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Technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.) Lindl in Fujian

A planting technology, the technology of clematis, which is applied in the field of plant tissue culture, can solve the problems of tiny clematis seeds, scarce clematis medicine sources, and inability to meet clinical needs, so as to speed up the recovery process, benefit forest ecological protection, The effect of improving economic efficiency

Active Publication Date: 2013-12-18
何碧珠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second is to solve the problem that the seeds of clematis are tiny, the embryo is not fully developed, and it is difficult to germinate under natural conditions; the source of clematis in the prior art is scarce, especially the strong analgesic, anti-inflammatory and anti-cancer substances contained in the plant. The ingredients cannot meet the clinical and daily needs of enhancing human immunity and other issues

Method used

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  • Technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.) Lindl in Fujian
  • Technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.) Lindl in Fujian
  • Technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.) Lindl in Fujian

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Materials: The stems of wild Anoectochilus plants are collected from the mountain stream at an altitude of 500m in Yongtai, Fujian. After picking, they are wrapped in wet cloth and brought back.

[0037] 2) Material disinfection treatment: Rinse the collected wild Anoectochilus with tap water, cut the stem at a distance of 2cm from the bud point, remove the excess leaves, rinse with saturated bleaching powder supernatant for 15 minutes and gently scrub with a soft brush After rinsing, place it in running water for 1 hour, then rinse with double distilled water for 3 times, disinfect with 75% alcohol in the ultra-clean workbench for 30 seconds, add 0.1% mercury for 8 minutes, and then use sterile water After flushing 4 times, the workbench is set for backup.

[0038] 3) Adventitious bud induction culture: place the sterilized material on a sterilized petri dish, use a scalpel to cut off the 0.2cm part of the incision that is in contact with the mercury, and then cut the ma...

Embodiment 2

[0068] 1) Material: The stems of wild Anoectochilus plants are collected from a mountain stream at an altitude of 300m in Yongtai, Fujian, and wrapped in a wet cloth and brought back after picking;

[0069] 2) Material disinfection treatment: Rinse the collected wild Anoectochilus with tap water, cut the stem section 1.5cm away from the bud point, remove the excess leaves, rinse with saturated bleaching powder supernatant for 15 minutes and gently use a soft brush After scrubbing and rinsing, place it in running water for 1 hour, then rinse twice with double distilled water, disinfect with 75% alcohol for 30 seconds in the ultra-clean workbench, add 0.1% mercury for 10 minutes, and then use aseptic After flushing with water 4 times, the workbench is set for standby;

[0070] 3) Adventitious bud induction culture: Place the sterilized material on a sterilized petri dish, cut off the 0.3cm part of the incision that contacts the mercury with a scalpel, and then cut the material into 1...

Embodiment 3

[0077] 1) Material: The stems of the wild Anoectochilus plant are collected from the mountain stream at an altitude of 600m in Yongtai, Fujian. After picking, they are wrapped in a wet cloth and brought back;

[0078] 2) Material disinfection treatment: Rinse the collected wild Anoectochilus with tap water, cut the stem section 1cm away from the bud point, remove the excess leaves, rinse with saturated bleaching powder supernatant for 15 minutes and gently scrub with a soft brush After rinsing, put it in running water for 0.5 hours, then rinse with double distilled water for 3 times, disinfect with 75% alcohol in the ultra-clean workbench for 30 seconds, add 0.1% mercury for 5 minutes, and then use sterile water Rinse 3 times and set the worktable for backup;

[0079] 3) Adventitious bud induction culture: Place the sterilized material on a sterilized petri dish, use a scalpel to cut off the 0.4cm part of the incision in contact with the mercury, and then cut the material into 1cm-...

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Abstract

The invention discloses a technology for field return-to-nature ex-situ plantation of wild anoectochilus roxburghii (Wall.)Lindl in Fujian. The technology comprises the steps of obtaining a material in the field, disinfecting the material, performing induction culture on adventitious buds, performing one-time grown-up seedling culture, transplanting bottle seedlings, performing underwood planting, and preparing a finished product. The technology solves the problems that the seeds of anoectochilus roxburghii (Wall.) Lindl are tiny, embryos are not completely developed, and the seeds are difficult to germinate under a natural condition; the method also solves the problems that in the prior art, a medicinal herb resource of the anoectochilus roxburghii (Wall.) Lindl is rare, and especially, the components for strongly easing pain, diminishing inflammation and resisting cancer contained in plants can not meet clinical requirements and daily requirements for enhancing human body immunity and the like. According to the technology, the wild anoectochilus roxburghii (Wall.) Lindl is cultured by a one-time grown-up seedling method and is subjected to field return-to-nature ex-situ plantation, so that the survival rate of the anoectochilus roxburghii (Wall.) Lindl is high, the yield is improved by 1-2 times, and the plants contain sufficient substances and are good in condition and quality.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a field natural return and ex situ planting technique of Fujian wild clematis. Background technique [0002] Clematis ( Anoectochilus roxburghii (Wall.) Lindl) is one of the precious herbs belonging to the Orchidaceae ( Orchidaceae ) genus ( Anoectochilus ) plant, also known as Jinxiancheng and bird ginseng, enjoys the reputation of "King of Medicine" and "Miraculous Medicine". Clematis is a terrestrial orchid, a perennial herb, 4-18cm high; rhizomes prostrate and elongated, light reddish brown, slightly fleshy, 4-9cm long, with obvious nodes, and adventitious roots on the nodes; leaves alternate, broadly ovate to Egg-shaped oval, 1.5-3.5cm long, 1-3cm wide, leaf veins reticulate, golden yellow, the underside of leaves is lavender-red, mainly grows in dense forests at an altitude of 300-600 meters or in moist grass beside streams or Under bamboo for...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 何官榕邹双全何碧珠
Owner 何碧珠
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