Penicillium oxalicum HY181-2, preparation method and application thereof

A technology of Penicillium oxalate and its application is applied in the field of microorganisms to achieve the effects of low price, wide sources, time-saving and labor-saving production and processing

Active Publication Date: 2020-12-18
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there have been no relevant reports and patents on the application of Penicillium oxalicum (Penicillium oxalicum), especially marine-derived Penicillium oxalicum (Penicillium oxalicum) to the prevention and treatment of Aureus aureus pests and diseases

Method used

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  • Penicillium oxalicum HY181-2, preparation method and application thereof
  • Penicillium oxalicum HY181-2, preparation method and application thereof
  • Penicillium oxalicum HY181-2, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Embodiment 1: Separation and purification of marine source Penicillium oxalicum

[0042] Crush the coral samples (coral samples from Fujian Dongshan Coral Nature Reserve) in a sterile ultra-clean bench, then mix the crushed coral samples and pour them into (PD) medium, rice washing water medium, and soil leaching solution respectively. Culture medium and corn culture medium are put into a shaker (160 rpm) and cultured at 28° C. for 2-3 days. Take out the shaker flask, use the plate dilution coating method on the culture solution, and put the coated plate into a 28°C incubator to incubate in the dark. Take out the plates every day for bacteria screening: separate the colonies with different lengths in different plates of the same sample, or the strains that have inhibitory effect on other surrounding strains, and take pictures to record the growth of the selected strains. From the isolated strains, a fungus was found and named HY181-2, and other strains around it were d...

Embodiment 2

[0043] Example 2: Identification of Penicillium oxalicum of marine origin

[0044] (1) Morphological characteristics of bacteria

[0045] Morphological identification of strain HY181-2: inoculate the purified strain HY181-2 into PDA medium, culture in a 28°C incubator in the dark for 4-5 days, observe and record the characteristics of each colony, and take photos and culture under a microscope Characters and related literature were identified. see results figure 1 . Among them, A is the front photo of HY181-2 colony, B is the back photo of HY181-2 colony, and C is the photomicrograph of strain HY181-2 mycelia and spores.

[0046] See the strain HY181-2 on the PDA plate figure 1 A and B. It can be seen that the colony is relatively flat as a whole, and there are obvious small rings in the middle of the colony. The colony spreads like a carpet, and there is a circle of white hyphae around the colony, which is velvety in texture. The middle of the colony is turquoise, and th...

Embodiment 3

[0059] Example 3 Antagonism between Penicillium oxalicum from marine sources and Fusarium oxysporum, the pathogenic bacterium of clematis stem rot

[0060] The comparative bacterial strain of following embodiment: clematis stem rot pathogenic bacterium Fusarium oxysporum (Fusarium oxysporum) JXL (hereinafter referred to as pathogenic bacterium JXL) and anthracnose pathogenic bacterium gliosporum anthracnose, have another name called disc long spore-shaped thorn sporum (Colletotrichum gloeosporioides) TJ (hereinafter referred to as pathogenic bacteria TJ), was isolated, purified and preserved from the diseased plants of clematis collected from the planting bases of clematis in Xiamen, Zhangzhou and Longyan in our laboratory. After shape comparison, the pathogenic bacteria JXL is the same strain as the strain preservation number CICC 41029, and the pathogenic bacteria TJ is the same bacterial strain as the strain preservation number CGMCC3.17360.

[0061] Pathogen JXL: strain de...

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Abstract

The invention discloses penicillium oxalicum HY181-2, a preparation method and application thereof. The penicillium oxalicum HY181-2 is preserved in Guangdong Microbial Culture Collection Center, andthe preservation number is GDMCC No: 61142. The penicillium oxalicum HY181-2 has the effect of preventing and treating stem rot and/or anthracnose of anoectochilus formosanus.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Penicillium oxalicum HY181-2, a preparation method and an application thereof. Background technique [0002] Clematis clematis is a perennial herb. It is a rare folk medicinal material in my country. The whole plant can be used as medicine. It is known as "King of Medicine" and "Golden Grass". It is produced in Zhejiang, Fujian, Hunan, Guangdong, Hainan and other places in my country. Because of its high medicinal value, the mining of wild clematis is serious, and the wild clematis is on the verge of extinction. Therefore, artificially cultivated clematis has become the main source of the market today. With the development of the artificial cultivation industry of clematis, the related diseases of clematis have become more and more prominent. During the artificial cultivation process in Fujian Province, it was found that the diseases of clematis mainly include anthracnose, soft ro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12N11/14A01N63/36A01P3/00C12R1/80
CPCC12N1/14C12N1/02C12N11/14A01N63/36C12R2001/80C12N1/145
Inventor 林文珍林志楷郭迟鸣郭莺刘黎卿
Owner SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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