Theanine composition with anti-oxidation and anti-wrinkle effects

Abstract

The invention relates to the technical field of cosmetics, in particular to a theanine composition with antioxidant and anti-wrinkle effects. The theanine composition provided by the invention is prepared from the following components in parts by weight: 0.5 to 2 parts of theanine, 0.5 to 3 parts of anoectochilus formosanus extract and 0.1 to 0.5 part of pyrus ussuriensis fruit powder. The theanine, the anoectochilus formosanus extract and the pyrus ussuriensis fruit powder are compounded, and the three components have a synergistic effect, so that the composition has excellent anti-oxidation and anti-wrinkle effects, and has a very good application prospect in the field of cosmetics.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a theanine composition with antioxidant and anti-wrinkle effects. Background Art

[0002] Theanine is a plant-based amino acid compound and a non-protein amino acid unique to tea. It possesses physiologically active properties such as antioxidant and immune-enhancing properties. Used in cosmetics, theanine can effectively scavenge free radicals, promote collagen production in the skin, maintain skin elasticity, inhibit inflammatory factors, and promote the healing of skin injuries. Currently, theanine is widely used in cosmetics. However, when used alone as an active ingredient in cosmetic formulations, its effects in free radical scavenging and photoaging resistance are relatively limited. Furthermore, when theanine is compounded with other components, due to the inherent instability of theanine's physical and chemical properties, it may be affected by the other components after compounding, resulting in its inability to exert its desired effects.

[0003] Therefore, there is an urgent need to provide a theanine composition with antioxidant and anti-wrinkle effects, good compatibility between the components in the formula, and the ability to fully exert the effects of each raw material and have a synergistic effect. Summary of the Invention

[0004] In a first aspect, the present invention provides a theanine composition with antioxidant and anti-wrinkle effects. The theanine composition comprises, by weight, 0.5-2 parts of theanine, 0.5-3 parts of roxburghii extract, and 0.1-0.5 parts of kiwi fruit powder.

[0005] Theanine, a plant-based amino acid compound and a non-protein amino acid unique to tea, possesses physiologically active properties, including antioxidant and immune-enhancing properties. Used in cosmetics, theanine effectively scavenges free radicals, promotes collagen production in the skin, and maintains skin elasticity. It also inhibits inflammatory factors and promotes the healing of skin injuries.

[0006] Anoectochilus roxburghii, a herbaceous plant of the genus Anoectochilus in the Orchidaceae family, is a traditional Chinese medicinal material with high medicinal value. Its functions include clearing heat and detoxifying, protecting the liver, nourishing the skin and skin, promoting diuresis and reducing swelling, and regulating blood sugar. Anoectochilus roxburghii extract is rich in various antioxidants (such as flavonoids and polyphenols), which can scavenge free radicals, reduce oxidative stress damage to the skin, and improve the appearance of aging skin. However, the current application of Anoectochilus roxburghii extract in cosmetics still suffers from weak antioxidant activity, which limits its application.

[0007] The Autumn Pear (Pyrus ussuriensis) is a perennial deciduous tree of the genus Pyrus in the Rosaceae family. Its benefits include clearing heat and resolving phlegm, stopping diarrhea, and reducing swelling. Autumn pear fruit powder is rich in vitamin C, polyphenols, and pectin. Vitamin C can inhibit tyrosinase activity and brighten skin tone; pectin, a natural polymer, forms a breathable protective film on the skin surface, enhancing long-lasting moisturizing. However, there are currently few reports on the application of Autumn Pear fruit powder in the existing art.

[0008] The inventors discovered that combining theanine, roxburghii extract, and kiwi fruit powder significantly enhances the antioxidant and anti-wrinkle efficacy of the composition. Furthermore, when the inventors replaced theanine, roxburghii extract, or kiwi fruit powder with components disclosed in the prior art (such as glycine, nasturtium extract, and apple extract), they found that the antioxidant and anti-wrinkle efficacy of the three-component combination was significantly reduced, far inferior to that of the three-component combination of the present invention.

[0009] In some preferred embodiments, the theanine composition comprises, by weight, 1.5 parts of theanine, 2.5 parts of roxburghii extract and 0.3 parts of kiwifruit fruit powder.

[0010] In a second aspect, the present invention provides use of the theanine composition in preparing cosmetics.

[0011] In some embodiments, the cosmetics include toners, essences, lotions, creams, masks, and makeup powder products.

[0012] In a third aspect, the present invention provides a toner comprising the theanine composition.

[0013] In some embodiments, the amount of theanine composition added to the toner is 1-6% by mass. The amount of theanine composition added can be, for example, 1%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, or a sub-range consisting of any two values ​​within the range. In some preferred embodiments, the amount of theanine composition added to the toner is 4.3% by mass.

[0014] In some embodiments, the toner further comprises at least one of a humectant and a chelating agent.

[0015] In some embodiments, the toner comprises the following components in percentage by weight: 1-6% of the theanine composition of the present invention, 5-20% of a moisturizer, 0.05-0.5% of a chelating agent, and the balance being deionized water.

[0016] In some embodiments, the moisturizing agent includes at least one of 1,2-pentanediol, propylene glycol, glycerin, butylene glycol, panthenol, and ethylhexylglycerin, and the chelating agent includes at least one of hydroxyethylethylenediaminetriacetic acid and disodium EDTA. In some preferred embodiments, the moisturizing agent includes at least one of glycerin, butylene glycol, panthenol, and ethylhexylglycerin, and the chelating agent includes disodium EDTA. In some preferred embodiments, the moisturizing agent is glycerin, butylene glycol, panthenol, and ethylhexylglycerin. The inventors have discovered that in a toner system, by selecting appropriate excipients, the active ingredients can synergistically exert their antioxidant and anti-wrinkle effects while improving the stability of the toner system, allowing it to be stably preserved during long-term storage and transportation.

[0017] In a fourth aspect, the present invention provides a method for preparing the above-mentioned toner, comprising the following steps:

[0018] The moisturizing agent, the chelating agent and the theanine composition are mixed, heated and stirred to dissolve, and then the pH value is adjusted. The mixture is cooled and filtered to obtain the toner.

[0019] In some preferred embodiments, the method for preparing the toner comprises the following steps:

[0020] S1. Add glycerol, butylene glycol and disodium EDTA to water, heat and stir to dissolve;

[0021] S2. Then add the autumn pear fruit powder, theanine and the roxburghii extract and stir to dissolve, then add panthenol and ethylhexylglycerin and stir evenly, then adjust the pH value, cool and filter to obtain the toner.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The invention compounds theanine, roxburghii extract and kiwi fruit powder, and the three components synergize to enhance the effect, thereby having excellent antioxidant and anti-wrinkle effects and having good application prospects in the field of cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is the product photo of Toner 1. DETAILED DESCRIPTION

[0025] The present invention is further described in detail below through specific examples. The specific examples do not limit the scope of protection of the present invention. Some non-essential modifications and adjustments made by others based on the concept of the present invention still fall within the scope of protection of the present invention. In the following examples and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified.

[0026] Theanine was purchased from Sichuan Chengzhu Biotechnology Co., Ltd. with a CAS number of 3081-61-6.

[0027] Anoectochilus roxburghii extract was purchased from Baoji Liupanyun Biotechnology Co., Ltd.

[0028] Qiuzi pear fruit powder was purchased from Shaanxi Lvlai Biotechnology Co., Ltd.

[0029] Glycine was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0030] Trollius extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd.

[0031] Apple extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0032] The components and weight parts of the compositions of the Examples and Comparative Examples are shown in Table 1.

[0033] Table 1

[0034]

[0035] Note: " / " in Table 1 indicates no addition. Glycine promotes collagen production, improves dry and rough skin, and has moisturizing and antioxidant properties. Nasturtium flower extract contains flavonoids, alkaloids, and other ingredients with antioxidant and antibacterial properties. Apple extract is a natural plant extract extracted from apple fruit. Its main components include polyphenols, vitamin C, natural fruit acids, and minerals, and have moisturizing and antioxidant properties.

[0036] Test Example 1: Photoaging Repair Efficacy Test

[0037] Ultraviolet radiation is one of the main environmental factors contributing to skin aging. Long-term exposure to UV radiation can lead to photoaging, manifested by sagging, dull skin tone, decreased elasticity, and deeper and thicker wrinkles. Oxidative stress is the initiator of photoaging, generating a large amount of reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide, singlet oxygen, and hydroxyl radicals. Reducing the accumulation of ROS and maintaining a stable antioxidant environment are crucial for protecting the skin from UV damage.

[0038] Test sample: 2% by mass Example 1-6 was prepared with the composition of Comparative Example 1-6 using DMEM medium containing 10% fetal bovine serum as a solvent.

[0039] Experimental steps: HaCaT human keratinocytes were seeded into 96-well plates (10,000 cells / well) and cultured in an incubator at 37°C for 24 hours. After the culture was completed, the original culture medium in the wells was discarded. DMEM culture medium containing 10% serum was added to the control group and the modeling group, and the test sample was added to each well of the sample group. Each group had 5 replicates and cultured for 24 hours. After the culture was completed, the supernatant was discarded and the cells were treated with 30mJ / cm 2 HaCaT human keratinocytes from the modeling and sample groups were irradiated with ultraviolet B (UVB) for 30 minutes. The control group was cultured normally without UV irradiation. After 24 hours of incubation, the culture medium was discarded and the cells were incubated in DMEM medium containing 10% CCK8 in the dark for 2 hours. The absorbance at 450 nm was measured using a microplate reader. A higher absorbance indicates higher cell proliferation activity and better antioxidant and photoprotection efficacy.

[0040] Table 2

[0041] sample Absorbance (450nm) sample Absorbance (450nm) Example 1 1.872 Comparative Example 1 1.098 Example 2 1.817 Comparative Example 2 1.112 Example 3 1.864 Comparative Example 3 1.132 Example 4 1.823 Comparative Example 4 1.332 Example 5 1.841 Comparative Example 5 1.351 Example 6 1.855 Comparative Example 6 1.457 Modeling Group 1.021 control group 2.017

[0042] The results show that the compositions of Examples 1-6 can significantly improve the proliferation activity of UVB-damaged HaCaT cells after acting on them, which means that the compositions of Examples 1-6 have excellent antioxidant and anti-photodamage effects. When the system contains only theanine, only nasturtium extract or only kiwi fruit powder, its antioxidant and anti-photodamage effects are significantly reduced (Comparative Examples 1-3). When other components with antioxidant effects (such as glycine, nasturtium extract or apple extract) are used in the system to replace theanine, nasturtium extract or kiwi fruit powder of the present invention, its antioxidant and anti-photodamage effects are not as good as the compositions of Examples 1-6 (Comparative Examples 4-6).

[0043] Application Examples

[0044] The compositions of Examples 1-6 and Comparative Examples 1-6 were prepared into toners, which were respectively referred to as Toner 1-6 and Comparative Toner 1-6. The compositions of Toner 1-6 and Comparative Toner 1-3 are shown in Table 3.

[0045] Table 3

[0046]

[0047]

[0048] The preparation method of Toner 1-6 and Comparative Toner 1-3 comprises the following steps:

[0049] S1. Heat deionized water to 70°C, add glycerol, butylene glycol, and disodium EDTA in sequence while stirring, and stir until completely dissolved;

[0050] S2. Cool to 40°C, add Qiuzi pear fruit powder, stir for 30 minutes, pass through a 100-mesh sieve to ensure uniform dispersion, then add theanine and roxburghii extract, continue stirring for 20 minutes until completely dissolved, then add panthenol and ethylhexylglycerin, stir for 15 minutes until evenly mixed, slowly adjust the pH to 5.0-6.0 with 1% sodium hydroxide solution, cool to below 30°C, stop stirring, filter the material with a 0.22 μm microporous filter membrane to remove possible impurity particles, let the filtrate stand for 24 hours, observe that there is no stratification or precipitation, and then fill it to obtain the toner.

[0051] The toner 1 prepared is as follows Figure 1 shown.

[0052] The only difference between Toner 7 and Toner 1 is that an equal amount of hydroxyethylethylenediaminetriacetic acid is used to replace disodium EDTA, that is, the weight percentage of the chelating agent in Toner 7 is: 0.1% hydroxyethylethylenediaminetriacetic acid, 0% disodium EDTA, and the remaining components, dosage and preparation method are exactly the same as Toner 1.

[0053] The only difference between Toner 8 and Toner 1 is that 1,2-pentanediol is used to replace glycerin, butylene glycol, panthenol and ethylhexylglycerin. That is, the weight percentage of moisturizers in Toner 8 is: 1,2-pentanediol 9.4%, glycerin 0%, butylene glycol 0%, panthenol 0%, ethylhexylglycerin 0%. The other components, dosage and preparation method are exactly the same as Toner 1.

[0054] The only difference between Toner 9 and Toner 1 is that propylene glycol is used to replace glycerin, butylene glycol, panthenol and ethylhexylglycerin. That is, the weight percentage of moisturizers in Toner 9 is: propylene glycol 9.4%, glycerin 0%, butylene glycol 0%, panthenol 0%, ethylhexylglycerin 0%. The remaining components, dosage and preparation method are exactly the same as Toner 1.

[0055] The only difference between Toner 4 and Toner 1 is that glycine is used to replace theanine in equal amounts, and the remaining components, dosages and preparation methods are exactly the same as Toner 1.

[0056] The only difference between Toner 5 and Toner 1 is that an equal amount of golden lotus extract is used to replace the golden thread lotus extract. The other components, dosages and preparation methods are exactly the same as those of Toner 1.

[0057] The only difference between Toner 6 and Toner 1 is that apple extract is used to replace the autumn pear fruit powder in equal amounts. The other components, dosages and preparation methods are exactly the same as Toner 1.

[0058] Test Example 2: Repair and Anti-wrinkle Efficacy Test

[0059] Repair efficacy test: TewameterR TM 300 was used to test the transepidermal water loss rate (TEWL) of volunteers' skin.

[0060] Anti-wrinkle efficacy test: Use VISIA to shoot three sides, and use IPP software to analyze the wrinkle parameters (area and area ratio) of crow's feet, fine lines under the eyes and forehead wrinkles.

[0061] Test steps: 75 volunteers aged 30-60 were selected, and 5 volunteers each used a serum. The test results were averaged. Among them, the volunteers had dry and red facial skin, and the skin barrier function of the test area was poor (TEWL ≥ 15g / m 2 / h) and facial features of crow's feet, fine lines under the eyes, nasolabial folds, and forehead wrinkles. According to the "Skin Aging Atlas (Asian)", crow's feet, fine lines under the eyes, nasolabial folds, and forehead wrinkles should be ≥ Level 2, and pores should be ≥ Level 2. Before using the product, test the transepidermal water loss rate (TEWL) and wrinkle parameters, recording the obtained parameters as R0 and W0, respectively. Apply the product once daily before bed for four consecutive weeks. In the fourth week, test the TEWL rate and wrinkle parameters, recording the obtained parameters as R4 and W4, respectively. Using the calculation formula, the product's TEWL change rate and wrinkle area change rate are calculated to demonstrate the repair and anti-wrinkle improvement effects.

[0062] The calculation formulas for the change rate of transepidermal water loss and the change rate of wrinkle area are:

[0063] Transepidermal water loss rate change (%) = (R4-R0) / R0;

[0064] Wrinkle area change rate (%) = (W4-W0) / W0;

[0065] Among them, the TEWL value of the volunteers was tested before starting to use the sample (this value is recorded as R0). The volunteers took 1-2g of the sample each time and applied it evenly on the face, gently massaged for absorption, and used it once a day. After 4 weeks of continuous use, the TEWL value was tested (this value is recorded as R4). Before testing the TEWL value, the face must be cleaned with clean water, and the average value can be tested after sitting quietly in a constant temperature and humidity environment for 30 minutes.

[0066] The test results of the human body repair and anti-wrinkle effects of Toners 1-9 and Comparative Toners 1-6 are shown in Table 4.

[0067] Table 4

[0068]

[0069]

[0070] The results showed that Toners 1-9 had significant anti-wrinkle and repairing effects. However, when the toner system contained only theanine, only roxburghii extract, or only kiwi fruit powder as the active ingredient, the anti-wrinkle and repairing effects were significantly reduced (compared to Toners 1-3). When other components with antioxidant efficacy (such as glycine, truncatum extract, or apple extract) were used as active ingredients in the toner system to replace theanine, roxburghii extract, or kiwi fruit powder of the present invention, the anti-wrinkle and repairing effects were significantly inferior to Toners 1-9 (compared to Toners 4-6).

[0071] Test Example 3: Stability Test

[0072] (1) Heat resistance test: Place the test sample at 50°C for 24 hours, and then return to room temperature to observe whether the test sample shows any delamination, precipitation, or discoloration.

[0073] (2) Cold resistance test: Place the test sample at -10℃ for 24 hours. After returning to room temperature, observe whether the test sample shows any delamination, precipitation, discoloration, etc.

[0074] (3) Evaluation index: The test sample is clear and transparent, without stratification or abnormal precipitation - Grade A; the test sample changes color but does not show stratification - Grade B; the test sample changes color and shows stratification - Grade C; the test sample shows abnormal precipitation - Grade D. The evaluation results are shown in Table 5.

[0075] Table 5

[0076]

[0077]

[0078] The results show that toners 1-9 have good stability. However, when the toner system contains only theanine, only rosa roxburghii extract or only kiwi fruit powder as an active ingredient, its stability is poor (compared to toners 1-3). When other components with antioxidant efficacy (such as glycine, nasturtium extract or apple extract) are used in the toner system as active ingredients to replace theanine, rosa roxburghii extract or kiwi fruit powder of the present invention, its stability is also poor (compared to toners 4-6). This is probably because, after theanine, rosa roxburghii extract and kiwi fruit powder are compounded, they can enhance each other's stability in the system.

[0079] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A theanine composition having antioxidant and anti-wrinkle effects, characterized in that: In parts by weight, the theanine composition comprises 0.5-2 parts of theanine, 0.5-3 parts of roxburghii extract and 0.1-0.5 parts of kiwi fruit powder.

2. The theanine composition according to claim 1, wherein In parts by weight, the theanine composition comprises 1.5 parts of theanine, 2.5 parts of roxburghii extract and 0.3 parts of kiwi fruit powder.

3. Use of the theanine composition according to any one of claims 1 to 2 in the preparation of cosmetics.

4. The use according to claim 3, characterized in that The cosmetics include toners, essences, lotions, creams, masks and makeup powder products.

5. A toner, characterized in that: The invention comprises the theanine composition according to any one of claims 1 to 2.

6. The toner according to claim 5, characterized in that Calculated by mass percentage, the amount of theanine composition added to the toner is 1-6%.

7. The toner according to claim 5, characterized in that Calculated by mass percentage, the added amount of the theanine composition in the toner is 4.3%.

8. The toner according to claim 5, characterized in that The toner also includes at least one of a moisturizer and a chelating agent.

9. The toner according to claim 8, characterized in that The moisturizing agent includes at least one of 1,2-pentanediol, propylene glycol, glycerin, butylene glycol, panthenol and ethylhexylglycerin, and the chelating agent includes at least one of hydroxyethylethylenediaminetriacetic acid and disodium EDTA.

10. A method for preparing toner according to claim 8, characterized in that: The following steps are involved: The moisturizing agent, the chelating agent and the theanine composition according to any one of claims 1 to 2 are mixed, heated and stirred to dissolve, and then the pH value is adjusted. The mixture is cooled and filtered to obtain the toner.

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