-30 parts of anoectochilus roxburghii, 10-15 parts of turmeric root-tuber, 10-15 parts of ligusticum wallichii, 10-15 parts of turmeric and 5-10 parts of hemsley rockvine roots. The evodia fruit compound essential oil has high cell permeability, drug resistance prevention and good pain-alleviating and tumor-resisting effects and can be used for enhancing the immune modulating function due to the good synergistic effect among the ingredients.

The invention provides an organic cultivation method for anoectochilus formosanus in a sealed environment, and aims at solving the problems that in the prior art, the yield of anoectochilus formosanus is low, and management is troublesome. The invention discloses a method for organic cultivation of anoectochilus formosanus in a sealed environment. The method comprises the following steps: preparation of a cultivation medium: uniformly mixing and stirring peat, vermiculite and perlite to obtain a mixed material A; sterilizing the mixed material A; adding a probiotic liquid into sterile water, uniformly stirring to obtain a mixed diluent, spraying the mixed diluent on the mixed material A, and performing anaerobic fermentation to obtain a culture medium after the fermentation is finished; the culture medium is laid at the bottom of the transparent plastic sealing bag, the anoectochilus formosanus is planted in the transparent plastic sealing bag, and an opening in the upper end of the transparent plastic sealing bag is sealed. The transparent plastic sealing bag, the culture medium and the probiotics are matched for use, a synergistic effect is achieved, and the single-plant size and mass of anoectochilus formosanus are obviously higher than those of anoectochilus formosanus cultivated in an existing cultivation mode.

The invention discloses a solid beverage capable of alleviating hangover and protecting liver and stomach and a preparation method thereof. The solid beverage is prepared from the raw materials in parts by weight of 3-5 parts of radix puerariae, 1-2 parts of fruits, 1-2 parts of anoectochilus formosanus and 1-2 parts of chrysanthemum, and the auxiliary materials comprises 1-2 parts of rice products. The solid beverage is safe, green, healthy and free of side effects; and by integrating the effects and synergistic effects of radix puerariae, fruits, anoectochilus formosanus, chrysanthemum, rice products and the like, the solid beverage has the effects of protecting liver and stomach, nourishing qi and blood, clearing away lung-heat and relieving sore throat, moistening intestines and relaxing bowel and the like; on one hand, the solid beverage can regulate qi and blood, nourish life and prolong life; and on the other hand, blood circulation can be promoted, nutrient metabolism is improved, toxins are rapidly discharged out of a body, and body immunity is improved.

The invention belongs to the field of tumor treatment, and discloses a traditional Chinese medicine formula for treating lung cancer, which comprises the following traditional Chinese medicine components in parts by weight: 30g of anoectochilus formosanus, 20g of taxus chinensis, 30g of wheat bran, 20g of solanum lyratum, 30g of lucid ganoderma, 20g of sculellaria barbata, 20g of dindygulen peperomia herb, 20g of polygala fallax hemsl, 15g of lily, 20g of red kiwi root, 20g of cephalotaxus fortunei, 30g of bunge corydalis herb, 20g of nightshade, 10g of paris polyphylla, 20g of folium cycas, 6g of tetrastigma hemsleyanum, 30g of oldenlandia diffusa, 10g of russule, 15g of selaginella doederleinii hieron and 20g of serissa serissoides. According to the scheme, the medicines such as the anoectochilus formosanus, the taxus chinensis, the solanum lyratum, the lucid ganoderma, the sculellaria barbata, the dindygulen peperomia herb and the lily are effectively combined and support one another, so that the traditional Chinese medicine formula has the effects of strengthening the body resistance, eliminating evil, promoting blood circulation to remove blood stasis, detoxifying and removing stasis, is effectively used for treating lung cancer, and has the advantages of remarkable curative effect, small side effect, simplicity in preparation and low cost.

In order to solve the problems of poor plant growth vigour and a low light energy use ratio in a current anoectochilus roxburghii (Wall.) Lindl. culture process in the prior art, the invention provides an anoectochilus roxburghii (Wall.) Lindl. culture method capable of improving a drying rate. The anoectochilus roxburghii (Wall.) Lindl. culture method comprises the following steps of: manufacturing a culture medium; carrying out inoculation; carrying out culture: carrying out culture under a red and blue mixed illumination condition, wherein the culture is divided into three month, in the first month, illumination time is 7.5-8.5 h/d, in the second month, the illumination time is 9.5-10.5 h/d, and in the third month, the illumination time is 10.5-12.5 h/d. The culture method has the beneficial effects that red and blue light is adopted to replace traditional white light, the volume of a plant can be improved, and the growth vigour is better. In addition, effective time for the plant to absorb illumination in the first month is 7.5-8.5 h/d, the effective time for the plant to absorb the illumination in the second month is 9.5-10.5 h/d, the effective time for the plant to absorb theillumination in the third month is 10.5-12.5 h/d, and therefore, the illumination time is set into corresponding duration so as to be favorable for saving electricity energy.